Recombinant Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
![Western blot - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)](https://www.abcam.com/ps/products/245/ab245222/Images/ab245222-412773-anti-smarcd1-antibody-epr23170-71-western-blot-human-knockout-lysate.jpg "Western blot - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23170-71] to SMARCD1
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-SMARCD1 antibody [EPR23170-71]
See all SMARCD1 primary antibodies -
Description
Rabbit monoclonal [EPR23170-71] to SMARCD1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, 293T, NIH/3T3 and Neuro-2a lysates. ICC/IF: HeLa and Neuro-2a cells. Flow Cyt (intra): HeLa and NIH/3T3 cells. IP: HeLa and NIH/3T3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23170-71 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
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| WB |
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
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| ICC/IF |
1/50.
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| IP |
1/30.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/500. |
|
WB
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). |
|
ICC/IF
1/50. |
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IP
1/30. |
Target
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Function
Involved in chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Has a strong influence on the Vitamin D-mediated transcriptional activity from an enhancer Vitamin D receptor element (VDRE). May be a link between mammalian SWI-SNF-like chromatin remodeling complexes and the vitamin D receptor (VDR) heterodimer. Mediates critical interactions between nuclear receptors and the BRG1/SMARCA4 chromatin-remodeling complex for transactivation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. -
Tissue specificity
Expressed in all tissues tested, including brain, heart, kidney, liver, lung, muscle, pancreas and placenta. -
Sequence similarities
Belongs to the SMARCD family.
Contains 1 SWIB domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6602 Human
- Entrez Gene: 83797 Mouse
- Omim: 601735 Human
- SwissProt: Q96GM5 Human
- SwissProt: Q61466 Mouse
- Unigene: 79335 Human
- Unigene: 273756 Mouse
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Alternative names
- 60 kDa BRG-1/Brm-associated factor subunit A antibody
- 60 kDa BRG1/Brm associated factor subunit A antibody
- BAF60A antibody
see all
Images
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Western blot - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)All lanes : Anti-SMARCD1 antibody [EPR23170-71] (ab245222) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : SMARCD1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDaLanes 1-4: Merged signal (red and green). Green - ab245222 observed at 58 kDa. Red - loading control ab8245 observed at 36 kDa.
ab245222 Anti-SMARCD1 antibody [EPR23170-71] was shown to specifically react with SMARCD1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266458 (knockout cell lysate ab259143) was used. Wild-type and SMARCD1 knockout samples were subjected to SDS-PAGE. ab245222 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)](https://www.abcam.com/ps/products/245/ab245222/Images/ab245222-2-anti-smarcd1-antibody-epr23170-71-immunocytochemistry-neuro2a-mouse.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling SMARCD1 with ab245222 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in Neuro-2a cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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![Immunoprecipitation - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)](https://www.abcam.com/ps/products/245/ab245222/Images/ab245222-368561-anti-smarcd1-antibody-epr23170-71-immunoprecipitation-nih3t3-mouse.jpg)
Immunoprecipitation - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)SMARCD1 was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate with ab245222 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245222 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: NIH/3T3 whole cell lysate 10 (Input).
Lane 2: ab245222 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245222 in NIH/3T3 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes. -
![Flow Cytometry (Intracellular) - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)](https://www.abcam.com/ps/products/245/ab245222/Images/ab245222-3-anti-smarcd1-antibody-epr23170-71-flowcytometry-hela-human.jpg)
Flow Cytometry (Intracellular) - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling SMARCD1 with ab245222 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)](https://www.abcam.com/ps/products/245/ab245222/Images/ab245222-5-antismarcd1-antibody-epr23170-71-western-blot.jpg)
Western blot - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)All lanes : Anti-SMARCD1 antibody [EPR23170-71] (ab245222) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg
Lane 4 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Immunoprecipitation - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)](https://www.abcam.com/ps/products/245/ab245222/Images/ab245222-368560-anti-smarcd1-antibody-epr23170-71-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)SMARCD1 was immunoprecipitated from 0.35 mg of HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab245222 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245222 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 (Input).
Lane 2: ab245222 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245222 in HeLa whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes. -
![Flow Cytometry (Intracellular) - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)](https://www.abcam.com/ps/products/245/ab245222/Images/ab245222-4-anti-smarcd1-antibody-epr23170-71-flowcytometry-nih-3t3-mouse.jpg)
Flow Cytometry (Intracellular) - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling SMARCD1 with ab245222 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)](https://www.abcam.com/ps/products/245/ab245222/Images/ab245222-1-anti-smarcd1-antibody-epr23170-71-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SMARCD1 antibody [EPR23170-71] (ab245222)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling SMARCD1 with ab245222 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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![Anti-SMARCD1 antibody [EPR23170-71] (ab245222)](https://www.abcam.com/ps/products/245/ab245222/Images/ab245222-9-benefits-of-recombinant-antibodies.png)
Anti-SMARCD1 antibody [EPR23170-71] (ab245222)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab245222 has not yet been referenced specifically in any publications.