Anti-Serum Amyloid A antibody [115] (ab687)

Thank you for contacting us.

I have now received further information from developer lab regarding the products ab687 and ab18711. Regrettably we will not be able to recommend these antibodies, to be used with horse samples. I apologies for any inconvenience.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for your email.

Finally, I have checked the sequence homology between human and horse SAA proteins, the homology is quite low with matching stretches.

http://www.uniprot.org/blast/uniprot/2012101621EV411P6G?alignment=27


P0DJI9 is for human SSA protein and F6PJF6 is the ID for horse protein.

In order to further check whether the antibodies ab687 and ab18711 would be suitable, I have contacted the developer laboratory of these antibodies and asked if they can match the epitope of these antibodies with horse protein sequence. I will send you the details soon.

The other antibodies we have in catalogue are mostly against the full length human proteins so I am sorry we may not be able to support the horse species cross reactivity. We however have rabbit monoclonal that have >95% immunogen sequence homology with horse so this antibody should cross react with horse.

https://www.abcam.com/Serum-Amyloid-A-antibody-EPR4134-ab108992.html


Along with ab108992 you can search an anti SAA antibody which detects N-terminal end of protein, to use these antibodies in sELISA. I am sorry we do not have N-terminal specific products.


I hope this information will nevertheless helpful. Please do not hesitate to contact me with further inquiries.

Thank you for contacting us.

We can recommend paring between ab687 and ab18711 instead of ab18713

https://www.abcam.com/serum-Amyloid-A-antibody-607-ab18711.html

https://www.abcam.com/Serum-Amyloid-A-antibody-115-ab687.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us. The antibody ab687 is reactive towards the Serum Amyloid A protein and not specific variants.. We do not have any antibodies specific for SAA1,SAA2 or SAA4.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us.

I have looked into what applications anti-Serum Amyloid A antibodies ab687 and ab81483 have been used for in order to ascertain if they are likely to detect the native epitope of Serum Amyloid A in order to perform native western blotting.

Both ab687 and ab81483 have been used in ELISA where the native epitope is presented. The ELISA carried out with ab687 was performed with the endogenously expressed protein, whilst the ELISA performed with ab81483 used recombinantly expressed protein. Both of these proteins are therefore likely to also be able to detect the protein when performing a native western blot. However, as we have not carried out this experiment I cannot guarantee that it will.

I also looked into what protocol had been used in performing immunoprecipitation with ab81483, however, the lab were unfortunately able to confirm that they had not tested the antibody in this application and that the informationhad been added to the datasheet in error. The datasheet has now been updated to reflect this.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Thank you for your phone call. 1) Regarding the image for ab1793, this was submitted by a customer as Abreview. For the details of the Abreview please follow this link: https://www.abcam.com/TNF-alpha-antibody-52B83-ab1793.html&tab=abreviews&intabreviewid=17818 It might well be that the monoclonal rabbit IgG secondary antibody is part of the DAKO EnVision+ System, Peroxidase/DAB kit mentioned in the Abreview. But unfortunately, I do not have more information in this regard. ------------------------------------------------------------------------- 2) As for the testing discount for ab687, here is the necessary information: DISCOUNT CODE: xxx Expiration date: xxx I am very pleased to hear you would like to accept our offer and test ab687 in mouse. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. I hope this information is of help to you. Please let me know if I can assist you further with our products.