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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-SLC27A4 / FATP4 antibody [EPR17319] - BSA and Azide free (ab271965)

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Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - BSA and Azide free (ab271965)
  • Immunocytochemistry/ Immunofluorescence - Anti-SLC27A4 / FATP4 antibody [EPR17319] - BSA and Azide free (ab271965)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC27A4 / FATP4 antibody [EPR17319] - BSA and Azide free (ab271965)
  • Immunoprecipitation - Anti-SLC27A4 / FATP4 antibody [EPR17319] - BSA and Azide free (ab271965)
  • Anti-SLC27A4 / FATP4 antibody [EPR17319] - BSA and Azide free (ab271965)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17319] to SLC27A4 / FATP4 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-SLC27A4 / FATP4 antibody [EPR17319] - BSA and Azide free
    See all SLC27A4 / FATP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR17319] to SLC27A4 / FATP4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK293 and HepG2 whole cell lysates. Human fetal brain and Human fetal kidney lysates. IHC-P: Human kidney tissue. ICC/IF: HepG2 cells. IP: HEK293 whole cell lysate.

  • General notes

    ab271965 is the carrier-free version of ab199719.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

Target

  • Relevance

    SLC27A4 / FATP4 plays a role in the transport of long chain fatty acids across the plasma membrane. It has acyl-coA ligase activity for long chain and very long chain fatty acids. Deletion of the SLC27A4 gene results in embryonic lethality, which is attributed to the need for fat absorption across the visceral endoderm early in embryonic development. Expression of FAT4P in the intestinal lining is thought to be altered in response to dietary fat.

  • Cellular localization

    Cell Membrane

  • Database links

    • Alternative names

      • ACSVL 4 antibody
      • ACSVL4 antibody
      • EC 6.2.1 antibody
      • FATP 4 antibody
      • FATP4 antibody
      • Fatty acid transport protein 4 antibody
      • Fatty acid transport protein4 antibody
      • IPS antibody
      • Long chain fatty acid transport protein 4 antibody
      • Long chain fatty acid transport protein4 antibody
      • OTTHUMP00000022264 antibody
      • S27A4 antibody
      • SLC27 A4 antibody
      • SLC27A 4 antibody
      • Solute carrier family 27 (fatty acid transporter) member 4 antibody
      • Solute carrier family 27 member 4 antibody
      • Solute carrier family 27 member4 antibody
      see all

    Images

    • Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - BSA and Azide free (ab271965)
      Western blot - Anti-SLC27A4 / FATP4 antibody [EPR17319] - BSA and Azide free (ab271965)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: SLC27A4/FATP4 knockout HAP1 cell lysate (20 µg) 
      Lane 3: HEK293 cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab199719 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab199719 was shown to specifically react with SLC27A4/FATP4 when SLC27A4/FATP4 knockout samples were used. Wild-type and SLC27A4/FATP4 knockout samples were subjected to SDS-PAGE. ab199719 and ab8245 (loading control to GAPDH) were diluted 1/2000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199719).

    • Immunocytochemistry/ Immunofluorescence - Anti-SLC27A4 / FATP4 antibody [EPR17319] - BSA and Azide free (ab271965)
      Immunocytochemistry/ Immunofluorescence - Anti-SLC27A4 / FATP4 antibody [EPR17319] - BSA and Azide free (ab271965)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling SLC27A4 / FATP4 with ab199719 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HepG2 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows:-
      -ve control 1: ab199719 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199719).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC27A4 / FATP4 antibody [EPR17319] - BSA and Azide free (ab271965)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC27A4 / FATP4 antibody [EPR17319] - BSA and Azide free (ab271965)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SLC27A4 / FATP4 with ab199719 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human kidney tissue is observed. Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199719).
    • Immunoprecipitation - Anti-SLC27A4 / FATP4 antibody [EPR17319] - BSA and Azide free (ab271965)
      Immunoprecipitation - Anti-SLC27A4 / FATP4 antibody [EPR17319] - BSA and Azide free (ab271965)

      SLC27A4 / FATP4 was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab199719 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab199719 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Lane 1: HEK293  whole cell lysate 10µg (Input). Lane 2: HEK293 whole cell lysate following IP. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199719 in HEK293 whole cell lysate.


      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure is 3 minutes.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199719).
    • Anti-SLC27A4 / FATP4 antibody [EPR17319] - BSA and Azide free (ab271965)
      Anti-SLC27A4 / FATP4 antibody [EPR17319] - BSA and Azide free (ab271965)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab271965? Please let us know so that we can cite the reference in this datasheet.

    ab271965 has not yet been referenced specifically in any publications.

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