Name: Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free
Brand: RabMAb
SKU: ab280897
Rating: 4 (1 reviews)

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Western blot - Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free (ab280897)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23681-40] to Smad2 (phospho S467) - BSA and Azide free
Suitable for: IHC-P, IP, Flow Cyt (Intra), Dot blot, WB, ICC/IF
Knockout validated
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 647 Unconjugated

Product name

Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free
See all Smad2 primary antibodies
Description

Rabbit monoclonal [EPR23681-40] to Smad2 (phospho S467) - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, IP, Flow Cyt (Intra), Dot blot, WB, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Wild-type HeLa treated/untreated with 20 ng/ml TGF beta1. NIH/3T3 treated/untreated with 20ng/ml TGF beta1 and 50 ÂµM MG-132. IHC-P: Human stomach tissue. Human colon carcinoma tissue. Mouse cardiac muscle. Rat lung tissue. ICC/IF: HeLa and NIH/3T3 cells treated with TGF beta1. Flow Cyt (intra): HeLa and NIH/3T3 cells treated with TGF beta1. IP: HeLa and NIH/3T3 cells treated with TGF beta1.
General notes
ab280897 is the carrier-free version of ab280888.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C.
Storage buffer

Constituent: 100% PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23681-40
Isotype

IgG
Research areas

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab280897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P	(1)	Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP		Use at an assay dependent concentration.
Flow Cyt (Intra)		Use at an assay dependent concentration.
Dot blot		Use at an assay dependent concentration.
WB		Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
ICC/IF		Use at an assay dependent concentration.

Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
Flow Cyt (Intra) Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
ICC/IF Use at an assay dependent concentration.

Function

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma.
Tissue specificity

Expressed at high levels in skeletal muscle, heart and placenta.
Sequence similarities

Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
Post-translational
modifications

Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation.
Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
Cellular localization

Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.
Target information above from: UniProt accession Q15796 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4087 Human
- Entrez Gene: 17126 Mouse
- Entrez Gene: 29357 Rat
- Omim: 601366 Human
- SwissProt: Q15796 Human
- SwissProt: Q62432 Mouse
- SwissProt: O70436 Rat
- Unigene: 12253 Human
- Unigene: 705764 Human
- Unigene: 391091 Mouse
- Unigene: 2755 Rat
see all
Alternative names
- Drosophila, homolog of, MADR2 antibody
- hMAD-2 antibody
- HsMAD2 antibody
- JV18 antibody
- JV18-1 antibody
- JV181 antibody
- MAD antibody
- MAD homolog 2 antibody
- MAD Related Protein 2 antibody
- Mad-related protein 2 antibody
- MADH2 antibody
- MADR2 antibody
- MGC22139 antibody
- MGC34440 antibody
- Mother against DPP homolog 2 antibody
- Mothers against decapentaplegic homolog 2 antibody
- Mothers against decapentaplegic, Drosophila, homolog of, 2 antibody
- Mothers against DPP homolog 2 antibody
- OTTHUMP00000163489 antibody
- Sma and Mad related protein 2 antibody
- Sma- and Mad-related protein 2 MAD antibody
- SMAD 2 antibody
- SMAD family member 2 antibody
- SMAD, mothers against DPP homolog 2 antibody
- SMAD2 antibody
- SMAD2_HUMAN antibody
see all

Western blot - Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free (ab280897)

All lanes : Anti-Smad2 (phospho S467) antibody [EPR23681-40] (ab280888) at 1/1000 dilution

Lane 1 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell) treated with 20 ng/ml TGF beta1 for 15 minutes, whole cell lysate
Lane 3 : Smad2 knockout HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : Smad2 knockout HeLa (human cervix adenocarcinoma epithelial cell), treated with 20 ng/ml TGF beta1 for 15 minutes, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?

This data was developed using ab280888, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS

Lanes 1-4: Merged signal (red and green). Green - ab280888 observed at 60 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

ab280888 Anti-Smad2 (phospho S467) antibody [EPR23681-40] was shown to specifically react with Smad2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255430 (knockout cell lysate (ab263833) was used. Wild-type and Smad2 knockout samples were subjected to SDS-PAGE.

ab280888 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Dot Blot - Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free (ab280897)

This data was developed using ab280888, the same antibody clone in a different buffer formulation.

Dot blot analysis of Smad2 (phospho S467) using ab280888 at 1:1000 (0.524 µg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

Lane 1: Smad2 peptide (aa 458-467)
Lane 2: Smad2 (phospho S465) peptide (aa 458-467)
Lane 3: Smad2 (phospho aa S465/S467) peptide (aa 458-467)
Lane 4: Smad2 (phospho S467) peptide (aa 458-467)

Exposure time: 3 minutes

Blocking and diluting buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free (ab280897)

This data was developed using ab280888, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human stomach tissue labelling Smad2 (phospho S467) with ab280888 at 1/2000 (0.262 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human stomach without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab280888 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free (ab280897)

This data was developed using ab280888, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labelling Smad2 (phospho S467) with ab280888 at 1/50 (10.48 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

Confocal image showing mainly nuclear staining in HeLa cells treated with TGF-β1 (20 ng/ml) for 15 mins.

