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  1. Link

    products/primary-antibodies/smoothelin-antibody-r4a-ab8969.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class III Smoothelins
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Anti-Smoothelin antibody [R4A] (ab8969)

  • Datasheet
  • SDS
Reviews (5)Q&A (2)References (27)

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Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)
  • Western blot - Anti-Smoothelin antibody [R4A] (ab8969)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [R4A] (ab8969)
  • Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)
  • Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)
  • Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)
  • Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)
  • Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)
  • Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)
  • Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

Key features and details

  • Mouse monoclonal [R4A] to Smoothelin
  • Suitable for: ICC/IF, IHC-Fr, IHC-P, WB
  • Reacts with: Chicken, Cat, Dog, Human, Pig, Monkey
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Smoothelin antibody [R4A]
    See all Smoothelin primary antibodies
  • Description

    Mouse monoclonal [R4A] to Smoothelin
  • Host species

    Mouse
  • Specificity

    Reacts with the 59 kDa and 100 kDa protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells.

  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Chicken, Cat, Dog, Human, Pig, Monkey
    Does not react with: Mouse, Rat, Hamster
  • Immunogen

    Recombinant full length protein corresponding to Smoothelin. Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal extract of chicken gizzard.
    Database link: P53814

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    R4A
  • Myeloma

    Sp2/0-Ag14
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class III
    • Smoothelins
    • Cardiovascular
    • Vasculature
    • Smooth muscle cell (SMC)
    • Cytoskeleton
    • Developmental Biology
    • Organogenesis
    • Skeletal development
    • Muscle
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Smooth Muscle Cells

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P (3)
Use at an assay dependent concentration.
WB (1)
1/100 - 1/1000.
Notes
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
1/100 - 1/1000.

Target

  • Function

    Structural protein of the cytoskeleton.
  • Tissue specificity

    Smooth muscle; contractile or vascular (for the long form).
  • Sequence similarities

    Belongs to the smoothelin family.
    Contains 1 CH (calponin-homology) domain.
  • Cellular localization

    Cytoplasm > cytoskeleton. Exhibits a filamentous organization.
  • Target information above from: UniProt accession P53814 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 373982 Chicken
    • Entrez Gene: 6525 Human
    • Omim: 602127 Human
    • SwissProt: P53814 Human
    • Unigene: 149098 Human
    • Alternative names

      • FLJ35168 antibody
      • FLJ35365 antibody
      • FLJ35620 antibody
      • FLJ38597 antibody
      • Smoothelin antibody
      • SMSMO antibody
      • Smtn antibody
      • SMTN_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)
      Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

      Smooth muscle cells in swine colon as detected by ab8969 (dilution 1:100).

    • Western blot - Anti-Smoothelin antibody [R4A] (ab8969)
      Western blot - Anti-Smoothelin antibody [R4A] (ab8969)
      All lanes : Anti-Smoothelin antibody [R4A] (ab8969)

      Lane 1 : uterine artery
      Lane 2 : uterine corpus.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [R4A] (ab8969)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [R4A] (ab8969)

      Immunohistochemical analysis on paraffin section of human smooth muscle labeling Smoothelin with ab8969.

    • Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)
      Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)

      Indirect immunofluorescence staining of a cultured human fibroblast, showing colocalization of smoothelin (red fluorescence; as detected by ab8969) and actin (green fluorescence).

    • Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)
      Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

      Smooth muscle cells in swine colon as detected by ab8969 (dilution 1:100).

    • Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)
      Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

      Smooth muscle cells in swine colon as detected by ab8969 (dilution 1:100).

    • Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)
      Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)

      Human blood vessel in cardiac muscle stained with the monoclonal smoothelin antibody ab8969.

    • Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)
      Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

      Immunohistochemical analysis on frozen section of chicken gizzard striated muscle labeling Smoothelin with ab8969.

    • Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)
      Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)This image is courtesy of an anonymous Abreview
      ab8969 staining cells from a human vascular smooth muscle cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 10 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 21°C. An Rhodamine conjugated donkey anti-mouse antibody, diluted 1/200, was used as the secondary.

      See Abreview

    • Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)
      Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

      Immunohistochemistry on frozen section of chicken gizzard striated muscle labeling Smoothelin with ab8969.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (27)

    Publishing research using ab8969? Please let us know so that we can cite the reference in this datasheet.

    ab8969 has been referenced in 27 publications.

    • Ding H  et al. Differentially expressed mRNAs and their upstream miR-491-5p in patients with coronary atherosclerosis as well as the function of miR-491-5p in vascular smooth muscle cells. Korean J Physiol Pharmacol 26:183-193 (2022). PubMed: 35477546
    • Xia Y  et al. MIRLET7BHG promotes hepatocellular carcinoma progression by activating hepatic stellate cells through exosomal SMO to trigger Hedgehog pathway. Cell Death Dis 12:326 (2021). PubMed: 33771969
    • Tao W  et al. MicroRNA-199a-5p aggravates angiotensin II-induced vascular smooth muscle cell senescence by targeting Sirtuin-1 in abdominal aortic aneurysm. J Cell Mol Med 25:6056-69 (2021). PubMed: 34132029
    • Jin YP  et al. Encapsulated three-dimensional bioprinted structure seeded with urothelial cells: a new construction technique for tissue-engineered urinary tract patch. Chin Med J (Engl) 133:424-434 (2020). PubMed: 31977553
    • Ackers I  et al. Oxidized Low-Density Lipoprotein Induces WNT5A Signaling Activation in THP-1 Derived Macrophages and a Human Aortic Vascular Smooth Muscle Cell Line. Front Cardiovasc Med 7:567837 (2020). PubMed: 33330641
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Smoothelin antibody [R4A]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Sheep Tissue sections (Carotid Artery)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Carotid Artery
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Le Zhen

    Verified customer

    Submitted Aug 23 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Smoothelin antibody [R4A]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (Carotid Artery)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM Citrate Buffer (pH=6)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    Carotid Artery
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 20 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Smoothelin antibody [R4A]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue lysate - other (canine myocardial infarct)
    Specification
    canine myocardial infarct
    Antigen retrieval step
    None
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Marcin Dobaczewski

    Verified customer

    Submitted Aug 11 2005

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