Recombinant Anti-SLUG antibody [EPR25113-46] (ab302780)
![Western blot - Anti-SLUG antibody [EPR25113-46] (AB302780)](https://www.abcam.com/ps/products/302/ab302780/Images/AB302780-490604-anti-slug-antibody-epr25113-46-western-blot-a204.jpg "Western blot - Anti-SLUG antibody [EPR25113-46] (AB302780)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25113-46] to SLUG
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-SLUG antibody [EPR25113-46]
See all SLUG primary antibodies -
Description
Rabbit monoclonal [EPR25113-46] to SLUG -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra),IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A-204 whole cell lysate. His-tagged human SLUG recombinant protein.
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General notes
AB302780 does not react in Western blot in mouse and rat samples.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25113-46 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab302780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa). |
Target
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Function
Transcriptional repressor. Involved in the generation and migration of neural crest cells. -
Tissue specificity
Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. -
Involvement in disease
Defects in SNAI2 are the cause of Waardenburg syndrome type 2D (WS2D) [MIM:608890]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1. -
Sequence similarities
Belongs to the snail C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6591 Human
- Omim: 602150 Human
- SwissProt: O43623 Human
- Unigene: 360174 Human
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Alternative names
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
- Protein snail homolog 2 antibody
see all
Images
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Western blot - Anti-SLUG antibody [EPR25113-46] (AB302780)All lanes : Anti-SLUG antibody [EPR25113-46] (ab302780) at 1/1000 dilution
Lane 1 : A-204 (Human muscle rhabdomyosarcoma), whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 48 secondsBlocking and dilution buffer: 5% NFDM/TBST.
Negative control: MCF7 (PMID:19756381).
This blot was developed using a high sensitivity ECL substrate.
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![Western blot - Anti-SLUG antibody [EPR25113-46] (AB302780)](https://www.abcam.com/ps/products/302/ab302780/Images/AB302780-490605-anti-slug-antibody-epr25113-46-western-blot-human-slug.jpg)
Western blot - Anti-SLUG antibody [EPR25113-46] (AB302780)All lanes : Anti-SLUG antibody [EPR25113-46] (ab302780) at 1/1000 dilution
Lane 1 : His-tagged human SLUG recombinant protein, 10 ng
Lane 2 : His-tagged human SNAI1 recombinant protein, 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
This antibody does not cross-react with human SNAI1.
Both recombinant proteins were made in-house and expressed from the E. coli expression systems.
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![Anti-SLUG antibody [EPR25113-46] (AB302780)](https://www.abcam.com/ps/products/302/ab302780/Images/AB302780-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-SLUG antibody [EPR25113-46] (AB302780)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab302780 has not yet been referenced specifically in any publications.