Recombinant Anti-SKP2 antibody [EPR3306(2)] (ab124799)
![Western blot - Anti-SKP2 antibody [EPR3306(2)] (ab124799)](https://www.abcam.com/ps/products/124/ab124799/Images/ab124799-365969-anti-skp2-antibody-epr33062-western-blot-knockout-hap1-skp2-hela-mcf7-human-lysates.jpg "Western blot - Anti-SKP2 antibody [EPR3306(2)] (ab124799)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3306(2)] to SKP2
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SKP2 antibody [EPR3306(2)]
See all SKP2 primary antibodies -
Description
Rabbit monoclonal [EPR3306(2)] to SKP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human SKP2 aa 150-250. The exact sequence is proprietary.
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Positive control
- 293T, HeLa, SW480 and LnCaP cell lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3306(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 46, 48 kDa (predicted molecular weight: 46, 48 kDa).
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| ICC/IF | (1) |
1/50 - 1/100.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 46, 48 kDa (predicted molecular weight: 46, 48 kDa). |
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ICC/IF
1/50 - 1/100. |
Target
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Function
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, MLL, CDK9, RAG2, FOXO1A, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 F-box domain.
Contains 9 LRR (leucine-rich) repeats. - Information by UniProt
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Database links
- Entrez Gene: 6502 Human
- Omim: 601436 Human
- SwissProt: Q13309 Human
- Unigene: 23348 Human
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Alternative names
- CDK2/Cyclin A associated protein p45 antibody
- Cyclin A/CDK2 associated protein p45 antibody
- Cyclin-A/CDK2-associated protein p45 antibody
see all
Images
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Western blot - Anti-SKP2 antibody [EPR3306(2)] (ab124799)All lanes : Anti-SKP2 antibody [EPR3306(2)] (ab124799) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : SKP2 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 46, 48 kDaLanes 1 - 4: Merged signal (red and green). Green - ab124799 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab124799 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in SKP2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SKP2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab124799 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-SKP2 antibody [EPR3306(2)] (ab124799)](https://www.abcam.com/ps/products/124/ab124799/Images/ab124799-138694-anti-skp2-antibody-epr33062-western-blot.jpg)
Western blot - Anti-SKP2 antibody [EPR3306(2)] (ab124799)All lanes : Anti-SKP2 antibody [EPR3306(2)] (ab124799) at 1/1000 dilution
Lane 1 : 293T cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : SW480 cell lysates
Lane 4 : LnCaP cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46, 48 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SKP2 antibody [EPR3306(2)] (ab124799)](https://www.abcam.com/ps/products/124/ab124799/Images/ab124799-138693-anti-skp2-antibody-epr33062-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SKP2 antibody [EPR3306(2)] (ab124799)ab124799, at a 1/50 dilution, staining SKP2 in HeLa cells by Immunofluorescence. -
![Anti-SKP2 antibody [EPR3306(2)] (ab124799)](https://www.abcam.com/ps/products/124/ab124799/Images/ab124799-4-benefits-of-recombinant-antibodies.png)
Anti-SKP2 antibody [EPR3306(2)] (ab124799)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab124799 has been referenced in 1 publication.
- Yang B et al. H2A.Z regulates tumorigenesis, metastasis and sensitivity to cisplatin in intrahepatic cholangiocarcinoma. Int J Oncol 52:1235-1245 (2018). PubMed: 29532867