Anti-Smac/Diablo antibody [8H5AB2] (ab110291)
![Western blot - Anti-Smac / Diablo antibody [8H5AB2] (ab110291)](https://www.abcam.com/ps/products/110/ab110291/Images/Smac-Diablo-Primary-antibodies-ab110291-2.gif "Western blot - Anti-Smac / Diablo antibody [8H5AB2] (ab110291)")
Key features and details
- Mouse monoclonal [8H5AB2] to Smac/Diablo
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2
Overview
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Product name
Anti-Smac/Diablo antibody [8H5AB2]
See all Smac/Diablo primary antibodies -
Description
Mouse monoclonal [8H5AB2] to Smac/Diablo -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is considered to be commercially sensitive.
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Positive control
- Human liver mitochondria and HepG2 cells
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
8H5AB2 -
Isotype
IgG2 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa.
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| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa. |
Target
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Function
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). -
Tissue specificity
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. -
Domain
The mature N-terminus mediates interaction with XIAP. -
Cellular localization
Mitochondrion. Released into the cytosol when cells undergo apoptosis. - Information by UniProt
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Database links
- Entrez Gene: 56616 Human
- Omim: 605219 Human
- SwissProt: Q9NR28 Human
- Unigene: 169611 Human
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Alternative names
- 0610041G12Rik antibody
- DBLOH_HUMAN antibody
- DBOH antibody
see all
Images
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Western blot - Anti-Smac / Diablo antibody [8H5AB2] (ab110291)All lanes : Anti-Smac/Diablo antibody [8H5AB2] (ab110291) at 1 µg/ml
Lane 1 : Human liver mitichondria
Lane 2 : HepG2 cells
Predicted band size: 27 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab110291 has not yet been referenced specifically in any publications.