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RecombinantRabMAb

Recombinant Anti-SIRT7 antibody [EPR23811-15] (ab259968)

Submit a review Submit a question References (1)
Western blot - Anti-SIRT7 antibody [EPR23811-15] (ab259968)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] (ab259968)
  • Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] (ab259968)
  • Western blot - Anti-SIRT7 antibody [EPR23811-15] (ab259968)
  • Western blot - Anti-SIRT7 antibody [EPR23811-15] (ab259968)
  • Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] (ab259968)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] (ab259968)
  • Anti-SIRT7 antibody [EPR23811-15] (ab259968)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23811-15] to SIRT7
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-SIRT7 antibody [EPR23811-15]
    See all SIRT7 primary antibodies

  • Description

    Rabbit monoclonal [EPR23811-15] to SIRT7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Hepa1-6, Neuro-2a, NIH/3T3, RAW 264.7, C6, HeLa, HEK-293T, HepG2 and MCF7 whole cell lysates; Mouse spleen, skeletal muscle and liver tissue lysates; Ratskeletal muscle and liver tissue lysates. IHC-P: Mouse liver tissue; Rat spleen tissue. IP: HeLa and Neuro-2a whole cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab259968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/30.
WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
IP
1/30.
WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    NAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation.

  • Sequence similarities

    Belongs to the sirtuin family. Class IV subfamily.
    Contains 1 deacetylase sirtuin-type domain.

  • Post-translational
    modifications

    Phosphorylated during mitosis.

  • Cellular localization

    Cytoplasm. Nucleus, nucleolus. Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • NAD dependent deacetylase sirtuin 7 antibody
    • NAD dependent protein deacetylase sirtuin 7 antibody
    • NAD-dependent protein deacetylase sirtuin-7 antibody
    • Regulatory protein SIR2 homolog 7 antibody
    • Silent mating type information regulation 2 homolog antibody
    • Silent mating type information regulation 2 S.cerevisiae homolog 7 antibody
    • SIR2 L7 antibody
    • SIR2 like protein 7 antibody
    • Sir2 related protein type 7 antibody
    • SIR2, S.CEREVISIAE, HOMOLOG-LIKE 7 antibody
    • SIR2-like protein 7 antibody
    • SIR2L 7 antibody
    • SIR2L7 antibody
    • SIR7_HUMAN antibody
    • SIRT 7 antibody
    • SIRT7 antibody
    • Sirtuin 7 antibody
    • Sirtuin type 7 antibody
    • Sirtuin7 antibody
    see all

Images

  • Western blot - Anti-SIRT7 antibody [EPR23811-15] (ab259968)
    Western blot - Anti-SIRT7 antibody [EPR23811-15] (ab259968)
    All lanes : Anti-SIRT7 antibody [EPR23811-15] (ab259968) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] (ab259968)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] (ab259968)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SIRT7 with ab259968 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in mouse liver (PMID: 16618798). The section was incubated with ab259968 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] (ab259968)
    Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] (ab259968)

    SIRT7 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate with ab259968 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259968 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 ug

    Lane 2: ab259968 IP in Neuro-2a whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259968 in Neuro-2a whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 67 seconds.

     

  • Western blot - Anti-SIRT7 antibody [EPR23811-15] (ab259968)
    Western blot - Anti-SIRT7 antibody [EPR23811-15] (ab259968)
    All lanes : Anti-SIRT7 antibody [EPR23811-15] (ab259968) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse skeletal muscle tissue lysate
    Lane 3 : Rat spleen tissue lysate
    Lane 4 : Rat liver tissue lysate
    Lane 5 : Rat skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Low expression: skeletal muscle (PMID: 16618798).

  • Western blot - Anti-SIRT7 antibody [EPR23811-15] (ab259968)
    Western blot - Anti-SIRT7 antibody [EPR23811-15] (ab259968)
    All lanes : Anti-SIRT7 antibody [EPR23811-15] (ab259968) at 1/1000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate
    Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
    Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 5 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 6 : C6 (rat glial tumor glial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The expression pattern of SIRT7 is consistent with what has been described in the literature(PMID: 24771643, 16618798).

  • Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] (ab259968)
    Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] (ab259968)

    SIRT7 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate with ab259968 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259968 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug

    Lane 2: ab259968 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259968 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] (ab259968)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] (ab259968)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling SIRT7 with ab259968 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in rat spleen (PMID: 16618798). The section was incubated with ab259968 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Anti-SIRT7 antibody [EPR23811-15] (ab259968)
    Anti-SIRT7 antibody [EPR23811-15] (ab259968)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab259968? Please let us know so that we can cite the reference in this datasheet.

ab259968 has been referenced in 1 publication.

  • Yu AQ  et al. SIRT7-Induced PHF5A Decrotonylation Regulates Aging Progress Through Alternative Splicing-Mediated Downregulation of CDK2. Front Cell Dev Biol 9:710479 (2021). PubMed: 34604215

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