Recombinant Anti-Smoothelin antibody [EPR20044] (ab219652)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20044] to Smoothelin
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-Smoothelin antibody [EPR20044]
See all Smoothelin primary antibodies
Description

Rabbit monoclonal [EPR20044] to Smoothelin
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human bladder and uterus lysates; Mouse and rat colon lysates. IHC-P: Human colon, stomach, skeletal muscle, bladder cancer and gastrointestinal stromal tumor (GIST) tissues; Mouse colon and heart tissues; Rat colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20044
Isotype

IgG
Research areas

Alternative Versions
- Anti-Smoothelin antibody [EPR20044] - BSA and Azide free (ab236034)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab219652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Detects a band of approximately 59, 110 kDa (predicted molecular weight: 99 kDa).
IHC-P		1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
WB 1/1000. Detects a band of approximately 59, 110 kDa (predicted molecular weight: 99 kDa).
IHC-P 1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Function

Structural protein of the cytoskeleton.
Tissue specificity

Smooth muscle; contractile or vascular (for the long form).
Sequence similarities

Belongs to the smoothelin family.
Contains 1 CH (calponin-homology) domain.
Cellular localization

Cytoplasm > cytoskeleton. Exhibits a filamentous organization.
Target information above from: UniProt accession P53814 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6525 Human
- Entrez Gene: 29856 Mouse
- Entrez Gene: 289734 Rat
- Omim: 602127 Human
- SwissProt: P53814 Human
- SwissProt: Q921U8 Mouse
- Unigene: 149098 Human
- Unigene: 188516 Mouse
Alternative names
- FLJ35168 antibody
- FLJ35365 antibody
- FLJ35620 antibody
- FLJ38597 antibody
- Smoothelin antibody
- SMSMO antibody
- Smtn antibody
- SMTN_HUMAN antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on vascular smooth muscle and visceral smooth muscle of human colon is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on visceral smooth muscle of human stomach is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on vascular smooth muscle of human bladder cancer. Negative staining on tumor cells. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor (GIST) tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on scattered tumor cells of human gastrointestinal stromal tumor (PMID: 19950405). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on vascular smooth muscle of skeletal muscle. Negative staining on skeletal muscle cells. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on vascular smooth muscle and visceral smooth muscle of mouse colon is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

Immunohistochemical analysis of paraffin-embedded mouse heart tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse cardiac vascular smooth muscle cells. Negative staining on myocardium. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on vascular smooth muscle and visceral smooth muscle of rat colon is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [EPR20044] (ab219652)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Smoothelin with ab219652 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Negative control: No staining on rat skeletal muscle (PMID: 8707825).

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Smoothelin antibody [EPR20044] (ab219652)

All lanes : Anti-Smoothelin antibody [EPR20044] (ab219652) at 1/1000 dilution

Lane 1 : Human bladder lysate at 20 µg
Lane 2 : Human uterus lysate at 10 µg

Secondary
Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/4000 dilution
Lane 2 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 99 kDa
Observed band size: 110,59 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 3 minutes; Lane 2: 3 seconds.

This antibody reacts with the 59 kDa and 110 kDa bands, corresponding to isoforms A and B respectively. Smoothelin is exclusively found in smooth muscle cells (PMID:8707825).
Western blot - Anti-Smoothelin antibody [EPR20044] (ab219652)

All lanes : Anti-Smoothelin antibody [EPR20044] (ab219652) at 1/1000 dilution

Lane 1 : Mouse colon lysate
Lane 2 : Rat colon lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 99 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 30 seconds; Lane 2: 2 seconds.

This antibody reacts with a 59 kDa band in mouse and rat colon smooth muscle.
Anti-Smoothelin antibody [EPR20044] (ab219652)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab219652? Please let us know so that we can cite the reference in this datasheet.

ab219652 has been referenced in 1 publication.

Lourenssen SR & Blennerhassett MG M2 macrophages and phenotypic modulation of intestinal smooth muscle cells characterize Inflammatory stricture formation in the rat. Am J Pathol N/A:N/A (2020). PubMed: 32479820

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (1)

Customer reviews and Q&As