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Anti-SLC14A2 antibody (ab95365)

Reviews (1)Q&A (1)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC14A2 antibody (ab95365)
  • Western blot - Anti-SLC14A2 antibody (ab95365)

Key features and details

  • Rabbit polyclonal to SLC14A2
  • Suitable for: WB, IHC-P
  • Reacts with: Rat
  • Isotype: IgG

Overview

  • Product name

    Anti-SLC14A2 antibody
    See all SLC14A2 primary antibodies

  • Description

    Rabbit polyclonal to SLC14A2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Human

  • Immunogen

    Synthetic peptide corresponding to the C terminal amino acids 911-929 (

    QEKNRRASMITKYQAYDVS

    ) of Rat SLC14A2 (NP_062220).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Rat colon tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab95365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 102 kDa. 1 µg/ml of ab95365 was sufficient for detection of SLC14A2 in 20µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
IHC-P
1/25.
Notes
WB
1/1000. Predicted molecular weight: 102 kDa. 1 µg/ml of ab95365 was sufficient for detection of SLC14A2 in 20µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
IHC-P
1/25.

Target

  • Function

    Specialized low-affinity vasopressin-regulated urea transporter. Mediates rapid transepithelial urea transport across the inner medullary collecting duct and plays a major role in the urinary concentrating mechanism.

  • Tissue specificity

    Isoform 1 and isoform 2 are expressed in the inner medulla of the kidney.

  • Sequence similarities

    Belongs to the urea transporter family.

  • Cellular localization

    Apical cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • FLJ16167 antibody
      • hUT-A6 antibody
      • HUT2 antibody
      • kidney antibody
      • MGC119566 antibody
      • MGC119567 antibody
      • Slc14a2 antibody
      • Solute carrier family 14 (urea transporter), member 2 antibody
      • Solute carrier family 14 member 2 antibody
      • Urea transporter 2 antibody
      • Urea transporter antibody
      • Urea transporter, kidney antibody
      • UT-A2 antibody
      • UT2 antibody
      • UT2_HUMAN antibody
      • UTA antibody
      • UTR antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC14A2 antibody (ab95365)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC14A2 antibody (ab95365)

      Immunohistochemical analysis of paraffin-embedded formalin fixed (4%) rat kidney tissue labelling SLC14A2 with ab95365 at 1/25 dilution for 1 hour at RT, followed by secondary antibody Goat Anti-Rabbit IgG (Alexa Fluor 488) (Green). Membrane staining on rat kidney tissue is observed. Counter stained with Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 (Red) F-Actin stain. (C) UT-A1 Antibody (D) Composite image.

    • Western blot - Anti-SLC14A2 antibody (ab95365)
      Western blot - Anti-SLC14A2 antibody (ab95365)
      Western blot detection of SLC14A2 in Rat inner medulla using ab95365 at 1:1000 dilution.

    References (1)

    Publishing research using ab95365? Please let us know so that we can cite the reference in this datasheet.

    ab95365 has been referenced in 1 publication.

    • Laszczyk AM  et al. Pax2 and Pax8 Proteins Regulate Urea Transporters and Aquaporins to Control Urine Concentration in the Adult Kidney. J Am Soc Nephrol 31:1212-1225 (2020). PubMed: 32381599

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-SLC14A2 antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Kidney)
    Specification
    Kidney
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 28 2022

    Question

    Inquiry: Greetings. I will be teaching a Molecular Biology laboratory class at XXXXX, Spring of 2013. This is a 5 credit class, app 16 students. We will be removing five organs from mice, extracting the RNA, DNA, and proteins from the organ tissue, and subjecting these macromolecules PCR, in vitro transcription, in vitro translation, and immunoblotting techniques. Which marker proteins and antibodies would you suggest for the following organ tissues: liver, brain, kidney, testes, ovary. for use for immunoblotting detection?

    Read More
    Answer

    Thank you for contacting Abcam.

    I've compiled a list of primary antibodies that have been tested in mouse tissue via western blot. The only ovary-specific antibodies that I found were anti-human (with only 50% homology with the respective mouse protein), so the ovary antibody listed below is not exclusively-specific to ovary but also gives a positive signal in kidney, placenta, and small intestine:

    1. Liver: anti-albumin ab135575 (https://www.abcam.com/albumin-antibody-c-terminal-ab135575.html)
    2. Brain: anti-neuron specific beta III tubulin ab18207 (https://www.abcam.com/neuron-specific-beta-iii-tubulin-antibody-neuronal-marker-ab18207.html)
    3. Kidney: anti-SLC14A2 ab95365 (https://www.abcam.com/slc14a2-antibody-ab95365.html)
    4. Testis: anti-histone H2B ab23913 (https://www.abcam.com/histone-h2b-testis-specific-antibody-ab23913.html)
    5. Ovary: anti-ENPP2 (also kidney, placenta, small intestine): ab77104 (https://www.abcam.com/enpp2-antibody-5h3-ab77104.html)

    The advantage of this combination of antibodies is that you can use the same anti-rabbit secondary since they were all raised in a rabbit host. Our anti-rabbit secondary antibodies are listed in the link below:
    https://www.abcam.com/Search?Keywords=anti-rabbit&fReset=1&pt=-1&source=TopSearch

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link:
    https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

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