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RecombinantRabMAb

Recombinant Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)

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Western blot - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
  • Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
  • Flow Cytometry (Intracellular) - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
  • Immunoprecipitation - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
  • Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12123] to Septin 2 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, Flow Cyt (Intra), ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-Septin 2 antibody [EPR12123] - BSA and Azide free
    See all Septin 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12123] to Septin 2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cyt (Intra), ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Xenopus laevis

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250084 is the carrier-free version of ab179436.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12123

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

  • Tissue specificity

    Widely expressed. Up-regulated in liver cancer.

  • Sequence similarities

    Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.
    Contains 1 septin-type G (guanine nucleotide-binding) domain.

  • Cellular localization

    Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Cleavage furrow. Midbody. Cytoplasm, cell cortex. Cell projection, cilium membrane. In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria. In epithelial cells, colocalizes with polyglutamylated tubulin around the trans-Golgi network, as well as juxatnuclear and proximal Golgi apparatus. Localizes at the base of the cilia near the morphological distinction between the cilia and plasma membranes.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DIFF6 antibody
    • hNEDD5 antibody
    • KIAA0158 antibody
    • NEDD 5 antibody
    • NEDD-5 antibody
    • NEDD5 antibody
    • Neural precursor cell expressed developmentally down regulated 5 antibody
    • Neural precursor cell expressed developmentally down regulated protein 5 antibody
    • Neural precursor cell expressed developmentally down-regulated protein 5 antibody
    • Pnutl3 antibody
    • Protein NEDD5 antibody
    • SEPT2 antibody
    • SEPT2_HUMAN antibody
    • septin 2 antibody
    • Septin-2 antibody
    see all

Images

  • Western blot - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
    Western blot - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
    All lanes : Anti-Septin 2 antibody [EPR12123] (ab179436) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : U87-MG lysate
    Lane 3 : Jurkat lysate
    Lane 4 : MCF-7 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 41 kDa



    This data was developed using ab179436, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
    This data was developed using ab179436, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Septin 2 with ab179436 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
    This data was developed using ab179436, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Septin 2 with ab179436 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
    Immunocytochemistry/ Immunofluorescence - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
    This data was developed using ab179436, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of MCF-7 cells labeling Septin 2 with ab179436 at 1/50 dilution (red). DAPI nuclear staining (blue).
  • Flow Cytometry (Intracellular) - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
    Flow Cytometry (Intracellular) - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)

    This data was developed using ab179436, the same antibody clone in a different buffer formulation. 

    Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling Septin 2 with ab179436 at 1/10 dilution (red) compared to a rabbit IgG negative control (green). 

     

     

  • Immunoprecipitation - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
    Immunoprecipitation - Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
    This data was developed using ab179436, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (1) MCF-7 cell lysate or (2) 1XPBS (negative control) using ab179436 at a 1/10 dilution for IP, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
  • Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)
    Anti-Septin 2 antibody [EPR12123] - BSA and Azide free (ab250084)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab250084? Please let us know so that we can cite the reference in this datasheet.

ab250084 has not yet been referenced specifically in any publications.

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