Anti-SHP1 (phospho S591) antibody (ab41436)
Key features and details
- Rabbit polyclonal to SHP1 (phospho S591)
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SHP1 (phospho S591) antibody
See all SHP1 primary antibodies -
Description
Rabbit polyclonal to SHP1 (phospho S591) -
Host species
Rabbit -
Specificity
Phospho-Ser591-SHP1 specific. This antibody detects a 68 kDa band on SDS-PAGE immunoblots of human Jurkat and A431 cells treated with pervanadate, as well as A431 cells treated with EGF. -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human SHP1 (phospho S591).
Database link: P29350 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was cross-adsorbed to a non specific phospho-serine peptide then affinity purified using phospho-Ser591-SHP1 peptide (immunogen). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
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| WB |
1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
WB
1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
Target
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Function
Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. -
Tissue specificity
Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain. -
Post-translational
modificationsPhosphorylated on serine and tyrosine residues. -
Cellular localization
Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II. - Information by UniProt
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Database links
- Entrez Gene: 5777 Human
- Entrez Gene: 15170 Mouse
- Entrez Gene: 116689 Rat
- Omim: 176883 Human
- SwissProt: P29350 Human
- SwissProt: P29351 Mouse
- SwissProt: P81718 Rat
- Unigene: 63489 Human
see all -
Alternative names
- 70Z-SHP antibody
- EC 3.1.3.48 antibody
- HCP antibody
see all
Images
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Western blot - Anti-SHP1 (phospho S591) antibody (ab41436)Lanes 1-2 : anti-SHP1 (C-terminal)
Lanes 3-6 : Anti-SHP1 (phospho S591) antibody (ab41436) at 1/1000 dilution
Lanes 1 & 3 : human Jurkat cells treated with 1 mM pervanadate for 30 min.
Lanes 2 & 4 : human Jurkat cells treated with 1 mM pervanadate for 30 min, blot exposed to lambda phosphatase before probing
Lane 5 : human Jurkat cells treated with 1 mM pervanadate for 30 min. with phospho-Ser591-SHP1 peptide
Lane 6 : human Jurkat cells treated with 1 mM pervanadate for 30 min. with non specific phospho-serine peptide
Predicted band size: 68 kDa
Observed band size: 68 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab41436 has been referenced in 1 publication.
- Mkaddem SB et al. Lyn and Fyn function as molecular switches that control immunoreceptors to direct homeostasis or inflammation. Nat Commun 8:246 (2017). PubMed: 28811476