Recombinant Anti-SHP1 antibody [EPR5519] (ab124942)
![Western blot - Anti-SHP1 antibody [EPR5519] (ab124942)](https://www.abcam.com/ps/products/124/ab124942/Images/ab124942-454786-anti-shp1-antibody-epr5519-western-blot-wildtype-thp1-ptpn6-knockout-thp1-jurkat-k562.jpg "Western blot - Anti-SHP1 antibody [EPR5519] (ab124942)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5519] to SHP1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SHP1 antibody [EPR5519]
See all SHP1 primary antibodies -
Description
Rabbit monoclonal [EPR5519] to SHP1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human SHP1 aa 1-100. The exact sequence is proprietary.
Database link: P29350 -
Positive control
- IHC-P: Human tonsil tissue. WB: THP1, Jurkat, Raji and lymph node lysates. ICC: Raji cells. Flow Cyt (intra): Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.95 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5519 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 68 kDa.
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| IHC-P |
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/250 - 1/500.
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| Notes |
|---|
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 68 kDa. |
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IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/250 - 1/500. |
Target
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Function
Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. -
Tissue specificity
Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain. -
Post-translational
modificationsPhosphorylated on serine and tyrosine residues. -
Cellular localization
Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II. - Information by UniProt
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Database links
- Entrez Gene: 5777 Human
- Omim: 176883 Human
- SwissProt: P29350 Human
- Unigene: 63489 Human
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Alternative names
- 70Z-SHP antibody
- EC 3.1.3.48 antibody
- HCP antibody
see all
Images
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Western blot - Anti-SHP1 antibody [EPR5519] (ab124942)All lanes : Anti-SHP1 antibody [EPR5519] (ab124942) at 1/1000 dilution
Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : PTPN6 knockout THP-1 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab124942 observed at 70 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab124942 was shown to react with SHP1 in wild-type THP-1 cells in Western blot with loss of signal observed in PTPN6 knockout sample. Wild-type THP-1 and PTPN6 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab124942 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [EPR5519] (ab124942)](https://www.abcam.com/ps/products/124/ab124942/Images/ab124942-138984-anti-shp1-antibody-epr5519-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [EPR5519] (ab124942)ab124942, at a 1/1000 dilution, staining SHP1 in paraffin-embedded Human tonsil tissue by immunohistochemistry
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-SHP1 antibody [EPR5519] (ab124942)](https://www.abcam.com/ps/products/124/ab124942/Images/ab124942-261740-anti-shp1-antibody-epr5519-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SHP1 antibody [EPR5519] (ab124942)Immunocytochemistry/Immunofluorescence analysis of Raji (human Burkitt's lymphoma) cells labelling SHP1 with purified ab124942 at a dilution of 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at dilution of 1/1000 was used as the secondary antibody. Nuclei counterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
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![Western blot - Anti-SHP1 antibody [EPR5519] (ab124942)](https://www.abcam.com/ps/products/124/ab124942/Images/ab124942-138985-anti-shp1-antibody-epr5519-western-blot.jpg)
Western blot - Anti-SHP1 antibody [EPR5519] (ab124942)All lanes : Anti-SHP1 antibody [EPR5519] (ab124942) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Raji cell lysate
Lane 3 : lymph node lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 68 kDa -
![Flow Cytometry (Intracellular) - Anti-SHP1 antibody [EPR5519] (ab124942)](https://www.abcam.com/ps/products/124/ab124942/Images/ab124942-6-anti-shp1-antibody-epr5519-flowcytometry-jurkat-human.jpg)
Flow Cytometry (Intracellular) - Anti-SHP1 antibody [EPR5519] (ab124942)Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling SHP1 with purified ab124942 at 1/100 dilution (10µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
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![OI-RD Scanning - Anti-SHP1 antibody [EPR5519] (ab124942)](https://www.abcam.com/ps/products/124/ab124942/Images/ab124942-138983-anti-shp1-antibody-epr5519.jpg)
OI-RD Scanning - Anti-SHP1 antibody [EPR5519] (ab124942) -
![Anti-SHP1 antibody [EPR5519] (ab124942)](https://www.abcam.com/ps/products/124/ab124942/Images/ab124942-5-benefits-of-recombinant-antibodies.png)
Anti-SHP1 antibody [EPR5519] (ab124942)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab124942 has been referenced in 9 publications.
- Bian Y et al. SMURF1-mediated ubiquitylation of SHP-1 promotes cell proliferation and invasion of endometrial stromal cells in endometriosis. Ann Transl Med 9:362 (2021). PubMed: 33842583
- Tian W et al. A Novel Prognostic Tool for Glioma Based on Enhancer RNA-Regulated Immune Genes. Front Cell Dev Biol 9:798445 (2021). PubMed: 35127714
- Chen X et al. Ropivacaine inhibits cervical cancer cell growth via suppression of the miR-96/MEG2/pSTAT3 axis. Oncol Rep 43:1659-1668 (2020). PubMed: 32323811
- Cui X et al. FCRL3 promotes IL-10 expression in B cells through the SHP-1 and p38 MAPK signaling pathways. Cell Biol Int N/A:N/A (2020). PubMed: 32374464
- Koh JS et al. Inhibition of STAT3 in gastric cancer: role of pantoprazole as SHP-1 inducer. Cell Biosci 8:50 (2018). PubMed: 30202514
- Yao D et al. CD47 is associated with the up-regulation of the PD-1 oncogenic signaling pathway. Int J Clin Exp Pathol 11:5612-5621 (2018). PubMed: 31949648
- Gladkikh AA et al. Comparison of the mRNA expression profile of B-cell receptor components in normal CD5-high B-lymphocytes and chronic lymphocytic leukemia: a key role of ZAP70. Cancer Med 6:2984-2997 (2017). PubMed: 29125235
- Raymond AA et al. Reptin regulates insulin-stimulated Akt phosphorylation in hepatocellular carcinoma via the regulation of SHP-1/PTPN6. Cell Biochem Funct 35:289-295 (2017). PubMed: 28833338
- Hagberg N et al. Systemic lupus erythematosus immune complexes increase the expression of SLAM family members CD319 (CRACC) and CD229 (LY-9) on plasmacytoid dendritic cells and CD319 on CD56(dim) NK cells. J Immunol 191:2989-98 (2013). Human . PubMed: 23956418