Anti-SLUG antibody (ab27568)
Key features and details
- Rabbit polyclonal to SLUG
- Suitable for: WB, IHC-Fr
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG
Overview
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Product name
Anti-SLUG antibody
See all SLUG primary antibodies -
Description
Rabbit polyclonal to SLUG -
Host species
Rabbit -
Specificity
ab27568 detects the overexpressing human Slug protein, however we have no data on the detection of the endogenous Human protein with this current batch. ab27568 does not cross react with overexpressed SNAIL protein in cell lysates. -
Tested applications
Suitable for: WB, IHC-Frmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Human
Predicted to work with: Cow, Dog, Pig, Xenopus laevis -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
1/500 - 1/1000. Detects a band of approximately 30-34 kDa (predicted molecular weight: 30 kDa).
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IHC-Fr | (2) |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 30-34 kDa (predicted molecular weight: 30 kDa). |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
Transcriptional repressor. Involved in the generation and migration of neural crest cells. -
Tissue specificity
Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. -
Involvement in disease
Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. -
Sequence similarities
Belongs to the snail C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 432368 Chicken
- Entrez Gene: 6591 Human
- Entrez Gene: 20583 Mouse
- Entrez Gene: 25554 Rat
- Entrez Gene: 379440 Xenopus laevis
- Omim: 602150 Human
- SwissProt: Q3MHQ4 Cow
- SwissProt: O43623 Human
see all -
Alternative names
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
- Protein snail homolog 2 antibody
see all
Images
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Transverse section showing Slug-expressing neural crest cells (green) in the dorsal neural tube (NT) in the hindbrain of a quail embryo. 4% PFA fixed embryonic day 1.7 Japanese Quail embryo, frozen in Tissutek, sectioned 12 um. Ab27568 1/400, incubated overnight. Goat anti-rabbit: Alexa488 (Molecular Probes) 1/1000 incubated for 2 hours.
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All lanes : Anti-SLUG antibody (ab27568) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Heart (Rat) Tissue Lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDaSecondary antibody - goat anti-rabbit HRP (preadsorbed)
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All lanes : Anti-SLUG antibody (ab27568) at 1/1000 dilution
Lane 1 : MCF7 cell lysate overexpressing SLUG protein
Lane 2 : MCF7 lysate overexpressing SNAIL protein
Lane 3 : MCF7 lysate tranfected with vector control plasmid
Secondary
All lanes : HRP-conjugated anti-rabbit antibody at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
ab27568 detects a band of 34 kDa in MCF7 cells that have been transfected with a vector that overexpresses the SLUG protein. This antibody does not crossreact with the SNAIL protein, as it did not recognize this protein in MCF7 cells overexpressing SNAIL. ab27568 was unable to detect endogenous SLUG in MDA-MB231 cells (not shown).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (142)
ab27568 has been referenced in 142 publications.
- Lin Q et al. Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling. Exp Ther Med 23:3 (2022). PubMed: 34815755
- Huang CS et al. Long Noncoding RNA LINC02470 Sponges MicroRNA-143-3p and Enhances SMAD3-Mediated Epithelial-to-Mesenchymal Transition to Promote the Aggressive Properties of Bladder Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 35205713
- Yu Y et al. Synergistic effect of Tripterygium glycosides and cisplatin on drug-resistant human epithelial ovarian cancer via ILK/GSK3β/Slug signal pathway. Am J Transl Res 14:2051-2062 (2022). PubMed: 35422913
- Wang LN et al. TGF-β1/SH2B3 axis regulates anoikis resistance and EMT of lung cancer cells by modulating JAK2/STAT3 and SHP2/Grb2 signaling pathways. Cell Death Dis 13:472 (2022). PubMed: 35589677
- Nam HJ et al. GPR110 promotes progression and metastasis of triple-negative breast cancer. Cell Death Discov 8:271 (2022). PubMed: 35614051