Anti-SNM1A antibody (ab226003)
Key features and details
- Rabbit polyclonal to SNM1A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SNM1A antibody
See all SNM1A primary antibodies -
Description
Rabbit polyclonal to SNM1A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Gorilla -
Immunogen
Synthetic peptide within Human SNM1A aa 425-475. The exact sequence is proprietary. (NP_055696.3).
Database link: Q6PJP8 -
Positive control
- WB: HEK-293T, HeLa and Jurkat whole cell lysate (ab7899). IP: HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab226003 was affinity purified using an epitope specific to SNM1A immobilized on solid support -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 116 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 116 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
May be required for DNA interstrand cross-link repair. Also required for checkpoint mediated cell cycle arrest in early prophase in response to mitotic spindle poisons. -
Tissue specificity
Expressed in brain, heart, kidney, liver, pancreas, placenta and skeletal muscle. -
Sequence similarities
Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family. -
Cellular localization
Nucleus. In some cells it may be found in typically 1 or 2 discrete nuclear aggregates of unknown function which also contain TP53BP1. Also found in multiple discrete nuclear foci which increase in number following treatment with ionizing radiation or interstrand cross-linking agents. These foci overlap with those formed by the MRN complex (composed of MRE11A, RAD50 and NBN) and BRCA1. - Information by UniProt
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Database links
- Entrez Gene: 101145990 Gorilla
- Entrez Gene: 9937 Human
- Omim: 609682 Human
- SwissProt: Q6PJP8 Human
- Unigene: 1560 Human
- Unigene: 703616 Human
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Alternative names
- Dclre1a antibody
- DCR1A_HUMAN antibody
- DNA cross link repair 1A antibody
see all
Images
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All lanes : Anti-SNM1A antibody (ab226003) at 0.1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 116 kDa
Exposure time: 3 minutes -
SNM1A was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226003 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226003 at 1 µg/ml.
Lane 1: ab226003 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226003 has not yet been referenced specifically in any publications.