Anti-SNAIL + SLUG antibody (ab180714)
Key features and details
- Rabbit polyclonal to SNAIL + SLUG
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SNAIL + SLUG antibody
See all SNAIL + SLUG primary antibodies -
Description
Rabbit polyclonal to SNAIL + SLUG -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Synthetic peptide within Human SNAIL+SLUG aa 236-264. The exact sequence is proprietary.
Database link: O95863 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 29 kDa.
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| IHC-P | (1) |
1/50 - 1/200.
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| Notes |
|---|
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WB
1/500 - 1/2000. Predicted molecular weight: 29 kDa. |
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IHC-P
1/50 - 1/200. |
Target
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Relevance
Function: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. -
Cellular localization
Slug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs. -
Database links
- Entrez Gene: 6591 Human
- Entrez Gene: 6615 Human
- Entrez Gene: 20583 Mouse
- Entrez Gene: 20613 Mouse
- Entrez Gene: 116490 Rat
- Entrez Gene: 25554 Rat
- Omim: 604238 Human
- SwissProt: O43623 Human
see all -
Alternative names
- dJ710H13.1 antibody
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
see all
Images
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Western blot - Anti-SNAIL + SLUG antibody (ab180714)Anti-SNAIL + SLUG antibody (ab180714) at 1/1000 dilution + Mouse kidney at 25 µg
Secondary
HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 29 kDa
Exposure time: 180 secondsBlocking buffer: 3% nonfat dry milk in TBST.
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Western blot - Anti-SNAIL + SLUG antibody (ab180714)All lanes : Anti-SNAIL + SLUG antibody (ab180714) at 1/1000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 29 kDa
Exposure time: 90 secondsBlocking buffer: 3% nonfat dry milk in TBST.
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Western blot - Anti-SNAIL + SLUG antibody (ab180714)Anti-SNAIL + SLUG antibody (ab180714) at 1/500 dilution + Mouse heart tissue extracts
Predicted band size: 29 kDa -
Western blot - Anti-SNAIL + SLUG antibody (ab180714)All lanes : Anti-SNAIL + SLUG antibody (ab180714)
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Mouse lung tissue lysate
Lane 5 : Rat liver tissue lysate
Predicted band size: 29 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (ab180714)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SNAI1+SLUG with ab180714 at 1/50 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (ab180714)Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling SNAIL + SLUG with ab180714 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (ab180714)Image is courtesy of an anonymous Abreviewab180714 staining SNAIL in Mouse kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation in a basic buffer (TRIS+EDTA, pH9). Samples were incubated with primary antibody (1/500 in antibody diluent) for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (ab180714)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification: 200X.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (ab180714)Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling SNAIL +SLUG with ab180714 at 1/100 dilution.
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Western blot - Anti-SNAIL + SLUG antibody (ab180714)All lanes : Anti-SNAIL + SLUG antibody (ab180714)
Lane 1 : Snail recombinant with GST tag (55 kDa)
Lane 2 : Slug recombinant with GST tag (56 kDa)
Lysates/proteins at 0.1 µg per lane.
Predicted band size: 29 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAIL + SLUG antibody (ab180714)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAIL with ab180714. Tissue was fixed with formaldehyde and blocked with 5% BSA for 20 min at room temperature; antigen retrieval was by heat mediation with EDTA buffer (pH 9.0). Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated mouse anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Magnification: 400X.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (185)
ab180714 has been referenced in 185 publications.
- Li J et al. METTL3 promotes prostatic hyperplasia by regulating PTEN expression in an m6A-YTHDF2-dependent manner. Cell Death Dis 13:723 (2022). WB ; Human . PubMed: 35985997
- Bukovac A et al. Bilateral Meningioma: A Case Report and Review of the Literature. Int J Mol Sci 23:N/A (2022). PubMed: 35163107
- Shan Q et al. Significance of cancer stroma for bone destruction in oral squamous cell carcinoma using different cancer stroma subtypes. Oncol Rep 47:N/A (2022). PubMed: 35211756
- Park Y et al. Impact of Exogenous Treatment with Histidine on Hepatocellular Carcinoma Cells. Cancers (Basel) 14:N/A (2022). PubMed: 35267513
- Wang Z et al. 3BDO inhibits the proliferation, epithelial-mesenchymal transition (EMT), and stemness via suppressing survivin in human glioblastoma cells. J Cancer 13:1203-1213 (2022). PubMed: 35281871