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Anti-SLIRP antibody [1E10BC8] (ab119687)

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Western blot - Anti-SLIRP antibody [1E10BC8] (ab119687)
  • Immunocytochemistry/ Immunofluorescence - Anti-SLIRP antibody [1E10BC8] (ab119687)
  • Flow Cytometry - Anti-SLIRP antibody [1E10BC8] (ab119687)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLIRP antibody [1E10BC8] (ab119687)

Key features and details

  • Mouse monoclonal [1E10BC8] to SLIRP
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-SLIRP antibody [1E10BC8]
    See all SLIRP primary antibodies

  • Description

    Mouse monoclonal [1E10BC8] to SLIRP

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab119687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 12 kDa.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 5 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 12 kDa.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 
ICC/IF
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 5 µg/ml.

Target

  • Relevance

    SLIRP is a RNA binding protein that acts as a nuclear receptor corepressor. It may act by binding the SRA RNA, and repressing the SRA-mediated nuclear receptor coactivation. SLIRP binds the STR7 loop of SRA RNA. It is also able to repress glucocorticoid (GR), androgen (AR), thyroid (TR) and VDR-mediated transactivation. It contains 1 RRM (RNA recognition motif) domain. SLIRP is predominantly founs in mitochondrial with a limited role in the nucleus where it is recruited to nuclear receptor target promoters.

  • Cellular localization

    Mitochondrial and Nuclear; Predominantly mitochondrial.

  • Database links

    • Alternative names

      • C14orf156 antibody
      • Chromosome 14 open reading frame 156 antibody
      • DC50 antibody
      • PD04872 antibody
      • SLIRP antibody
      • SRA stem loop interacting RNA binding protein, mitochondrial antibody
      see all

    Images

    • Western blot - Anti-SLIRP antibody [1E10BC8] (ab119687)
      Western blot - Anti-SLIRP antibody [1E10BC8] (ab119687)
      All lanes : Anti-SLIRP antibody [1E10BC8] (ab119687) at 1 µg/ml

      Lane 1 : HepG2 lysate at 20 µg
      Lane 2 : fibroblast lysate at 20 µg/ml
      Lane 3 : SLIRP recombinant protein at 0.01 µg

      Secondary
      All lanes : Infra red fluorescence - Goat anti-mouse 800 at 1/5000 dilution

      Predicted band size: 12 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SLIRP antibody [1E10BC8] (ab119687)
      Immunocytochemistry/ Immunofluorescence - Anti-SLIRP antibody [1E10BC8] (ab119687)
      Immunocytochemistry of ab119687 stained HDFn cells (human). The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were then incubated with the antibody (ab119687, 1µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondria.
    • Flow Cytometry - Anti-SLIRP antibody [1E10BC8] (ab119687)
      Flow Cytometry - Anti-SLIRP antibody [1E10BC8] (ab119687)
      Fibroblast (HDFn) cells were stained with 1µg/mL SLIRP antibody (ab119687) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLIRP antibody [1E10BC8] (ab119687)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLIRP antibody [1E10BC8] (ab119687)

      IHC image of SLIRP staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119687, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References (0)

    Publishing research using ab119687? Please let us know so that we can cite the reference in this datasheet.

    ab119687 has not yet been referenced specifically in any publications.

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