Recombinant Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)
![Western blot - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)](https://www.abcam.com/ps/products/179/ab179831/Images/ab179831-208219-anti-smc4-antibody-epr13366-c-terminal-western-blot.jpg "Western blot - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13366] to SMC4 - C-terminal
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SMC4 antibody [EPR13366] - C-terminal
See all SMC4 primary antibodies -
Description
Rabbit monoclonal [EPR13366] to SMC4 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Ramos, 293T, HeLa and Raji cell lysates. HeLa and Raji cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13366 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 147 kDa.
|
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| ICC/IF |
1/50 - 1/100.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 147 kDa. |
|
ICC/IF
1/50 - 1/100. |
Application notes
Is unsuitable for IHC-P or IP.
Target
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Function
Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. -
Tissue specificity
Widely expressed. Higher expression in testis, colon, thymus. -
Sequence similarities
Belongs to the SMC family. SMC4 subfamily. -
Domain
The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC2, forming a V-shaped heterodimer. -
Cellular localization
Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase. - Information by UniProt
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Database links
- Entrez Gene: 10051 Human
- Entrez Gene: 70099 Mouse
- Omim: 605575 Human
- SwissProt: Q9NTJ3 Human
- SwissProt: Q8CG47 Mouse
- Unigene: 58992 Human
- Unigene: 206841 Mouse
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Alternative names
- CAP C antibody
- CAPC antibody
- Chromosome associated polypeptide C antibody
see all
Images
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Western blot - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)All lanes : Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 147 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)](https://www.abcam.com/ps/products/179/ab179831/Images/ab179831-208220-anti-smc4-antibody-epr13366-c-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)Immunofluorescent analysis of HeLa cells labeling SMC4 with ab179831 at 1/50 dilution.
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![Flow Cytometry (Intracellular) - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)](https://www.abcam.com/ps/products/179/ab179831/Images/ab179831-208221-anti-smc4-antibody-epr13366-c-terminal-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)Intracellular flow cytometric analysis of permeabilized Raji cells using ab179831 at 1/10 (red) or a rabbit IgG negative (green).
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![Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)](https://www.abcam.com/ps/products/179/ab179831/Images/ab179831-4-benefits-of-recombinant-antibodies.png)
Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab179831 has been referenced in 2 publications.
- Baergen AK et al. Diminished Condensin Gene Expression Drives Chromosome Instability That May Contribute to Colorectal Cancer Pathogenesis. Cancers (Basel) 11:N/A (2019). PubMed: 31357676
- Meng Q et al. The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of the chromosome condensin complex proteins involved in replicative senescence. Biochem Biophys Res Commun 505:1112-1120 (2018). PubMed: 30336977