Recombinant Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
![Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)](https://www.abcam.com/ps/products/68/ab68155/Images/ab68155-5-anti-sod2-antibody-epr2560y-western-blot-human-lysate.jpg "Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2560Y] to SOD2/MnSOD
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SOD2/MnSOD antibody [EPR2560Y]
See all SOD2/MnSOD primary antibodies -
Description
Rabbit monoclonal [EPR2560Y] to SOD2/MnSOD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human SOD2/MnSOD aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- WB: SH-SY5Y, A549, HEK-293T and HAP1 cell lysates; Human heart tissue; Rat brain and heart lysate. IHC-P: Human liver tissue sections. IHC-Fr: Rat lung tissue sections.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2560Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab68155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
1/1000 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. -
Involvement in disease
Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
Sequence similarities
Belongs to the iron/manganese superoxide dismutase family. -
Post-translational
modificationsNitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 6648 Human
- Entrez Gene: 20656 Mouse
- Entrez Gene: 24787 Rat
- Omim: 147460 Human
- SwissProt: P04179 Human
- SwissProt: P09671 Mouse
- SwissProt: P07895 Rat
- Unigene: 487046 Human
see all -
Alternative names
- Indophenoloxidase B antibody
- IPO B antibody
- IPOB antibody
see all
Images
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Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/1000 dilution
Lane 1 : SHSY5Y cell lysate
Lane 2 : A549 cell lysate
Lane 3 : Wild-type HEK-293T cell lysate
Lane 4 : SOD2 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 25 kDaLanes 1 - 4: Merged signal (red and green). Green - ab68155 observed at 22 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab68155 was shown to react with SOD2/MnSOD in wild-type HEK-293T. Loss of signal was observed when knockout cell line ab255434 (knockout cell lysate ab263835) was used. Wild-type and SOD2/MnSOD knockout samples were subjected to SDS-PAGE. ab68155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)](https://www.abcam.com/ps/products/68/ab68155/Images/ab68155-261350-ab68155SOD2.jpg)
Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : SOD2 knockout HAP1 cell lysate
Lane 3 : Human heart tissue lysate
Lane 4 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 25 kDaLanes 1 - 4: Merged signal (red and green). Green - ab68155 observed at 29 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab68155 was shown to specifically react with SOD2 when SOD2 knockout samples were used. Wild-type and SOD2 knockout samples were subjected to SDS-PAGE. ab68155 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)](https://www.abcam.com/ps/products/68/ab68155/Images/ab68155_1.jpg)
Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/500 dilution
Lane 1 : Rat brain lysate
Lane 2 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)](https://www.abcam.com/ps/products/68/ab68155/Images/ab68155_3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)ab68155 at 1:100 dilution staining Superoxide Dismutase 2 in human liver tissue sections.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)](https://www.abcam.com/ps/products/68/ab68155/Images/ab68155-6-benefits-of-recombinant-antibodies.png)
Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (36)
ab68155 has been referenced in 36 publications.
- Kitauchi S et al. Effects of in utero and lactational exposure to the no-observed-adverse-effect level (NOAEL) dose of the neonicotinoid clothianidin on the reproductive organs of female mice. J Vet Med Sci 83:746-753 (2021). PubMed: 33563863
- Jayakumari NR et al. Honokiol regulates mitochondrial substrate utilization and cellular fatty acid metabolism in diabetic mice heart. Eur J Pharmacol 896:173918 (2021). PubMed: 33529726
- Wan S et al. Dot1l Aggravates Keratitis Induced by Herpes Simplex Virus Type 1 in Mice via p38 MAPK-Mediated Oxidative Stress. Oxid Med Cell Longev 2021:6612689 (2021). PubMed: 33628364
- Chang WT et al. A Marine Terpenoid, Heteronemin, Induces Both the Apoptosis and Ferroptosis of Hepatocellular Carcinoma Cells and Involves the ROS and MAPK Pathways. Oxid Med Cell Longev 2021:7689045 (2021). PubMed: 33488943
- James J et al. Single Mutation in the NFU1 Gene Metabolically Reprograms Pulmonary Artery Smooth Muscle Cells. Arterioscler Thromb Vasc Biol 41:734-754 (2021). PubMed: 33297749