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RecombinantRabMAb

Recombinant Anti-SFPQ antibody [EPR11847] (ab177149)

Reviews (2) Submit a question References (10)
Western blot - Anti-SFPQ antibody [EPR11847] (ab177149)
  • Immunocytochemistry/ Immunofluorescence - Anti-SFPQ antibody [EPR11847] (ab177149)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] (ab177149)
  • Flow Cytometry - Anti-SFPQ antibody [EPR11847] (ab177149)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] (ab177149)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] (ab177149)
  • Anti-SFPQ antibody [EPR11847] (ab177149)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11847] to SFPQ
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 APC Carrier Free HRP PE

Overview

  • Product name

    Anti-SFPQ antibody [EPR11847]
    See all SFPQ primary antibodies

  • Description

    Rabbit monoclonal [EPR11847] to SFPQ

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human and Rat colon and Mouse testis tissue; ICC/IF: NIH/3T3 cells; Flow Cyt: HeLa cells. WB: HeLa, NIH/3T3 and C2C12 whole cell lysates. Rat brain lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11847

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/50.
WB (1)
1/10000 - 1/50000. Detects a band of approximately 100 kDa (predicted molecular weight: 76 kDa).
IHC-P
1/10000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.
ICC/IF (1)
1/50 - 1/100.
Notes
Flow Cyt
1/50.
WB
1/10000 - 1/50000. Detects a band of approximately 100 kDa (predicted molecular weight: 76 kDa).
IHC-P
1/10000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.
ICC/IF
1/50 - 1/100.

Target

  • Function

    DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.

  • Involvement in disease

    Note=A chromosomal aberration involving SFPQ may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;1)(p11.2;p34) with TFE3.

  • Sequence similarities

    Contains 2 RRM (RNA recognition motif) domains.

  • Post-translational
    modifications

    The N-terminus is blocked.
    Phosphorylated on multiple serine and threonine residues during apoptosis. In vitro phosphorylated by PKC. Phosphorylation stimulates binding to DNA and D-loop formation, but inhibits binding to RNA.
    Arg-7, Arg-9, Arg-19 and Arg-25 are dimethylated, probably to asymmetric dimethylarginine.

  • Cellular localization

    Nucleus matrix. Predominantly in nuclear matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 100 kDa DNA pairing protein antibody
    • 100 kDa DNA-pairing protein antibody
    • 100 kDa subunit antibody
    • DNA binding p52/p100 complex 100 kDa subunit antibody
    • DNA-binding p52/p100 complex antibody
    • hPOMp100 antibody
    • Polypyrimidine tract binding protein associated splicing factor antibody
    • Polypyrimidine tract-binding protein-associated-splicing factor antibody
    • POMP100 antibody
    • PPP1R140 antibody
    • proline- and glutamine-rich antibody
    • Protein phosphatase 1 regulatory subunit 140 antibody
    • PSF antibody
    • PTB associated splicing factor antibody
    • PTB-associated-splicing factor antibody
    • Sfpq antibody
    • SFPQ_HUMAN antibody
    • Splicing factor antibody
    • Splicing factor proline and glutamine rich antibody
    • Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated) antibody
    • Splicing factor proline/glutamine rich antibody
    see all

Images

  • Western blot - Anti-SFPQ antibody [EPR11847] (ab177149)
    Western blot - Anti-SFPQ antibody [EPR11847] (ab177149)
    All lanes : Anti-SFPQ antibody [EPR11847] (ab177149) at 1/10000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 3 : C2C12 (Mouse myoblasts myoblast) whole cell lysate
    Lane 4 : Rat brain lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 76 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SFPQ antibody [EPR11847] (ab177149)
    Immunocytochemistry/ Immunofluorescence - Anti-SFPQ antibody [EPR11847] (ab177149)

    Immunocytochemistry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling SFPQ with purified ab177149 at 1/50 dilution (9.7 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] (ab177149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] (ab177149)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling SFPQ with purified ab177149 at 1/10000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Flow Cytometry - Anti-SFPQ antibody [EPR11847] (ab177149)
    Flow Cytometry - Anti-SFPQ antibody [EPR11847] (ab177149)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling SFPQ with purified ab177149 at 1/50 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] (ab177149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] (ab177149)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling SFPQ with purified ab177149 at 1/10000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] (ab177149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] (ab177149)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue sections labeling SFPQ with purified ab177149 at 1/10000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Anti-SFPQ antibody [EPR11847] (ab177149)
    Anti-SFPQ antibody [EPR11847] (ab177149)

References (10)

Publishing research using ab177149? Please let us know so that we can cite the reference in this datasheet.

ab177149 has been referenced in 10 publications.

  • Wang S  et al. Long non-coding RNA SNHG1 suppresses cell migration and invasion and upregulates SOCS2 in human gastric carcinoma. Biochem Biophys Rep 27:101052 (2021). PubMed: 34179518
  • Song H  et al. SFPQ Is an FTO-Binding Protein that Facilitates the Demethylation Substrate Preference. Cell Chem Biol 27:283-291.e6 (2020). PubMed: 31981477
  • Mora Gallardo C  et al. Dido3-dependent SFPQ recruitment maintains efficiency in mammalian alternative splicing. Nucleic Acids Res 47:5381-5394 (2019). PubMed: 30931476
  • An H  et al. Stress granules regulate stress-induced paraspeckle assembly. J Cell Biol 218:4127-4140 (2019). PubMed: 31636118
  • An H  et al. ALS-linked FUS mutations confer loss and gain of function in the nucleus by promoting excessive formation of dysfunctional paraspeckles. Acta Neuropathol Commun 7:7 (2019). PubMed: 30642400
  • Menoni H  et al. The transcription-coupled DNA repair-initiating protein CSB promotes XRCC1 recruitment to oxidative DNA damage. Nucleic Acids Res 46:7747-7756 (2018). PubMed: 29955842
  • Ru Y  et al. NEAT1_2-SFPQ axis mediates cisplatin resistance in liver cancer cells in vitro. Onco Targets Ther 11:5695-5702 (2018). PubMed: 30254462
  • Shelkovnikova TA  et al. Protective paraspeckle hyper-assembly downstream of TDP-43 loss of function in amyotrophic lateral sclerosis. Mol Neurodegener 13:30 (2018). PubMed: 29859124
  • Tao Y  et al. MicroRNA-1296 Facilitates Proliferation, Migration And Invasion Of Colorectal Cancer Cells By Targeting SFPQ. J Cancer 9:2317-2326 (2018). PubMed: 30026827
  • Seo J  et al. Oxidative Stress Triggers Body-Wide Skipping of Multiple Exons of the Spinal Muscular Atrophy Gene. PLoS One 11:e0154390 (2016). WB . PubMed: 27111068

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-SFPQ antibody [EPR11847]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Permeabilization
Yes - 0.3% Triton-X100 in blocking buffer
Specification
HEK293
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 30 2018

Western blot abreview for Anti-SFPQ antibody [EPR11847]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12% Bis-Tris)
Loading amount
25 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 12 2018

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