Name: Anti-SHP1 antibody [Y476]
Brand: RabMAb
SKU: ab32559
Rating: 5 (3 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] (ab32559)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Y476] to SHP1
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human

Alexa Fluor® 555 Carrier Free HRP PE

Product name

Anti-SHP1 antibody [Y476]
See all SHP1 primary antibodies
Description

Rabbit monoclonal [Y476] to SHP1
Host species

Rabbit
Specificity

The antibody is predicted to detect isoforms 1, 2 and 3 of human SHP1 based on sequence analysis.
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human SHP1 aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P29350
Positive control
- WB: THP-1 cell lysate, A431 cell lysate, Jurkat cell lysate, K562 cell lysate. IHC-P: Human tonsil and lymph node tissue; Rat spleen tissue; Mouse liver tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

Y476
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant human SHP1 protein (ab51289)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(2)	1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 68 kDa).
IHC-P		1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
WB 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Application notes

Is unsuitable for Flow Cyt,ICC/IF or IP.

Function

Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
Tissue specificity

Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain.
Post-translational
modifications

Phosphorylated on serine and tyrosine residues.
Cellular localization

Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
Target information above from: UniProt accession P29350 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 5777 Human
- Entrez Gene: 15170 Mouse
- Entrez Gene: 116689 Rat
- Omim: 176883 Human
- SwissProt: P29350 Human
- SwissProt: P29351 Mouse
- SwissProt: P81718 Rat
- Unigene: 63489 Human
- Unigene: 271799 Mouse
- Unigene: 18985 Rat
see all
Alternative names
- 70Z-SHP antibody
- EC 3.1.3.48 antibody
- HCP antibody
- HCPH antibody
- Hematopoietic cell phosphatase antibody
- Hematopoietic cell protein tyrosine phosphatase antibody
- Hematopoietic cell protein-tyrosine phosphatase antibody
- HPTP1C antibody
- Protein tyrosine phosphatase 1C antibody
- Protein tyrosine phosphatase non receptor type 6 antibody
- Protein tyrosine phosphatase SHP1 antibody
- Protein-tyrosine phosphatase 1C antibody
- protein-tyrosine phosphatase SHP 1 antibody
- Protein-tyrosine phosphatase SHP-1 antibody
- PTN6_HUMAN antibody
- PTP 1C antibody
- PTP-1C antibody
- PTP1C antibody
- Ptpn6 antibody
- SH PTP 1 antibody
- SH PTP1 antibody
- SH-PTP1 antibody
- SHP 1 antibody
- SHP 1L antibody
- SHP1 antibody
- SHP1L antibody
- tyrosine protein phosphatase non receptor type 6 antibody
- Tyrosine-protein phosphatase non-receptor type 6 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] (ab32559)

Immunohistochemical staining of paraffin embedded human tonsil with purified ab32559 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-SHP1 antibody [Y476] (ab32559)

All lanes : Anti-SHP1 antibody [Y476] (ab32559) at 1/1000 dilution

Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : PTPN6 knockout THP-1 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 68 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab32559 observed at 70 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab32559 was shown to react with SHP1 in wild-type THP-1 cells in Western blot with loss of signal observed in PTPN6 knockout sample. Wild-type THP-1 and PTPN6 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab32559 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] (ab32559)

Immunohistochemical staining of paraffin embedded rat spleen with purified ab32559 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-SHP1 antibody [Y476] (ab32559)

All lanes : Anti-SHP1 antibody [Y476] (ab32559) at 1/1000 dilution (purified)

Lane 1 : SP2/0 cell lysate
Lane 2 : mouse marrow
Lane 3 : rat brain
Lane 4 : C6 cell lysate
Lane 5 : rat cerebral cortex

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 68 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-SHP1 antibody [Y476] (ab32559)

All lanes : Anti-SHP1 antibody [Y476] (ab32559) at 1/1000 dilution (purified)

Lane 1 : A431 cell lysate
Lane 2 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 68 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] (ab32559)

Immunohistochemical staining of paraffin embedded mouse liver with purified ab32559 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-SHP1 antibody [Y476] (ab32559)

All lanes : Anti-SHP1 antibody [Y476] (ab32559) at 1/1000 dilution (unpurified)

Lane 1 : A- A431 cell lysate
Lane 2 : B- Jurkat cell lysate
Lane 3 : C- K562 cell lysate

Predicted band size: 68 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] (ab32559)

Unpurified ab32559, at a 1/50 dilution, staining human lymph node by immunohistochemistry, Paraffin embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] (ab32559)

Tissue Microarrays stained for "Anti-SHP1 antibody [Y476]” using "ab32559" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab32559 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHP1 antibody [Y476] (ab32559)

Tissue Microarrays stained for "Anti-SHP1 antibody [Y476]” using "ab32559" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab32559 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Anti-SHP1 antibody [Y476] (ab32559)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab32559? Please let us know so that we can cite the reference in this datasheet.

ab32559 has been referenced in 22 publications.

Dong Z et al. Hepatic Reduction in Cholesterol 25-Hydroxylase Aggravates Diet-induced Steatosis. Cell Mol Gastroenterol Hepatol 13:1161-1179 (2022). PubMed: 34990887
Tathe P et al. SHP-1 dephosphorylates histone H2B to facilitate its ubiquitination during transcription. EMBO J 41:e109720 (2022). PubMed: 35938192
Zhuang X et al. SHP-1 knockdown suppresses mitochondrial biogenesis and aggravates mitochondria-dependent apoptosis induced by all trans retinal through the STING/AMPK pathways. Mol Med 28:125 (2022). PubMed: 36273174
Chen Q et al. TRIM18-Regulated STAT3 Signaling Pathway via PTP1B Promotes Renal Epithelial-Mesenchymal Transition, Inflammation, and Fibrosis in Diabetic Kidney Disease. Front Physiol 12:709506 (2021). PubMed: 34434118
Bian Y et al. SMURF1-mediated ubiquitylation of SHP-1 promotes cell proliferation and invasion of endometrial stromal cells in endometriosis. Ann Transl Med 9:362 (2021). PubMed: 33842583

View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Application

Western blot

Sample

Rat Tissue lysate - whole (LIVER)

Gel Running Conditions

Reduced Denaturing

Loading amount

50 µg

Specification

LIVER

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 17 2016

Application

Western blot

Sample

Mouse Cell lysate - whole cell (CD4+ splenocytes)

Loading amount

100000 cells

Specification

CD4+ splenocytes

Treatment

anti-CD3 anti-CD28 antibody-coated beads

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 27 2006

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (22)

Customer reviews and Q&As

Western blot abreview for Anti-SHP1 antibody [Y476]

Western blot abreview for Anti-SHP1 antibody [Y476]

Post-translational
modifications