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Anti-Smac/Diablo antibody [8H5AA3] (ab110288)

Submit a review Submit a question References (1)
Flow Cytometry - Anti-Smac / Diablo antibody [8H5AA3] (ab110288)
  • Immunocytochemistry - Anti-Smac / Diablo antibody [8H5AA3] (ab110288)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac / Diablo antibody [8H5AA3] (ab110288)

Key features and details

  • Mouse monoclonal [8H5AA3] to Smac/Diablo
  • Suitable for: ICC, Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Smac/Diablo antibody [8H5AA3]
    See all Smac/Diablo primary antibodies

  • Description

    Mouse monoclonal [8H5AA3] to Smac/Diablo

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • Human HDFn cells and HeLa cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal testis.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use a concentration of 1 µg/ml.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
IHC-P
Use a concentration of 5 µg/ml.
Notes
ICC
Use a concentration of 1 µg/ml.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
IHC-P
Use a concentration of 5 µg/ml.

Target

  • Function

    Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).

  • Tissue specificity

    Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes.

  • Domain

    The mature N-terminus mediates interaction with XIAP.

  • Cellular localization

    Mitochondrion. Released into the cytosol when cells undergo apoptosis.
  • Information by UniProt

  • Database links

    • Alternative names

      • 0610041G12Rik antibody
      • DBLOH_HUMAN antibody
      • DBOH antibody
      • DFNA64 antibody
      • diablo antibody
      • Diablo homolog (Drosophila) antibody
      • Diablo homolog antibody
      • Diablo homolog mitochondrial antibody
      • Diablo IAP binding mitochondrial protein antibody
      • Diablo like protein antibody
      • DIABLO S antibody
      • Direct IAP binding protein with low pI antibody
      • Direct IAP-binding protein with low pI antibody
      • FLJ10537 antibody
      • FLJ25049 antibody
      • mitochondrial antibody
      • Mitochondrial Smac protein antibody
      • Second mitochondria derived activator of caspase antibody
      • Second mitochondria-derived activator of caspase antibody
      • second mitochondrial activator of caspases antibody
      • SMAC 3 antibody
      • Smac antibody
      • Smac protein antibody
      • SMAC3 antibody
      see all

    Images

    • Flow Cytometry - Anti-Smac / Diablo antibody [8H5AA3] (ab110288)
      Flow Cytometry - Anti-Smac / Diablo antibody [8H5AA3] (ab110288)
      HeLa cells were stained with 1 µg/mL Smac / Diablo antibody (ab110288) (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
    • Immunocytochemistry - Anti-Smac / Diablo antibody [8H5AA3] (ab110288)
      Immunocytochemistry - Anti-Smac / Diablo antibody [8H5AA3] (ab110288)
      Immunocytochemistry image of Smac / Diablo stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with the antibody (ab110288) at 1µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac / Diablo antibody [8H5AA3] (ab110288)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac / Diablo antibody [8H5AA3] (ab110288)

      IHC image of Smac / Diablo staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab110288, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References (1)

    Publishing research using ab110288? Please let us know so that we can cite the reference in this datasheet.

    ab110288 has been referenced in 1 publication.

    • Wang C  et al. Bone morphogenetic protein-2 exhibits therapeutic benefits for osteonecrosis of the femoral head through induction of cartilage and bone cells. Exp Ther Med 15:4298-4308 (2018). PubMed: 29849774

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