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RecombinantRabMAb

Recombinant Anti-Septin 2 antibody [EPR12122] (ab187654)

Reviews (1) Submit a question References (2)
Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody [EPR12122] (ab187654)
  • Western blot - Anti-Septin 2 antibody [EPR12122] (ab187654)
  • Western blot - Anti-Septin 2 antibody [EPR12122] (ab187654)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 2 antibody [EPR12122] (ab187654)
  • Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody [EPR12122] (ab187654)
  • Flow Cytometry (Intracellular) - Anti-Septin 2 antibody [EPR12122] (ab187654)
  • Anti-Septin 2 antibody [EPR12122] (ab187654)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12122] to Septin 2
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Septin 2 antibody [EPR12122]
    See all Septin 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12122] to Septin 2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, MCF7, U-87 MG, mouse NIH 3T3, mouse RAW 264.7, rat PC12 cell lysates; Human fetal brain lysate; Human breast carcinoma tissue; MCF7, Jurkat and mouse U-87 MG cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12122

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab187654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/120.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF (1)
1/100.
WB
1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/120.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/100.
WB
1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

  • Tissue specificity

    Widely expressed. Up-regulated in liver cancer.

  • Sequence similarities

    Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.
    Contains 1 septin-type G (guanine nucleotide-binding) domain.

  • Cellular localization

    Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Cleavage furrow. Midbody. Cytoplasm, cell cortex. Cell projection, cilium membrane. In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria. In epithelial cells, colocalizes with polyglutamylated tubulin around the trans-Golgi network, as well as juxatnuclear and proximal Golgi apparatus. Localizes at the base of the cilia near the morphological distinction between the cilia and plasma membranes.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DIFF6 antibody
    • hNEDD5 antibody
    • KIAA0158 antibody
    • NEDD 5 antibody
    • NEDD-5 antibody
    • NEDD5 antibody
    • Neural precursor cell expressed developmentally down regulated 5 antibody
    • Neural precursor cell expressed developmentally down regulated protein 5 antibody
    • Neural precursor cell expressed developmentally down-regulated protein 5 antibody
    • Pnutl3 antibody
    • Protein NEDD5 antibody
    • SEPT2 antibody
    • SEPT2_HUMAN antibody
    • septin 2 antibody
    • Septin-2 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody [EPR12122] (ab187654)
    Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody [EPR12122] (ab187654)

    Immunofluorescence analysis of MCF7 cells labeling Septin 2 using ab187654 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.

  • Western blot - Anti-Septin 2 antibody [EPR12122] (ab187654)
    Western blot - Anti-Septin 2 antibody [EPR12122] (ab187654)
    All lanes : Anti-Septin 2 antibody [EPR12122] (ab187654) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : U-87 MG cell lysate
    Lane 4 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

  • Western blot - Anti-Septin 2 antibody [EPR12122] (ab187654)
    Western blot - Anti-Septin 2 antibody [EPR12122] (ab187654)
    All lanes : Anti-Septin 2 antibody [EPR12122] (ab187654) at 1/1000 dilution

    Lane 1 : Raw 264.7 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa
    Observed band size: 41 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 2 antibody [EPR12122] (ab187654)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 2 antibody [EPR12122] (ab187654)

    Immunohistochemical analysis of paraffin-embedded, Human breast carcinoma tissue labeling Septin 2 with ab187654 at a 1/1000 dilution. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody [EPR12122] (ab187654)
    Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody [EPR12122] (ab187654)

    Immunofluorescence analysis of, paraformaldehyde-fixed, MCF-7 cell line labeling Septin 2 with ab187654 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200 (left panel). In blue DAPI staining (right panel).

  • Flow Cytometry (Intracellular) - Anti-Septin 2 antibody [EPR12122] (ab187654)
    Flow Cytometry (Intracellular) - Anti-Septin 2 antibody [EPR12122] (ab187654)

    Intracellular Flow Cytometry analysis of paraformaldehyde-fixed Jurkat cells labeling Septin 2 with ab187654 at a 1/120 dilution and secondary antibody FITC goat anti-rabbit IgG (red) at a 1/150 dilution, or negative control rabbit IgG (green). 

     

     

  • Anti-Septin 2 antibody [EPR12122] (ab187654)
    Anti-Septin 2 antibody [EPR12122] (ab187654)

References (2)

Publishing research using ab187654? Please let us know so that we can cite the reference in this datasheet.

ab187654 has been referenced in 2 publications.

  • Chen F  et al. FIT2 organizes lipid droplet biogenesis with ER tubule-forming proteins and septins. J Cell Biol 220:N/A (2021). PubMed: 33861319
  • Henson JH  et al. The ultrastructural organization of actin and myosin II filaments in the contractile ring: new support for an old model of cytokinesis. Mol Biol Cell 28:613-623 (2017). ICC/IF ; Sea urchin . PubMed: 28057763

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Septin 2 antibody [EPR12122]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (MDCK)
Permeabilization
Yes - 0.5% Triton X-100
Specification
MDCK
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Feb 16 2016

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