Recombinant Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (ab249277)
![Western blot - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (ab249277)](https://www.abcam.com/ps/products/249/ab249277/Images/ab249277-402529-anti-slc14a2-antibody-epr8620-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (ab249277)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8620] to SLC14A2 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free
See all SLC14A2 primary antibodies -
Description
Rabbit monoclonal [EPR8620] to SLC14A2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249277 is the carrier-free version of ab156589.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8620 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 101 kDa.
|
| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 101 kDa. |
Target
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Function
Specialized low-affinity vasopressin-regulated urea transporter. Mediates rapid transepithelial urea transport across the inner medullary collecting duct and plays a major role in the urinary concentrating mechanism. -
Tissue specificity
Isoform 1 and isoform 2 are expressed in the inner medulla of the kidney. -
Sequence similarities
Belongs to the urea transporter family. -
Cellular localization
Apical cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8170 Human
- Omim: 601611 Human
- SwissProt: Q15849 Human
- Unigene: 710927 Human
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Alternative names
- FLJ16167 antibody
- hUT-A6 antibody
- HUT2 antibody
see all
Images
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Western blot - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (ab249277)All lanes : Anti-SLC14A2 antibody [EPR8620] (ab156589) at 1/1000 dilution
Lane 1 : K562 lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : 293T lysate
Lane 4 : TF1 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 101 kDaThis data was developed using ab156589, the same antibody clone in a different buffer formulation.
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![Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (ab249277)](https://www.abcam.com/ps/products/249/ab249277/Images/ab249277-1-anti-slc14a2-antibody-epr8620-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (ab249277) -
![Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (ab249277)](https://www.abcam.com/ps/products/249/ab249277/Images/ab249277-3-benefits-of-recombinant-antibodies.png)
Anti-SLC14A2 antibody [EPR8620] - BSA and Azide free (ab249277)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249277 has not yet been referenced specifically in any publications.
