Recombinant Anti-SET5 antibody [EPR20102] (ab202437)
![Western blot - Anti-SET5 antibody [EPR20102] (ab202437)](https://www.abcam.com/ps/products/202/ab202437/Images/ab202437-293014-202437wb1.jpg "Western blot - Anti-SET5 antibody [EPR20102] (ab202437)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20102] to SET5
- Suitable for: WB
- Knockout validated
- Reacts with: Saccharomyces cerevisiae
Related conjugates and formulations
Overview
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Product name
Anti-SET5 antibody [EPR20102]
See all SET5 primary antibodies -
Description
Rabbit monoclonal [EPR20102] to SET5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Saccharomyces cerevisiae whole cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20102 -
Isotype
IgG
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab202437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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| Notes |
|---|
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WB
1/5000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
Images
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Western blot - Anti-SET5 antibody [EPR20102] (ab202437)Anti-SET5 antibody [EPR20102] (ab202437) at 1/5000 dilution + Saccharomyces cerevisiae whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-SET5 antibody [EPR20102] (ab202437)](https://www.abcam.com/ps/products/202/ab202437/Images/ab202437-293013-202437wb2.jpg)
Western blot - Anti-SET5 antibody [EPR20102] (ab202437)All lanes : Anti-SET5 antibody [EPR20102] (ab202437) at 1/5000 dilution
Lane 1 : Saccharomyces cerevisiae (WT) whole cell lysate
Lane 2 : Saccharomyces cerevisiae (SET5 KO) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Set5 KO strain (Y02901) was purchased from Euroscarf.
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![Anti-SET5 antibody [EPR20102] (ab202437)](https://www.abcam.com/ps/products/202/ab202437/Images/ab202437-4-benefits-of-recombinant-antibodies.png)
Anti-SET5 antibody [EPR20102] (ab202437)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab202437 has not yet been referenced specifically in any publications.