Recombinant Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)
![Western blot - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)](https://www.abcam.com/ps/products/124/ab124826/Images/ab124826-138768-anti-slc22a3-emt-antibody-epr6630-western-blot.jpg "Western blot - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6630] to SLC22A3/OCT3
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SLC22A3/OCT3 antibody [EPR6630]
See all SLC22A3/OCT3 primary antibodies -
Description
Rabbit monoclonal [EPR6630] to SLC22A3/OCT3 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SLC22A3/OCT3 aa 300-400. The exact sequence is proprietary.
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Positive control
- Human fetal kidney, Human muscle and Human placenta lysates; Human kidney tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.75 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6630 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 61 kDa).
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 80 kDa (predicted molecular weight: 61 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Mediates potential-dependent transport of a variety of organic cations. May play a significant role in the disposition of cationic neurotoxins and neurotransmitters in the brain. -
Tissue specificity
Expressed in placenta, skeletal muscle, prostate, aorta, liver, fetal lung, salivary gland, adrenal gland, kidney and brain cortex. No expression detected in spleen. -
Sequence similarities
Belongs to the major facilitator superfamily. Organic cation transporter family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6581 Human
- Omim: 604842 Human
- SwissProt: O75751 Human
- Unigene: 567337 Human
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Alternative names
- EMT antibody
- EMT organic cation transporter 3 antibody
- EMTH antibody
see all
Images
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Western blot - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)All lanes : Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826) at 1/10000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human muscle tissue lysate
Lane 3 : Human placenta tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 61 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)](https://www.abcam.com/ps/products/124/ab124826/Images/ab124826-208128-anti-slc22a3-emt-antibody-epr6630-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)Overlay histogram showing JEG3 cells stained with ab124826 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124826, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)](https://www.abcam.com/ps/products/124/ab124826/Images/ab124826-138767-anti-slc22a3-emt-antibody-epr6630-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)ab124826, at 1/100 dilution, staining SLC22A3/OCT3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)](https://www.abcam.com/ps/products/124/ab124826/Images/ab124826-138766-anti-slc22a3-emt-antibody-epr6630.jpg)
OI-RD Scanning - Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826) -
![Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)](https://www.abcam.com/ps/products/124/ab124826/Images/ab124826-5-benefits-of-recombinant-antibodies.png)
Anti-SLC22A3/OCT3 antibody [EPR6630] (ab124826)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab124826 has been referenced in 10 publications.
- Vachalova V et al. Functional reorganization of monoamine transport systems during villous trophoblast differentiation: evidence of distinct differences between primary human trophoblasts and BeWo cells. Reprod Biol Endocrinol 20:112 (2022). PubMed: 35927731
- Cervenkova L et al. Protein expression of ABCC2 and SLC22A3 associates with prognosis of pancreatic adenocarcinoma. Sci Rep 9:19782 (2019). PubMed: 31874997
- Gu J et al. Upregulated OCT3 has the potential to improve the survival of colorectal cancer patients treated with (m)FOLFOX6 adjuvant chemotherapy. Int J Colorectal Dis 34:2151-2159 (2019). PubMed: 31732877
- Guttmann S et al. Organic cation transporter 3 mediates cisplatin and copper cross-resistance in hepatoma cells. Oncotarget 9:743-754 (2018). PubMed: 29416650
- Irannejad R et al. Functional selectivity of GPCR-directed drug action through location bias. Nat Chem Biol 13:799-806 (2017). PubMed: 28553949
- Le Roy B et al. Assessment of the Relation between the Expression of Oxaliplatin Transporters in Colorectal Cancer and Response to FOLFOX-4 Adjuvant Chemotherapy: A Case Control Study. PLoS One 11:e0148739 (2016). PubMed: 26859833
- Gai Z et al. Organic Cation Transporter 2 Overexpression May Confer an Increased Risk of Gentamicin-Induced Nephrotoxicity. Antimicrob Agents Chemother 60:5573-80 (2016). PubMed: 27401566
- Oguri T et al. Organic cation transporter 6 directly confers resistance to anticancer platinum drugs. Biomed Rep 5:639-643 (2016). WB ; Human . PubMed: 27882231
- Aschauer L et al. Expression of xenobiotic transporters in the human renal proximal tubule cell line RPTEC/TERT1. Toxicol In Vitro N/A:N/A (2014). PubMed: 25500123
- Patel H et al. Differential expression of organic cation transporter OCT-3 in oral premalignant and malignant lesions: potential implications in the antineoplastic effects of metformin. J Oral Pathol Med : (2012). PubMed: 22861817