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    products/primary-antibodies/smc4-antibody-epr13366-c-terminal-ab179831.pdf

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Cell Biology Cell Cycle Cell Division Chromatid Cohesion
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RecombinantRabMAb

Recombinant Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)

  • Datasheet
  • SDS
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Western blot - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)
  • Immunocytochemistry/ Immunofluorescence - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)
  • Flow Cytometry (Intracellular) - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)
  • Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13366] to SMC4 - C-terminal
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Primary
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Anti-SMC2 antibody (ab10399)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-SMC4 antibody [EPR13366] - C-terminal
    See all SMC4 primary antibodies
  • Description

    Rabbit monoclonal [EPR13366] to SMC4 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Ramos, 293T, HeLa and Raji cell lysates. HeLa and Raji cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13366
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion

Associated products

  • Alternative Versions

    • Anti-SMC4 antibody [EPR13366] - BSA and Azide free (ab250130)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • Ramos whole cell lysate (ab3955)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Predicted molecular weight: 147 kDa.
ICC/IF
1/50 - 1/100.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/10000. Predicted molecular weight: 147 kDa.
ICC/IF
1/50 - 1/100.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
  • Tissue specificity

    Widely expressed. Higher expression in testis, colon, thymus.
  • Sequence similarities

    Belongs to the SMC family. SMC4 subfamily.
  • Domain

    The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC2, forming a V-shaped heterodimer.
  • Cellular localization

    Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
  • Target information above from: UniProt accession Q9NTJ3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10051 Human
    • Entrez Gene: 70099 Mouse
    • Omim: 605575 Human
    • SwissProt: Q9NTJ3 Human
    • SwissProt: Q8CG47 Mouse
    • Unigene: 58992 Human
    • Unigene: 206841 Mouse
    • Alternative names

      • CAP C antibody
      • CAPC antibody
      • Chromosome associated polypeptide C antibody
      • Chromosome-associated polypeptide C antibody
      • hCAP C antibody
      • hCAP-C antibody
      • SMC 4 antibody
      • SMC protein 4 antibody
      • SMC-4 antibody
      • SMC4 antibody
      • SMC4_HUMAN antibody
      • SMC4L1 antibody
      • Structural maintenance of chromosomes 4 antibody
      • Structural maintenance of chromosomes 4 like 1 antibody
      • Structural maintenance of chromosomes protein 4 antibody
      • XCAP C homolog antibody
      • XCAP-C homolog antibody
      see all

    Images

    • Western blot - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)
      Western blot - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)
      All lanes : Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 147 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)
      Immunocytochemistry/ Immunofluorescence - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)

      Immunofluorescent analysis of HeLa cells labeling SMC4 with ab179831 at 1/50 dilution.

    • Flow Cytometry (Intracellular) - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)
      Flow Cytometry (Intracellular) - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)

      Intracellular flow cytometric analysis of permeabilized Raji cells using ab179831 at 1/10 (red) or a rabbit IgG negative (green). 

       

       

    • Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)
      Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab179831? Please let us know so that we can cite the reference in this datasheet.

    ab179831 has been referenced in 2 publications.

    • Baergen AK  et al. Diminished Condensin Gene Expression Drives Chromosome Instability That May Contribute to Colorectal Cancer Pathogenesis. Cancers (Basel) 11:N/A (2019). PubMed: 31357676
    • Meng Q  et al. The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of the chromosome condensin complex proteins involved in replicative senescence. Biochem Biophys Res Commun 505:1112-1120 (2018). PubMed: 30336977

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