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    products/primary-antibodies/slc14a2-antibody-epr8620-ab156589.pdf

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Signal Transduction Metabolism Plasma Membrane Channels
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RecombinantRabMAb

Recombinant Anti-SLC14A2 antibody [EPR8620] (ab156589)

  • Datasheet
  • SDS
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Western blot - Anti-SLC14A2 antibody [EPR8620] (ab156589)
  • Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] (ab156589)
  • Anti-SLC14A2 antibody [EPR8620] (ab156589)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8620] to SLC14A2
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Primary
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Anti-ALP antibody (ab154100)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-SLC14A2 antibody [EPR8620]
    See all SLC14A2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8620] to SLC14A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SLC14A2 aa 500-600. The exact sequence is proprietary.
    Database link: Q15849

  • Positive control

    • K562, Human fetal kidney, 293T and TF1 lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8620
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels
    • Neuroscience
    • Neurology process
    • Neuroendocrinology
    • Oxytocin & Vasopressin

Associated products

  • Alternative Versions

    • Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (ab249277)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • K-562 whole cell lysate (ab7911)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab156589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 101 kDa.
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 101 kDa.
IP
1/10 - 1/100.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Target

  • Function

    Specialized low-affinity vasopressin-regulated urea transporter. Mediates rapid transepithelial urea transport across the inner medullary collecting duct and plays a major role in the urinary concentrating mechanism.
  • Tissue specificity

    Isoform 1 and isoform 2 are expressed in the inner medulla of the kidney.
  • Sequence similarities

    Belongs to the urea transporter family.
  • Cellular localization

    Apical cell membrane.
  • Target information above from: UniProt accession Q15849 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8170 Human
    • Omim: 601611 Human
    • SwissProt: Q15849 Human
    • Unigene: 710927 Human
    • Alternative names

      • FLJ16167 antibody
      • hUT-A6 antibody
      • HUT2 antibody
      • kidney antibody
      • MGC119566 antibody
      • MGC119567 antibody
      • Slc14a2 antibody
      • Solute carrier family 14 (urea transporter), member 2 antibody
      • Solute carrier family 14 member 2 antibody
      • Urea transporter 2 antibody
      • Urea transporter antibody
      • Urea transporter, kidney antibody
      • UT-A2 antibody
      • UT2 antibody
      • UT2_HUMAN antibody
      • UTA antibody
      • UTR antibody
      see all

    Images

    • Western blot - Anti-SLC14A2 antibody [EPR8620] (ab156589)
      Western blot - Anti-SLC14A2 antibody [EPR8620] (ab156589)
      All lanes : Anti-SLC14A2 antibody [EPR8620] (ab156589) at 1/1000 dilution

      Lane 1 : K562 lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : 293T lysate
      Lane 4 : TF1 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 101 kDa

    • Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] (ab156589)
      Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] (ab156589)
      Detection of SLC14A2 by Western Blot of Immunprecipitate.
      293T cell lysate pellet, immunoprecipitated using ab156589 at 1/10 dilution.
    • Anti-SLC14A2 antibody [EPR8620] (ab156589)
      Anti-SLC14A2 antibody [EPR8620] (ab156589)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab156589? Please let us know so that we can cite the reference in this datasheet.

    ab156589 has been referenced in 1 publication.

    • Serman Y  et al. Emerging new role of NFAT5 in inducible nitric oxide synthase in response to hypoxia in mouse embryonic fibroblast cells. Am J Physiol Cell Physiol 317:C31-C38 (2019). PubMed: 31067085

    Customer reviews and Q&As

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