Recombinant Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
![Western blot - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)](https://www.abcam.com/ps/products/180/ab180516/Images/ab180516-420476-anti-slc6a19-antibody-epr14154-b-westernblot-human-mouse-rat.jpg "Western blot - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14154(B)] to SLC6A19
- Suitable for: WB, Indirect ELISA
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-SLC6A19 antibody [EPR14154(B)]
See all SLC6A19 primary antibodies -
Description
Rabbit monoclonal [EPR14154(B)] to SLC6A19 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Indirect ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human SLC6A19 aa 350-450 (internal sequence). The exact sequence is proprietary.
Database link: Q695T7 -
Positive control
- WB: Caco-2, HeLa whole cell lysates, Human and mouse kidney lysates, mouse and rat brain lysates and mouse and rat heart lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14154(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 71 kDa.
For unpurified use at 1/1000 - 1/10000. |
|
| Indirect ELISA |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 71 kDa. For unpurified use at 1/1000 - 1/10000. |
|
Indirect ELISA
Use at an assay dependent concentration. |
Target
-
Relevance
Transporter that mediates epithelial resorption of neutral amino acids across the apical membrane of epithelial cells in the kidney and intestine. It appears that leucine is the preferred substrate, but all large neutral non-aromatic L-amino acids bind to this transporter. Uptake of leucine is sodium-dependent. In contrast to other members of the neurotransmitter transporter family, does not appear to be chloride-dependent -
Database links
- Entrez Gene: 340024 Human
- SwissProt: Q695T7 Human
-
Alternative names
- B(0)AT1 antibody
- Sodium dependent neutral amino acid transporter B(0) antibody
- Solute carrier family 6 member 19 antibody
- System B(0) neutral amino acid transporter antibody
Images
-
Western blot - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)All lanes : Anti-SLC6A19 antibody [EPR14154(B)] (ab180516) at 1/1000 dilution (Purified)
Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Human kidney lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Mouse brain lysate
Lane 6 : Rat brain lysate
Lane 7 : Mouse heart lysate
Lane 8 : Rat heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 71 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in the literature (PMID: 25884559).
Blocking Buffer and concentration: 5% NFDM/TBST
-
![Indirect ELISA - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)](https://www.abcam.com/ps/products/180/ab180516/Images/ab180516-11-anti-slc6a19-antibody-epr14154-elisa-human-slc6a19.jpg)
Indirect ELISA - Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)ELISA analysis of Human SLC6A19 recombinant protein at 500 ng/mL with ab180516. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
-
![Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)](https://www.abcam.com/ps/products/180/ab180516/Images/ab180516-10-benefits-of-recombinant-antibodies.png)
Anti-SLC6A19 antibody [EPR14154(B)] (ab180516)
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (5)
ab180516 has been referenced in 5 publications.
- Zhang Q et al. ACE2 interaction with cytoplasmic PDZ protein enhances SARS-CoV-2 invasion. iScience 24:102770 (2021). PubMed: 34189428
- Williams TL et al. Human embryonic stem cell-derived cardiomyocyte platform screens inhibitors of SARS-CoV-2 infection. Commun Biol 4:926 (2021). PubMed: 34326460
- Yang X et al. Effects of chronic stress on intestinal amino acid pathways. Physiol Behav 204:199-209 (2019). PubMed: 30831184
- Butts M et al. Moderate Alcohol Consumption Inhibits Sodium-Dependent Glutamine Co-Transport in Rat Intestinal Epithelial Cells in Vitro and Ex Vivo. Nutrients 11:N/A (2019). PubMed: 31635319
- Zhao Y et al. SLC6A1-miR133a-CDX2 loop regulates SK-OV-3 ovarian cancer cell proliferation, migration and invasion. Oncol Lett 16:4977-4983 (2018). PubMed: 30250563