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free (ab280897)

This data was developed using ab280888, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) treated with 20ng/ml TGF beta1 for 15 minutes (Red)/ Untreated control (Green) cells labelling Smad2 (phospho S467) with ab280888 at 1/5000 dilution (0.01µg)/ red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

The up-regulation of pSmad2 (S467) is induced by TGF-beta1 treatment in HeLa (PMID:24959295).
Immunoprecipitation - Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free (ab280897)

This data was developed using ab280888, the same antibody clone in a different buffer formulation.

Smad2 (phospho S467) was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) treated with 20ng/ml TGF beta1 for 15 minutes, whole cell lysate 10 µg with ab280888 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280888 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: HeLa treated with 20ng/ml TGF beta1 for 15 minutes, whole cell lysate 10 µg

Lane 2: ab280888 IP in HeLa treated with 20ng/ml TGF beta1 for 15 minutes whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab280888 in HeLa treated with 20ng/ml TGF beta1 for 15 minutes whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 15 seconds.
Western blot - Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free (ab280897)

All lanes : Anti-Smad2 (phospho S467) antibody [EPR23681-40] (ab280888) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) treated with 20ng/ml TGF beta1 for 15 minutes, whole cell lysate at 10 µg
Lane 2 : HeLa treated with 20ng/ml TGF beta1 for 15 minutes whole cell lysate (phosphatase treated membrane) at 10 µg
Lane 3 : HeLa whole cell lysate at 20 µg
Lane 4 : HeLa treated with 20ng/ml TGF beta1 for 15 minutes, whole cell lysate at 20 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?

This data was developed using ab280888, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature. (PMID: 24959295)

The up-regulation of pSmad2 (S467) is induced by TGF-beta1 treatment in HeLa (PMID:24959295).

Exposure time: 3 minutes.
Western blot - Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free (ab280897)

All lanes : Anti-Smad2 (phospho S467) antibody [EPR23681-40] (ab280888) at 1/1000 dilution

Lane 1 : NIH/3T3 (mouse embryonic fibroblast) treated with 20ng/ml TGF beta1 and 50 µM MG-132 for 15 minutes, whole cell lysate
Lane 2 : NIH/3T3 treated with 20ng/ml TGF beta1 and 50 µM MG-132 for 15 minutes whole cell lysate (phosphatase treated membrane)
Lane 3 : NIH/3T3 whole cell lysate
Lane 4 : NIH/3T3 treated with 20ng/ml TGF beta1 and 50 µM MG-132 for 15 minutes, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?

This data was developed using ab280888, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature. (PMID: 24347165)

The up-regulation of pSmad2 (S467) is induced by TGF-beta1 treatment in NIH/3T3 (PMID: 24347165)

Exposure time: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free (ab280897)

This data was developed using ab280888, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labelling Smad2 (phospho S467) with ab280888 at 1/2000 (0.262 µg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human colon carcinoma without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab280888 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free (ab280897)

This data was developed using ab280888, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labelling Smad2 (phospho S467) with ab280888 at 1/2000 (0.262 µg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse cardiac muscle without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab280888 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free (ab280897)

This data was developed using ab280888, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat lung tissue labelling Smad2 (phospho S467) with ab280888 at 1/2000 (0.262 µg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat lung without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab280888 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free (ab280897)

This data was developed using ab280888, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labelling Smad2 (phospho S467) with ab280888 at 1/50 (10.48 µg/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

Confocal image showing mainly nuclear staining in NIH/3T3 cells treated with TGF-β1 (20 ng/ml) for 15 mins.

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Flow Cytometry (Intracellular) - Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free (ab280897)

This data was developed using ab280888, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) treated with 20ng/ml TGF beta1 for 15 minutes (Red)/ Untreated control (Green) cells labelling Smad2 (phospho S467) with ab280888 at 1/5000 dilution (0.01µg)/ red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

The up-regulation of pSmad2 (S467) is induced by TGF-beta1 treatment in NIH/3T3 (PMID: 24347165).
Immunoprecipitation - Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free (ab280897)

This data was developed using ab280888, the same antibody clone in a different buffer formulation.

Smad2 (phospho S467) was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) treated with 20ng/ml TGF beta1 50 µM MG-132 for 15 minutes, whole cell lysate 10 µg with ab280888 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280888 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: NIH/3T3 treated with 20ng/ml TGF beta1 50 µM MG-132 for 15 minutes, whole cell lysate 10 µg

Lane 2: ab280888 IP in NIH/3T3 treated with 20ng/mltreated with 20ng/ml TGF beta1 50 µM MG-132 for 15 minutes, whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab280888 in NIH/3T3 treated with 20ng/mltreated with 20ng/ml TGF beta1 50 µM MG-132 for 15 minutes, whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 32 seconds.
Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free (ab280897)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab280897? Please let us know so that we can cite the reference in this datasheet.

ab280897 has not yet been referenced specifically in any publications.

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Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Appendix)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH 9.2

Permeabilization

Yes - Tween-20

Specification

Appendix

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Jul 12 2022

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Carrier free

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

Certificate of Compliance

References (0)

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Smad2 (phospho S467) antibody [EPR23681-40] - BSA and Azide free

Post-translational
modifications