Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
Recombinant

Recombinant Anti-Serum Response Factor SRF antibody [2C5] (ab252868)

Submit a review Submit a question References (2)
Western blot - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
  • Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
  • Western blot - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
  • Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
  • Anti-Serum Response Factor SRF antibody [2C5] (ab252868)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [2C5] to Serum Response Factor SRF
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Serum Response Factor SRF antibody [2C5]
    See all Serum Response Factor SRF primary antibodies

  • Description

    Rat monoclonal [2C5] to Serum Response Factor SRF

  • Host species

    Rat

  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, NIH/3T3, PC-12, HEK-293, K562 and C2C12 whole cell lysates. IP: HeLa and C2C12 whole cell lysates. IHC-P: Mouse cerebrum tissue. Mouse and rat skeletal muscle tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab252868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use a concentration of 36.067 µg/ml.
WB
Use a concentration of 1.082 µg/ml. Detects a band of approximately 67, 57, 52 kDa (predicted molecular weight: 52 kDa).
IHC-P
Use a concentration of 1.082 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IP
Use a concentration of 36.067 µg/ml.
WB
Use a concentration of 1.082 µg/ml. Detects a band of approximately 67, 57, 52 kDa (predicted molecular weight: 52 kDa).
IHC-P
Use a concentration of 1.082 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.

  • Sequence similarities

    Contains 1 MADS-box domain.

  • Post-translational
    modifications

    Phosphorylated by PRKDC.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • c fos serum response element binding factor antibody
      • c fos serum response element binding transcription factor antibody
      • ELK3 antibody
      • ERP antibody
      • MCM 1 antibody
      • MCM1 antibody
      • OTTHUMP00000039820 antibody
      • SAP2 antibody
      • Serum response factor antibody
      • SRF antibody
      • SRF serum response factor c fos serum response element binding transcription factor antibody
      • SRF_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
      Western blot - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
      All lanes : Anti-Serum Response Factor SRF antibody [2C5] (ab252868) at 0.082 µg/ml

      Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
      Lane 2 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

      Predicted band size: 52 kDa
      Observed band size: 57,67 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure times: Lane 1: 26 seconds; Lane 2: 3 minutes.

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11893590 and PMID: 10642500).

    • Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
      Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)

      Serum Response Factor SRF was immunoprecipitated from C2C12 (mouse myoblasts myoblast), whole cell lysate with ab252868 at 1/30 dilution (2µg in 0.35mg lysates) (36.067µg/ml). Western blot was performed on the immunoprecipitate using ab252868 at 1/1000 dilution. Goat Anti-rat IgG (H+L), HRP) (ab205720) was used at 1/10000 dilution.

      Lane 1: C2C12 whole cell lysate 10µg.

      Lane 2: ab252868 IP in C2C12 whole cell lysate.

      Lane 3: Rat monoclonal IgG2a (ab18450) instead of ab252868 in C2C12 whole cell lysate.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 6 seconds.

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11893590 and PMID: 10642500).

    • Western blot - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
      Western blot - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
      All lanes : Anti-Serum Response Factor SRF antibody [2C5] (ab252868) at 1.082 µg/ml

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
      Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
      Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
      Lane 4 : C2C12 (mouse myoblasts myoblast), whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

      Predicted band size: 52 kDa
      Observed band size: 52,57,67 kDa why is the actual band size different from the predicted?


      Exposure time: 20 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11893590 and PMID: 10642500). 

    • Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
      Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)

      Serum Response Factor SRF was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate with ab252868 at 1/30 dilution (2µg in 0.35mg lysates) (36.067µg/ml). Western blot was performed on the immunoprecipitate using ab252868 at 1/1000 dilution. Goat Anti-rat IgG (H+L), HRP) (ab205720) was used at 1/10000 dilution.

      Lane 1: HeLa whole cell lysate 10µg.

      Lane 2: ab252868 IP in HeLa whole cell lysate.

      Lane 3: Rat monoclonal IgG2a (ab18450) instead of ab252868 in HeLa whole cell lysate.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 10 seconds.

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11893590and PMID: 10642500).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)

      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Serum Response Factor SRF with ab252868 at 1/1000 dilution (1.082µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse cerebrum. The section was incubated with ab252868 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)

      Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Serum Response Factor SRF with ab252868 at 1/1000 dilution (1.082µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse skeletal muscle tissue. The section was incubated with ab252868 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)

      Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Serum Response Factor SRF with ab252868 at 1/1000 dilution (1.082µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on rat skeletal muscle tissue. The section was incubated with ab252868 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
      Anti-Serum Response Factor SRF antibody [2C5] (ab252868)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (2)

    Publishing research using ab252868? Please let us know so that we can cite the reference in this datasheet.

    ab252868 has been referenced in 2 publications.

    • Xu H  et al. YTHDF2 alleviates cardiac hypertrophy via regulating Myh7 mRNA decoy. Cell Biosci 11:132 (2021). PubMed: 34266473
    • Koegel H  et al. Loss of serum response factor in keratinocytes results in hyperproliferative skin disease in mice. J Clin Invest 119:899-910 (2009). PubMed: 19307725

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab252868.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com