Anti-SIRT3 antibody (ab189860)
Key features and details
- Rabbit polyclonal to SIRT3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SIRT3 antibody
See all SIRT3 primary antibodies -
Description
Rabbit polyclonal to SIRT3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human SIRT3 aa 1 to the C-terminus.
Database link: Q9NTG7 -
Positive control
- WB: HeLa cell extracts. IHC: human thyroid cancer, rat kidney, mouse kidney.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 44 kDa.
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| IHC-P | (1) |
1/20 - 1/50.
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| Notes |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 44 kDa. |
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IHC-P
1/20 - 1/50. |
Target
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Function
NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the sirtuin family. Class I subfamily.
Contains 1 deacetylase sirtuin-type domain. -
Post-translational
modificationsProcessed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 23410 Human
- Entrez Gene: 64384 Mouse
- Entrez Gene: 293615 Rat
- Omim: 604481 Human
- SwissProt: Q9NTG7 Human
- SwissProt: Q8R104 Mouse
- Unigene: 716456 Human
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Alternative names
- hSIRT 3 antibody
- hSIRT3 antibody
- Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody
see all
Images
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Western blot - Anti-SIRT3 antibody (ab189860)All lanes : Anti-SIRT3 antibody (ab189860) at 1/500 dilution
Lane 1 : HeLa cells extracts
Lane 2 : SIRT3 knockout (KO) HeLa cells extracts
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody (ab189860)Paraffin-embedded human thyroid cancer stained for SIRT3 using ab189860 at 1:100 dilution in immunohistochemical analysis.
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Western blot - Anti-SIRT3 antibody (ab189860)Anti-SIRT3 antibody (ab189860) at 1/1000 dilution + Extracts of SW620 cells at 25 µg
Secondary
HRP Goat Anti-Rabbit IgG
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 180 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody (ab189860)Paraffin-embedded rat kidney tissue stained for SIRT3 using ab189860 at 1:100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT3 antibody (ab189860)Paraffin-embedded mouse kidney tissue stained for SIRT3 using ab189860 at 1:100 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab189860 has been referenced in 8 publications.
- Diao Z et al. SIRT3 consolidates heterochromatin and counteracts senescence. Nucleic Acids Res 49:4203-4219 (2021). PubMed: 33706382
- Zheng K et al. Protective effects of sirtuin 3 on titanium particle-induced osteogenic inhibition by regulating the NLRP3 inflammasome via the GSK-3ß/ß-catenin signalling pathway. Bioact Mater 6:3343-3357 (2021). PubMed: 33817415
- Xiao L et al. Sustained Release of Melatonin from GelMA Liposomes Reduced Osteoblast Apoptosis and Improved Implant Osseointegration in Osteoporosis. Oxid Med Cell Longev 2020:6797154 (2020). PubMed: 32566094
- Naaz S et al. Activation of SIRT1/PGC 1a/SIRT3 pathway by melatonin provides protection against mitochondrial dysfunction in isoproterenol induced myocardial injury. Heliyon 6:e05159 (2020). PubMed: 33088945
- Rezzani R et al. A carnosine analog with therapeutic potentials in the treatment of disorders related to oxidative stress. PLoS One 14:e0215170 (2019). PubMed: 30964920
- Zhou W et al. Melatonin Increases Bone Mass around the Prostheses of OVX Rats by Ameliorating Mitochondrial Oxidative Stress via the SIRT3/SOD2 Signaling Pathway. Oxid Med Cell Longev 2019:4019619 (2019). PubMed: 31110599
- Sun L et al. Exosomal miRNA Let-7 from Menstrual Blood-Derived Endometrial Stem Cells Alleviates Pulmonary Fibrosis through Regulating Mitochondrial DNA Damage. Oxid Med Cell Longev 2019:4506303 (2019). PubMed: 31949877
- Gao J et al. Trilobatin Protects Against Oxidative Injury in Neuronal PC12 Cells Through Regulating Mitochondrial ROS Homeostasis Mediated by AMPK/Nrf2/Sirt3 Signaling Pathway. Front Mol Neurosci 11:267 (2018). PubMed: 30104959