Recombinant Anti-SFT antibody [EPR13000(B)] (ab176561)
![Western blot - Anti-SFT antibody [EPR13000(B)] (ab176561)](https://www.abcam.com/ps/products/176/ab176561/Images/ab176561-258311-anti-sft-antibody-epr13000b-western-blot.jpg "Western blot - Anti-SFT antibody [EPR13000(B)] (ab176561)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13000(B)] to SFT
- Suitable for: Flow Cyt (Intra), WB, IHC-P, IP
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-SFT antibody [EPR13000(B)]
See all SFT primary antibodies -
Description
Rabbit monoclonal [EPR13000(B)] to SFT -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat and Raji cell lysates, Human skeletal muscle lysate, Human thryoid gland carcinoma and prostate tissues, Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13000(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab176561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/10000 - 1/50000. Predicted molecular weight: 17 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
1/10 - 1/100.
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| Notes |
|---|
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Predicted molecular weight: 17 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
1/10 - 1/100. |
Target
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Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of CHIP, TRAF6 and TRIM63/MURF1. Ubiquitinates CHIP-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4. -
Tissue specificity
Ubiquitous. Up-regulated in livers of iron-overloaded patients with hereditary hemochromatosis. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7321 Human
- Entrez Gene: 216080 Mouse
- Entrez Gene: 361831 Rat
- Omim: 602961 Human
- SwissProt: P51668 Human
- SwissProt: P61080 Mouse
- SwissProt: D3ZDK2 Rat
- Unigene: 129683 Human
see all -
Alternative names
- E2(17)KB1 antibody
- SFT antibody
- Stimulator of Fe transport antibody
see all
Images
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Western blot - Anti-SFT antibody [EPR13000(B)] (ab176561)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SFT knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab176561 observed at 18 kDa. Red - loading control, ab181602, observed at 37 kDa. ab176561 was shown to recognize SFT when SFT knockout samples were used, along with additional cross-reactive bands. Wild-type and SFT knockout samples were subjected to SDS-PAGE. ab176561 and ab181602 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-SFT antibody [EPR13000(B)] (ab176561)](https://www.abcam.com/ps/products/176/ab176561/Images/ab176561-204224-anti-sft-antibody-epr13000b-western-blot.jpg)
Western blot - Anti-SFT antibody [EPR13000(B)] (ab176561)All lanes : Anti-SFT antibody [EPR13000(B)] (ab176561) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Raji cell lysate
Lane 3 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 17 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFT antibody [EPR13000(B)] (ab176561)](https://www.abcam.com/ps/products/176/ab176561/Images/ab176561-204223-anti-sft-antibody-epr13000b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFT antibody [EPR13000(B)] (ab176561)Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human prostate tissue labeling SFT with ab176561 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFT antibody [EPR13000(B)] (ab176561)](https://www.abcam.com/ps/products/176/ab176561/Images/ab176561-204222-anti-sft-antibody-epr13000b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFT antibody [EPR13000(B)] (ab176561)Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human thyroid gland carcinoma tissue labeling SFT with ab176561 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-SFT antibody [EPR13000(B)] (ab176561)](https://www.abcam.com/ps/products/176/ab176561/Images/ab176561-204221-anti-sft-antibody-epr13000b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SFT antibody [EPR13000(B)] (ab176561)Intracellular Flow Cytometry analysis of permeabilized Jurkat cells labeling SFT (red) with ab176561 at a 1/10 dilution, or negative control rabbit IgG (green)
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![Immunoprecipitation - Anti-SFT antibody [EPR13000(B)] (ab176561)](https://www.abcam.com/ps/products/176/ab176561/Images/ab176561-204220-anti-sft-antibody-epr13000b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SFT antibody [EPR13000(B)] (ab176561)Western blot analysis on immunoprecipitation pellet from Human skeletal muscle lysate labeling SFT with ab176561 at 1/10 dilution.
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![Anti-SFT antibody [EPR13000(B)] (ab176561)](https://www.abcam.com/ps/products/176/ab176561/Images/ab176561-7-benefits-of-recombinant-antibodies.png)
Anti-SFT antibody [EPR13000(B)] (ab176561)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab176561 has been referenced in 3 publications.
- Brühl J et al. The DNA repair protein SHPRH is a nucleosome-stimulated ATPase and a nucleosome-E3 ubiquitin ligase. Epigenetics Chromatin 12:52 (2019). PubMed: 31434570
- Shi Y et al. Ube2D3 and Ube2N are essential for RIG-I-mediated MAVS aggregation in antiviral innate immunity. Nat Commun 8:15138 (2017). WB ; Human . PubMed: 28469175
- Liu XJ et al. Human cytomegalovirus IE1 downregulates Hes1 in neural progenitor cells as a potential E3 ubiquitin ligase. PLoS Pathog 13:e1006542 (2017). PubMed: 28750047