Name: Anti-Smoothelin antibody [R4A]
SKU: ab8969
Rating: 5 (5 reviews)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

Loading size & price…

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

Key features and details

Mouse monoclonal [R4A] to Smoothelin
Suitable for: ICC/IF, IHC-Fr, IHC-P, WB
Reacts with: Chicken, Cat, Dog, Human, Pig, Monkey
Isotype: IgG1

Product name

Anti-Smoothelin antibody [R4A]
See all Smoothelin primary antibodies
Description

Mouse monoclonal [R4A] to Smoothelin
Host species

Mouse
Specificity

Reacts with the 59 kDa and 100 kDa protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells.
Tested applications

Suitable for: ICC/IF, IHC-Fr, IHC-P, WBmore details
Species reactivity

Reacts with: Chicken, Cat, Dog, Human, Pig, Monkey
Does not react with: Mouse, Rat, Hamster
Immunogen

Recombinant full length protein corresponding to Smoothelin. Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal extract of chicken gizzard.
Database link: P53814
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

R4A
Myeloma

Sp2/0-Ag14
Isotype

IgG1
Research areas

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF	(1)	Use at an assay dependent concentration.
IHC-Fr		Use at an assay dependent concentration.
IHC-P	(3)	Use at an assay dependent concentration.
WB	(1)	1/100 - 1/1000.

Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/100 - 1/1000.

Function

Structural protein of the cytoskeleton.
Tissue specificity

Smooth muscle; contractile or vascular (for the long form).
Sequence similarities

Belongs to the smoothelin family.
Contains 1 CH (calponin-homology) domain.
Cellular localization

Cytoplasm > cytoskeleton. Exhibits a filamentous organization.
Target information above from: UniProt accession P53814 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 373982 Chicken
- Entrez Gene: 6525 Human
- Omim: 602127 Human
- SwissProt: P53814 Human
- Unigene: 149098 Human
Alternative names
- FLJ35168 antibody
- FLJ35365 antibody
- FLJ35620 antibody
- FLJ38597 antibody
- Smoothelin antibody
- SMSMO antibody
- Smtn antibody
- SMTN_HUMAN antibody
see all

Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

Smooth muscle cells in swine colon as detected by ab8969 (dilution 1:100).
Western blot - Anti-Smoothelin antibody [R4A] (ab8969)

All lanes : Anti-Smoothelin antibody [R4A] (ab8969)

Lane 1 : uterine artery
Lane 2 : uterine corpus.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [R4A] (ab8969)

Immunohistochemical analysis on paraffin section of human smooth muscle labeling Smoothelin with ab8969.
Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)

Indirect immunofluorescence staining of a cultured human fibroblast, showing colocalization of smoothelin (red fluorescence; as detected by ab8969) and actin (green fluorescence).
Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

Smooth muscle cells in swine colon as detected by ab8969 (dilution 1:100).
Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

Smooth muscle cells in swine colon as detected by ab8969 (dilution 1:100).
Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)

Human blood vessel in cardiac muscle stained with the monoclonal smoothelin antibody ab8969.
Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

Immunohistochemical analysis on frozen section of chicken gizzard striated muscle labeling Smoothelin with ab8969.
Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)This image is courtesy of an anonymous Abreview

ab8969 staining cells from a human vascular smooth muscle cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 10 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 21°C. An Rhodamine conjugated donkey anti-mouse antibody, diluted 1/200, was used as the secondary.
See Abreview
Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

Immunohistochemistry on frozen section of chicken gizzard striated muscle labeling Smoothelin with ab8969.

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab8969? Please let us know so that we can cite the reference in this datasheet.

ab8969 has been referenced in 27 publications.

Ding H et al. Differentially expressed mRNAs and their upstream miR-491-5p in patients with coronary atherosclerosis as well as the function of miR-491-5p in vascular smooth muscle cells. Korean J Physiol Pharmacol 26:183-193 (2022). PubMed: 35477546
Xia Y et al. MIRLET7BHG promotes hepatocellular carcinoma progression by activating hepatic stellate cells through exosomal SMO to trigger Hedgehog pathway. Cell Death Dis 12:326 (2021). PubMed: 33771969
Tao W et al. MicroRNA-199a-5p aggravates angiotensin II-induced vascular smooth muscle cell senescence by targeting Sirtuin-1 in abdominal aortic aneurysm. J Cell Mol Med 25:6056-69 (2021). PubMed: 34132029
Jin YP et al. Encapsulated three-dimensional bioprinted structure seeded with urothelial cells: a new construction technique for tissue-engineered urinary tract patch. Chin Med J (Engl) 133:424-434 (2020). PubMed: 31977553
Ackers I et al. Oxidized Low-Density Lipoprotein Induces WNT5A Signaling Activation in THP-1 Derived Macrophages and a Human Aortic Vascular Smooth Muscle Cell Line. Front Cardiovasc Med 7:567837 (2020). PubMed: 33330641

View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Sheep Tissue sections (Carotid Artery)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9.0

Permeabilization

Yes - 0.05% Tween 20

Specification

Carotid Artery

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 22°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Le Zhen

Verified customer

Submitted Aug 23 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rabbit Tissue sections (Carotid Artery)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10 mM Citrate Buffer (pH=6)

Permeabilization

Yes - 0.1% Triton X-100

Specification

Carotid Artery

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 20 2016

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Dog Tissue lysate - other (canine myocardial infarct)

Specification

canine myocardial infarct

Antigen retrieval step

None

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 2%

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Marcin Dobaczewski

Verified customer

Submitted Aug 11 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Myeloma

Isotype

Research areas

Associated products

Compatible Secondaries

Isotype control

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (27)

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Smoothelin antibody [R4A]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Smoothelin antibody [R4A]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Smoothelin antibody [R4A]