Anti-Smad2 + Smad3 (phospho T8) antibody (ab272332)
Key features and details
- Rabbit polyclonal to Smad2 + Smad3 (phospho T8)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Smad2 + Smad3 (phospho T8) antibody
See all Smad2 + Smad3 primary antibodies -
Description
Rabbit polyclonal to Smad2 + Smad3 (phospho T8) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Human Smad2 + Smad3 (phospho T8). Synthetic phosphopeptide derived from human Smad2/3 around the phosphorylation site of Threonine 8. Q15796,P84022.
Database link: P84022 -
Positive control
- WB: HEK293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/2000.
|
| Notes |
|---|
|
WB
1/1000 - 1/2000. |
Target
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Relevance
SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. -
Cellular localization
Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. -
Database links
- Entrez Gene: 4087 Human
- Entrez Gene: 4088 Human
- Entrez Gene: 17126 Mouse
- Entrez Gene: 17127 Mouse
- Entrez Gene: 25631 Rat
- Entrez Gene: 29357 Rat
- Omim: 601366 Human
- Omim: 603109 Human
see all -
Alternative names
- hMAD 2 antibody
- hMAD 3 antibody
- hSMAD2 antibody
see all
Images
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Western blot - Anti-Smad2 + Smad3 (phospho T8) antibody (ab272332)All lanes : Anti-Smad2 + Smad3 (phospho T8) antibody (ab272332) at 1/500 dilution
Lane 1 : HEK293T whole cell lysate
Lane 2 : HEK293T treated with TNFa(20ng/ml)for 10 min whole cell lysate
Lane 3 : Mouse Heart tissue lysate
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab272332 has been referenced in 5 publications.
- Zhu B et al. Piezo 1 activation facilitates cholangiocarcinoma metastasis via Hippo/YAP signaling axis. Mol Ther Nucleic Acids 24:241-252 (2021). PubMed: 33767919
- Shen K et al. Acetyl oxygen benzoate engeletin ester promotes KLF4 degradation leading to the attenuation of pulmonary fibrosis via inhibiting TGFß1-smad/p38MAPK-lnc865/lnc556-miR-29b-2-5p-STAT3 signal pathway. Aging (Albany NY) 13:13807-13821 (2021). PubMed: 33929970
- Zhu Z et al. NAT10 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Mediating N4-Acetylcytidine Modification of Gremlin 1. Stem Cells Int 2021:8833527 (2021). PubMed: 33953754
- Wang Y et al. miR-29b enhances the proliferation and migration of bone marrow mesenchymal stem cells in rats with castration-induced osteoporosis through the PI3K/AKT and TGF-ß/Smad signaling pathways. Exp Ther Med 20:3185-3195 (2020). PubMed: 32855687
- Zong M et al. Irbesartan ameliorates myocardial fibrosis in diabetic cardiomyopathy rats by inhibiting the TGFß1/Smad2/3 pathway. Exp Ther Med 20:117 (2020). PubMed: 33005243