Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451)
Key features and details
- Rabbit polyclonal to SMAD1/3/5 (phospho S423 + S425)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-SMAD1/3/5 (phospho S423 + S425) antibody -
Description
Rabbit polyclonal to SMAD1/3/5 (phospho S423 + S425) -
Host species
Rabbit -
Specificity
ab51451 is specific for the phosphorylated form of the protein although minimal reactivity with nonphosphorylated human Smad3 can be observed by ELISA and WB. ab51451 also reacts with phosphorylated Smad1 and Smad5 but negligible reactivity is seen against other phosphorylated Smad family members. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Xenopus laevis, Zebrafish
-
Immunogen
Synthetic peptide corresponding to Human SMAD1/3/5 aa 400-500 (phospho S423 + S425).
Database link: P84022 -
Positive control
- WB: HEK293, HeLa, MCF-7, Jurkat, A431, A549, LNCap, MOLT-4, Ramos, Raji, A-172, NIH/3T3 Lysate; MEF lysate; Hep3B lysat.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.33% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab51451 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (6) |
Use at an assay dependent concentration.
|
| WB | (3) |
1/2000 - 1/20000. Predicted molecular weight: 48 kDa. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested.
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
WB
1/2000 - 1/20000. Predicted molecular weight: 48 kDa. Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested. |
Target
-
Database links
- SwissProt: P84022 Human
- SwissProt: Q15797 Human
- SwissProt: Q99717 Human
-
Alternative names
- BSP-1 antibody
- BSP1 antibody
- DKFZP586N0721 antibody
see all
Images
-
Western blot - Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451)All lanes : Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451) at 1 µg/ml
Lane 1 : HEK293 lysate
Lane 2 : HeLa Lysate
Lane 3 : MCF-7 Lysate
Lane 4 : Jurkat Lysate
Lane 5 : A431 Lysate
Lane 6 : A549 Lysate
Lane 7 : LNCap Lysate
Lane 8 : MOLT-4 Lysate
Lane 9 : Ramos Lysate
Lane 10 : Raji Lysate
Lane 11 : A-172 Lysate
Lane 12 : NIH/3T3 Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 & 4-12 : Peroxidase rabbit secondary antibody at 1/30000 dilution
Lane 3 : Peroxidase rabbit secondary antibody
Predicted band size: 48 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451)ab51451 at 2.5 µg/ml staining epidermal keratinocytes at 40X. This image shows strong nuclear staining with localization of the antibody as the precipated red siganl and a hematoxylin purple nuclear counterstain.
-
Western blot - Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451)All lanes : Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451) at 1/2000 dilution
Lane 1 : MEF lysate (control)
Lane 2 : MEF lysate, after stimulation with 2 ng/ml of TGF-ß for 1 hour
Lane 3 : MEF-/-Smad3 lysate (control)
Lane 4 : MEF-/-Smad3 lysate, after stimulation with 2 ng/ml of TGF-ß for one hour
Lane 5 : Hep3B lysate (control)
Lane 6 : Hep3B lysate, after stimulation with 2 ng/ml of TGF-ß for one hour
Lane 7 : HEK 293 lysate (control)
Predicted band size: 48 kDa
Image courtesy of Ying Zhang, NIH, CCR, Bethesda, MD.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (52)
ab51451 has been referenced in 52 publications.
- Baik JE et al. TGF-β1 mediates pathologic changes of secondary lymphedema by promoting fibrosis and inflammation. Clin Transl Med 12:e758 (2022). PubMed: 35652284
- Walewska E et al. The Interaction Between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum. Front Endocrinol (Lausanne) 10:667 (2019). PubMed: 31632347
- Ursoli Ferreira F et al. Endothelial Cells Tissue-Specific Origins Affects Their Responsiveness to TGF-ß2 during Endothelial-to-Mesenchymal Transition. Int J Mol Sci 20:N/A (2019). PubMed: 30678183
- Riemondy KA et al. Single cell RNA sequencing identifies TGFß as a key regenerative cue following LPS-induced lung injury. JCI Insight 5:N/A (2019). PubMed: 30913038
- Chung YH et al. BMP-2 restoration aids in recovery from liver fibrosis by attenuating TGF-ß1 signaling. Lab Invest N/A:N/A (2018). WB ; Mouse . PubMed: 29789683