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    products/primary-antibodies/sod2mnsod-antibody-epr2560y-ab68155.pdf

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Cell Biology Apoptosis Mitochondrial
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (36)

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Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
  • Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
  • Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
  • Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2560Y] to SOD2/MnSOD
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Knockout
Product image
Human SOD2 (MnSOD) knockout HEK-293T cell line (ab255434)
Knockout
Product image
Human SOD2 (MnSOD) knockout HEK-293T cell lysate (ab263835)
Assay
Product image
Superoxide Dismutase Activity Assay Kit (Colorimetric) (ab65354)

View more associated products

Overview

  • Product name

    Anti-SOD2/MnSOD antibody [EPR2560Y]
    See all SOD2/MnSOD primary antibodies
  • Description

    Rabbit monoclonal [EPR2560Y] to SOD2/MnSOD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SOD2/MnSOD aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: SH-SY5Y, A549, HEK-293T and HAP1 cell lysates; Human heart tissue; Rat brain and heart lysate. IHC-P: Human liver tissue sections. IHC-Fr: Rat lung tissue sections.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2560Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-SOD2/MnSOD antibody [EPR2560Y] - BSA and Azide free (ab227846)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human SOD2 (MnSOD) knockout HEK-293T cell line (ab255434)
  • KO cell lysates

    • Human SOD2 (MnSOD) knockout HEK-293T cell lysate (ab263835)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab68155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
  • Involvement in disease

    Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • Sequence similarities

    Belongs to the iron/manganese superoxide dismutase family.
  • Post-translational
    modifications

    Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession P04179 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6648 Human
    • Entrez Gene: 20656 Mouse
    • Entrez Gene: 24787 Rat
    • Omim: 147460 Human
    • SwissProt: P04179 Human
    • SwissProt: P09671 Mouse
    • SwissProt: P07895 Rat
    • Unigene: 487046 Human
    • Unigene: 290876 Mouse
    • Unigene: 10488 Rat
    see all
  • Alternative names

    • Indophenoloxidase B antibody
    • IPO B antibody
    • IPOB antibody
    • Manganese containing superoxide dismutase antibody
    • Manganese SOD antibody
    • Manganese superoxide dismutase antibody
    • Mangano superoxide dismutase antibody
    • Mn SOD antibody
    • Mn superoxide dismutase antibody
    • MNSOD antibody
    • MVCD6 antibody
    • SOD 2 antibody
    • SOD2 antibody
    • SODM_HUMAN antibody
    • Superoxide dismutase [Mn] mitochondrial antibody
    • Superoxide dismutase [Mn], mitochondrial antibody
    • Superoxide dismutase 2 mitochondrial antibody
    see all

Images

  • Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
    Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
    All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/1000 dilution

    Lane 1 : SHSY5Y cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : Wild-type HEK-293T cell lysate
    Lane 4 : SOD2 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 25 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab68155 observed at 22 kDa. Red - loading control, ab8245 observed at 37 kDa.  

     ab68155 was shown to react with SOD2/MnSOD in wild-type HEK-293T. Loss of signal was observed when knockout cell line ab255434 (knockout cell lysate ab263835) was used. Wild-type and SOD2/MnSOD knockout samples were subjected to SDS-PAGE. ab68155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
    Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
    All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : SOD2 knockout HAP1 cell lysate
    Lane 3 : Human heart tissue lysate
    Lane 4 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 25 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab68155 observed at 29 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab68155 was shown to specifically react with SOD2 when SOD2 knockout samples were used. Wild-type and SOD2 knockout samples were subjected to SDS-PAGE. ab68155 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
    Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
    All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/500 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : SH-SY5Y lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)

    ab68155 at 1:100 dilution staining Superoxide Dismutase 2 in human liver tissue sections.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)
    Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155)

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (36)

Publishing research using ab68155? Please let us know so that we can cite the reference in this datasheet.

ab68155 has been referenced in 36 publications.

  • Kitauchi S  et al. Effects of in utero and lactational exposure to the no-observed-adverse-effect level (NOAEL) dose of the neonicotinoid clothianidin on the reproductive organs of female mice. J Vet Med Sci 83:746-753 (2021). PubMed: 33563863
  • Jayakumari NR  et al. Honokiol regulates mitochondrial substrate utilization and cellular fatty acid metabolism in diabetic mice heart. Eur J Pharmacol 896:173918 (2021). PubMed: 33529726
  • Wan S  et al. Dot1l Aggravates Keratitis Induced by Herpes Simplex Virus Type 1 in Mice via p38 MAPK-Mediated Oxidative Stress. Oxid Med Cell Longev 2021:6612689 (2021). PubMed: 33628364
  • Chang WT  et al. A Marine Terpenoid, Heteronemin, Induces Both the Apoptosis and Ferroptosis of Hepatocellular Carcinoma Cells and Involves the ROS and MAPK Pathways. Oxid Med Cell Longev 2021:7689045 (2021). PubMed: 33488943
  • James J  et al. Single Mutation in the NFU1 Gene Metabolically Reprograms Pulmonary Artery Smooth Muscle Cells. Arterioscler Thromb Vasc Biol 41:734-754 (2021). PubMed: 33297749
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOD2/MnSOD antibody [EPR2560Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 13 2019

Western blot abreview for Anti-SOD2 antibody [EPR2560Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12% tris-glycine gel)
Sample
Human Tissue lysate - whole (heart)
Specification
heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 23 2014

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