Recombinant Anti-SET5 antibody [EPR20102] (ab202437)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20102] to SET5
- Suitable for: WB
- Knockout validated
- Reacts with: Saccharomyces cerevisiae
Related conjugates and formulations
Overview
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Product name
Anti-SET5 antibody [EPR20102]
See all SET5 primary antibodies -
Description
Rabbit monoclonal [EPR20102] to SET5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Saccharomyces cerevisiae whole cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20102 -
Isotype
IgG
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab202437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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Notes |
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WB
1/5000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
Images
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Anti-SET5 antibody [EPR20102] (ab202437) at 1/5000 dilution + Saccharomyces cerevisiae whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-SET5 antibody [EPR20102] (ab202437) at 1/5000 dilution
Lane 1 : Saccharomyces cerevisiae (WT) whole cell lysate
Lane 2 : Saccharomyces cerevisiae (SET5 KO) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Set5 KO strain (Y02901) was purchased from Euroscarf.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab202437 has not yet been referenced specifically in any publications.