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RecombinantRabMAb

Recombinant Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)

Reviews (3) Submit a question References (1)
Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)
  • Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)
  • Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)
  • Dot Blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)
  • Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19196] to SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163)
  • Suitable for: Dot blot, WB
  • Reacts with: Recombinant fragment

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196]

  • Description

    Rabbit monoclonal [EPR19196] to SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163)

  • Host species

    Rabbit

  • Specificity

    Expected to react with Snf1lk (when phosphorylated at T182) and SIK3 ((when phosphorylated at T163).

  • Tested applications

    Suitable for: Dot blot, WBmore details

  • Species reactivity

    Reacts with: Recombinant fragment
    Predicted to work with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with human Snf1lk or Snf1lk2 or SIK3 (WT) whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab199474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
1/1000.
WB (3)
1/1000. Detects a band of approximately 85, 104, 140 kDa.
Notes
Dot blot
1/1000.
WB
1/1000. Detects a band of approximately 85, 104, 140 kDa.

Target

Images

  • Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)
    Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)
    All lanes : Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with empty vector (control), whole cell lysate
    Lane 2 : HEK-293T transfected with a human SIK3 (WT) expression vector, whole cell lysate
    Lane 3 : HEK-293T transfected with a human SIK3 (WT) expression vector, then treated with alkaline phosphatase for 1 hour, whole cell lysate
    Lane 4 : HEK-293T transfected with a human SIK3 T163A mutant expression vector, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Observed band size: 140 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    The plasmids were kindly provided by our collaborator Dr. Yi Rao, Peking University.

  • Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)
    Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)
    All lanes : Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (control), whole cell lysate
    Lane 2 : HEK-293T transfected with a human SIK2 (WT) expression vector, whole cell lysate
    Lane 3 : HEK-293T transfected with a human SIK2 (WT) expression vector, then treated with alkaline phosphatase for 1 hour, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Observed band size: 104 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    The plasmids were kindly provided by our collaborator Dr. Yi Rao, Peking University.

  • Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)
    Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)
    All lanes : Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (control), whole cell lysate
    Lane 2 : HEK-293T transfected with a human SIK1 (WT) expression vector, whole cell lysate
    Lane 3 : HEK-293T transfected with a human SIK1 (WT) expression vector, then treated with alkaline phosphatase for 1 hour, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Observed band size: 85 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    The plasmids were kindly provided by our collaborator Dr. Yi Rao, Peking University.

  • Dot Blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)
    Dot Blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)

    Dot blot analysis of Snf1lk2 (phospho T175) labeled with ab199474 at 1/1,000 dilution.

    Lane 1: Snf1lk2 (phospho T175) peptide

    Lane 2: Snf1lk2 non-phospho peptide

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100,000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)
    Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab199474? Please let us know so that we can cite the reference in this datasheet.

ab199474 has been referenced in 1 publication.

  • Hu G  et al. High-throughput phenotypic screen and transcriptional analysis identify new compounds and targets for macrophage reprogramming. Nat Commun 12:773 (2021). PubMed: 33536439

Customer reviews and Q&As

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1-3 of 3 Abreviews

Western blot abreview for Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (PBMC)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
30 µg
Specification
PBMC
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Paul Donnelly

Verified customer

Submitted Sep 09 2022

Western blot abreview for Anti-Snf1lk (phospho T182) + Snf1lk2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (A549 cells (Wild Type and LKB1 addback))
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
30 µg
Specification
A549 cells (Wild Type and LKB1 addback)
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 05 2019

Western blot abreview for Anti-Snf1lk (phospho T182) + Snf1lk2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (HEK 293T)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
20 µg
Specification
HEK 293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. liu yuxiang

Verified customer

Submitted Nov 10 2017

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