Recombinant Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19196] to SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163)
- Suitable for: Dot blot, WB
- Reacts with: Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] -
Description
Rabbit monoclonal [EPR19196] to SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) -
Host species
Rabbit -
Specificity
Expected to react with Snf1lk (when phosphorylated at T182) and SIK3 ((when phosphorylated at T163). -
Tested applications
Suitable for: Dot blot, WBmore details -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with human Snf1lk or Snf1lk2 or SIK3 (WT) whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19196 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Dot blot |
1/1000.
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WB | (3) |
1/1000. Detects a band of approximately 85, 104, 140 kDa.
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Notes |
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Dot blot
1/1000. |
WB
1/1000. Detects a band of approximately 85, 104, 140 kDa. |
Target
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Cellular localization
Snf1lk: Cytoplasm. Nucleus. Translocates to the cytoplasm on phosphorylation where it binds binding to YWHAZ. Snf1lk2: Cytoplasm. SIK3: Cytoplasm -
Database links
- Entrez Gene: 150094 Human
- Entrez Gene: 23235 Human
- Entrez Gene: 23387 Human
- Omim: 605705 Human
- Omim: 608973 Human
- Omim: 614776 Human
- SwissProt: P57059 Human
- SwissProt: Q9H0K1 Human
see all
Images
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Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)All lanes : Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with empty vector (control), whole cell lysate
Lane 2 : HEK-293T transfected with a human SIK3 (WT) expression vector, whole cell lysate
Lane 3 : HEK-293T transfected with a human SIK3 (WT) expression vector, then treated with alkaline phosphatase for 1 hour, whole cell lysate
Lane 4 : HEK-293T transfected with a human SIK3 T163A mutant expression vector, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking and dilution buffer and concentration: 5% NFDM/TBST.
The plasmids were kindly provided by our collaborator Dr. Yi Rao, Peking University.
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Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)All lanes : Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (control), whole cell lysate
Lane 2 : HEK-293T transfected with a human SIK2 (WT) expression vector, whole cell lysate
Lane 3 : HEK-293T transfected with a human SIK2 (WT) expression vector, then treated with alkaline phosphatase for 1 hour, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 104 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking and dilution buffer and concentration: 5% NFDM/TBST.
The plasmids were kindly provided by our collaborator Dr. Yi Rao, Peking University.
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Western blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)All lanes : Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (control), whole cell lysate
Lane 2 : HEK-293T transfected with a human SIK1 (WT) expression vector, whole cell lysate
Lane 3 : HEK-293T transfected with a human SIK1 (WT) expression vector, then treated with alkaline phosphatase for 1 hour, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer and concentration: 5% NFDM/TBST.
The plasmids were kindly provided by our collaborator Dr. Yi Rao, Peking University.
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Dot Blot - Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) antibody [EPR19196] (ab199474)
Dot blot analysis of Snf1lk2 (phospho T175) labeled with ab199474 at 1/1,000 dilution.
Lane 1: Snf1lk2 (phospho T175) peptide
Lane 2: Snf1lk2 non-phospho peptide
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100,000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab199474 has been referenced in 1 publication.
- Hu G et al. High-throughput phenotypic screen and transcriptional analysis identify new compounds and targets for macrophage reprogramming. Nat Commun 12:773 (2021). PubMed: 33536439