Recombinant Anti-SIRT3 antibody [EPR23514-36] (ab246522)
![Western blot - Anti-SIRT3 antibody [EPR23514-36] (ab246522)](https://www.abcam.com/ps/products/246/ab246522/Images/ab246522-381963-AntiSIRT3-antibody-EPR2351436-western-blot-mouse-heart.jpg "Western blot - Anti-SIRT3 antibody [EPR23514-36] (ab246522)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23514-36] to SIRT3
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-SIRT3 antibody [EPR23514-36]
See all SIRT3 primary antibodies -
Description
Rabbit monoclonal [EPR23514-36] to SIRT3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF,IHC-Fr or IHC-P -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse heart, liver and kidney tissue lysate; Rat liver and kidney tissue lysate. IP: Mouse and rat liver lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23514-36 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab246522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 43 kDa.
|
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| IP |
1/30.
|
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 43 kDa. |
|
IP
1/30. |
Target
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Function
NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the sirtuin family. Class I subfamily.
Contains 1 deacetylase sirtuin-type domain. -
Post-translational
modificationsProcessed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 64384 Mouse
- Entrez Gene: 293615 Rat
- SwissProt: Q8R104 Mouse
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Alternative names
- hSIRT 3 antibody
- hSIRT3 antibody
- Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody
see all
Images
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Western blot - Anti-SIRT3 antibody [EPR23514-36] (ab246522)All lanes : Anti-SIRT3 antibody [EPR23514-36] (ab246522) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate 20 µg
Lane 2 : Mouse kidney tissue lysate 20 µg
Lane 3 : Mouse spleen tissue lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 43 kDaBlocking/diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Spleen (PMID:25566066).
Exposure time: 15 secs.
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![Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] (ab246522)](https://www.abcam.com/ps/products/246/ab246522/Images/ab246522-381967-AntiSIRT3-antibody-EPR2351436-immunoprecipitation-mouse-liver.jpg)
Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] (ab246522)SIRT3 was immunoprecipitated from 0.35 mg mouse liver lysate with ab246522 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246522 at a 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse liver lysate 10μg
Lane 2: ab246522 IP in mouse liver lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246522 in mouse liver lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 secs.
The target band size is very close to IgG light chain.
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![Western blot - Anti-SIRT3 antibody [EPR23514-36] (ab246522)](https://www.abcam.com/ps/products/246/ab246522/Images/ab246522-381965-AntiSIRT3-antibody-EPR2351436-western-blot-rat-liver.jpg)
Western blot - Anti-SIRT3 antibody [EPR23514-36] (ab246522)All lanes : Anti-SIRT3 antibody [EPR23514-36] (ab246522) at 1/1000 dilution
Lane 1 : Rat liver tissue lysate 20 µg
Lane 2 : Rat kidney tissue lysate 20 µg
Lane 3 : Rat spleen tissue lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?Blocking/diluting buffer and concentration: 5% NFDM/TBST
Negative control: Spleen (PMID:25566066).
Exposure time: 15 secs.
-
![Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] (ab246522)](https://www.abcam.com/ps/products/246/ab246522/Images/ab246522-381968-AntiSIRT3-antibody-EPR2351436-immunoprecipitation-rat-liver.jpg)
Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] (ab246522)SIRT3 was immunoprecipitated from 0.35 mg rat liver lysate with ab246522 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246522 at a 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat liver lysate 10μg
Lane 2: ab246522 IP in rat liver lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246522 in rat liver lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 32 secs.
The target band size is very close to IgG light chain.
-
![Western blot - Anti-SIRT3 antibody [EPR23514-36] (ab246522)](https://www.abcam.com/ps/products/246/ab246522/Images/ab246522-381966-AntiSIRT3-antibody-EPR2351436-western-blot-mouse-liver.jpg)
Western blot - Anti-SIRT3 antibody [EPR23514-36] (ab246522)Anti-SIRT3 antibody [EPR23514-36] (ab246522) at 1/1000 dilution + Mouse liver tissue lysate 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 43 kDaBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 secs.
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![Anti-SIRT3 antibody [EPR23514-36] (ab246522)](https://www.abcam.com/ps/products/246/ab246522/Images/ab246522-6-benefits-of-recombinant-antibodies.png)
Anti-SIRT3 antibody [EPR23514-36] (ab246522)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab246522 has been referenced in 1 publication.
- Zhu X et al. ANGPTL4 Attenuates Ang II-Induced Atrial Fibrillation and Fibrosis in Mice via PPAR Pathway. Cardiol Res Pract 2021:9935310 (2021). PubMed: 34422410