Anti-SMC2 antibody (ab10399)
Key features and details
- Rabbit polyclonal to SMC2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMC2 antibody
See all SMC2 primary antibodies -
Description
Rabbit polyclonal to SMC2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human SMC2 aa 50-150. The epitope maps to a region between residues 50 and 100 of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592).
Database link: O95347 -
Positive control
- WB: Mock transfected or SMC2 transfected HEK-293T whole cell lysates. IHC-P: Human cerebral cortex tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/5000 - 1/20000. Detects a band of approximately 150 kDa (predicted molecular weight: 143 kDa).
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/5000 - 1/20000. Detects a band of approximately 150 kDa (predicted molecular weight: 143 kDa). |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. -
Sequence similarities
Belongs to the SMC family. SMC2 subfamily. -
Domain
The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC4, forming a V-shaped heterodimer. -
Cellular localization
Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase. - Information by UniProt
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Database links
- Entrez Gene: 10592 Human
- Entrez Gene: 397800 Xenopus laevis
- Omim: 605576 Human
- SwissProt: O95347 Human
- SwissProt: P50533 Xenopus laevis
- Unigene: 119023 Human
- Unigene: 928 Xenopus laevis
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Alternative names
- CAP E antibody
- CAPE antibody
- Chromosome associated protein E antibody
see all
Images
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Western blot - Anti-SMC2 antibody (ab10399)All lanes : Anti-SMC2 antibody (ab10399) at 0.1 µg/ml
Lane 1 : E: Mock transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : T: SMC2 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 90 µg per lane.
Predicted band size: 143 kDa
Exposure time: 10 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC2 antibody (ab10399)IHC image of ab10399 staining in normal human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10399, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab10399 has been referenced in 8 publications.
- Baergen AK et al. Diminished Condensin Gene Expression Drives Chromosome Instability That May Contribute to Colorectal Cancer Pathogenesis. Cancers (Basel) 11:N/A (2019). PubMed: 31357676
- Poser E et al. Aurora A promotes chromosome congression by activating the condensin-dependent pool of KIF4A. J Cell Biol 219:N/A (2019). PubMed: 31881080
- Shirali AS et al. A multi-step transcriptional cascade underlies vascular regeneration in vivo. Sci Rep 8:5430 (2018). PubMed: 29615716
- Wang D et al. Hydrogen gas inhibits lung cancer progression through targeting SMC3. Biomed Pharmacother 104:788-797 (2018). WB . PubMed: 29852353
- Gerasimova T et al. A structural hierarchy mediated by multiple nuclear factors establishes IgH locus conformation. Genes Dev 29:1683-95 (2015). ChIP ; Mouse . PubMed: 26302788
- Pan X et al. YY1 controls Ig? repertoire and B-cell development, and localizes with condensin on the Ig? locus. EMBO J 32:1168-82 (2013). PubMed: 23531880
- Hancock R Structure of metaphase chromosomes: a role for effects of macromolecular crowding. PLoS One 7:e36045 (2012). ICC/IF . PubMed: 22540018
- Fazzio TG & Panning B Condensin complexes regulate mitotic progression and interphase chromatin structure in embryonic stem cells. J Cell Biol 188:491-503 (2010). WB ; Mouse . PubMed: 20176923