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RecombinantRabMAb

Recombinant Anti-SET/TAF-I antibody [EPR12973] (ab181990)

Reviews (1) Submit a question References (7)
Western blot - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
  • Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
  • Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
  • Flow Cytometry (Intracellular) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
  • Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
  • Anti-SET/TAF-I antibody [EPR12973] (ab181990)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12973] to SET/TAF-I
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE

Overview

  • Product name

    Anti-SET/TAF-I antibody [EPR12973]
    See all SET/TAF-I primary antibodies

  • Description

    Rabbit monoclonal [EPR12973] to SET/TAF-I

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat , 293T, HeLa, HepG2 whole cell lysate (ab7900); Human nephroblastoma and rat spleen tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/10000 - 1/100000. Detects a band of approximately 37, 39 kDa (predicted molecular weight: 33 kDa).
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/1000.
IP
1/30.
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Notes
WB
1/10000 - 1/100000. Detects a band of approximately 37, 39 kDa (predicted molecular weight: 33 kDa).
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/1000.
IP
1/30.
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.

  • Tissue specificity

    Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.

  • Involvement in disease

    Note=A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with NUP214/CAN.

  • Sequence similarities

    Belongs to the nucleosome assembly protein (NAP) family.

  • Domain

    The C-terminal acidic domain mediates the inhibition of histone acetyltransferases and is required for the DNA replication stimulatory activity.

  • Post-translational
    modifications

    Isoform 2 is phosphorylated on Ser-15 and Thr-23.
    Isoform 2 is acetylated on Lys-11.
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
    N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated.

  • Cellular localization

    Cytoplasm > cytosol. Endoplasmic reticulum. Nucleus > nucleoplasm. In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. Following CTL attack, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium. The SET complex is associated with the endoplasmic reticulum.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 2PP2A antibody
    • HLA DR associated protein II antibody
    • HLA-DR-associated protein II antibody
    • I 2PP2A antibody
    • I-2PP2A antibody
    • I2PP2A antibody
    • IGAAD antibody
    • Inhibitor of granzyme A activated DNase antibody
    • Inhibitor of granzyme A-activated DNase antibody
    • Inhibitor of GzmA-activated DNase antibody
    • inhibitor-2 of protein phosphatase-2A antibody
    • IPP2A2 antibody
    • PHAPII antibody
    • Phosphatase 2A inhibitor I2PP2A antibody
    • protein phosphatase type 2A inhibitor antibody
    • Protein SET antibody
    • Set antibody
    • SET nuclear oncogene antibody
    • SET translocation antibody
    • SET translocation (myeloid leukemia-associated) antibody
    • SET_HUMAN antibody
    • TAF I antibody
    • TAF IBETA antibody
    • TAF-I antibody
    • TAFI antibody
    • Template activating factor I antibody
    • Template-activating factor I antibody
    • Template-Activating Factor-I, chromatin remodelling factor antibody
    see all

Images

  • Western blot - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    Western blot - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    All lanes : Anti-SET/TAF-I antibody [EPR12973] (ab181990) at 1/100000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Hela cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa



    Blocking/dilution buffer and concentration: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling SET/TAF-I with Purified ab181990 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)

    Immunohistochemical analysis of paraffin-embedded Human nephroblastoma tissue labeling SET/TAF-I with ab181990 at a 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling SET/TAF-I with ab181990 at a 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    Immunocytochemistry/ Immunofluorescence - Anti-SET/TAF-I antibody [EPR12973] (ab181990)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cell line staining SET/TAF-I using ab181990 at 1/1000 dilution. A Dylight 555 conjugated Goat anti rabbit IgG at 1/200 dilution was used for secondary antibody detection. Counter stain: Dapi.

  • Flow Cytometry (Intracellular) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    Flow Cytometry (Intracellular) - Anti-SET/TAF-I antibody [EPR12973] (ab181990)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Hela cells labeling SET/TAF-I using ab181990at a 1/10 dilution, or a rabbit monoclonal IgG isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution. 

     

     

  • Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    Immunoprecipitation - Anti-SET/TAF-I antibody [EPR12973] (ab181990)

    Immunoprecipitation of Jurkat cell lysate labeling SET/TAF-I using ab181990 at a 1/30 dilution (lane 1). A goat anti-rabbit IgG, (H+L), peroxidase conjugated secondary antibody was used at a dilution of 1/1000. Blocking/dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-SET/TAF-I antibody [EPR12973] (ab181990)
    Anti-SET/TAF-I antibody [EPR12973] (ab181990)

References (7)

Publishing research using ab181990? Please let us know so that we can cite the reference in this datasheet.

ab181990 has been referenced in 7 publications.

  • Höllmüller E  et al. Site-specific ubiquitylation acts as a regulator of linker histone H1. Nat Commun 12:3497 (2021). PubMed: 34108453
  • Mandemaker IK  et al. Histone H1 eviction by the histone chaperone SET reduces cell survival following DNA damage. J Cell Sci 133:N/A (2020). PubMed: 32184266
  • Liu Y  et al. TAF-Iß deficiency inhibits proliferation and promotes apoptosis by rescuing PP2A and inhibiting the AKT/GSK-3ß pathway in leukemic cells. Exp Ther Med 18:3801-3808 (2019). PubMed: 31611934
  • Yang Z  et al. A pilot study on polycystic ovarian syndrome caused by neonatal exposure to tributyltin and bisphenol A in rats. Chemosphere 231:151-160 (2019). PubMed: 31129395
  • Ma W & Mayr C A Membraneless Organelle Associated with the Endoplasmic Reticulum Enables 3'UTR-Mediated Protein-Protein Interactions. Cell 175:1492-1506.e19 (2018). PubMed: 30449617
  • Ma J  et al. Rivaroxaban attenuates thrombosis by targeting the NF-?B signaling pathway in a rat model of deep venous thrombus. Int J Mol Med 40:1869-1880 (2017). PubMed: 29039441
  • Berkovits BD & Mayr C Alternative 3' UTRs act as scaffolds to regulate membrane protein localization. Nature 522:363-7 (2015). WB, Flow Cyt . PubMed: 25896326

Customer reviews and Q&As

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Western blot abreview for Anti-SET/TAF-I antibody [EPR12973]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Leukemic Cancer Cell lines)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Leukemic Cancer Cell lines
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Dec 06 2017

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