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    products/primary-antibodies/somatostatin-28-antibody-epr33592-ab111912.pdf

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RecombinantRabMAb

Recombinant Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (2)

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Multiplex immunohistochemistry - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
  • Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
  • Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
  • Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
  • Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
  • Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3359(2)] to Somatostatin 28
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Somatostatin 28 antibody [EPR3359(2)]
    See all Somatostatin 28 primary antibodies
  • Description

    Rabbit monoclonal [EPR3359(2)] to Somatostatin 28
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
    Unsuitable for: Flow Cyt,ICC/IF,IP or WB
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human,rat and mouse pancreas tissue; IHC-Fr: Rat hippocampal and pancrease tissue, Mouse hippocampal and pancreas tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3359(2)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Neuropeptides
    • Hormones
    • Neuroscience
    • Neurology process
    • Neuroendocrinology
    • GH Regulation
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Developmental Biology
    • Lineage specification
    • Endoderm

Associated products

  • Alternative Versions

    • Anti-Somatostatin 28 antibody [EPR3359(2)] - BSA and Azide free (ab227422)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab111912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (4)
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
Notes
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IP or WB.

Target

  • Relevance

    The cyclic tetradecapeptide somatostatin 28 is widely distributed throughout the body and is an important regulator of endocrine and nervous system function. It exerts its biologic actions by binding to specific high affinity receptors on the cell surface.
  • Cellular localization

    Secreted
  • Database links

    • Entrez Gene: 6750 Human
    • Entrez Gene: 20604 Mouse
    • Entrez Gene: 24797 Rat
    • Omim: 182450 Human
    • SwissProt: P61278 Human
    • SwissProt: P60041 Mouse
    • SwissProt: P60042 Rat
    • Alternative names

      • Growth hormone release inhibiting factor antibody
      • SMST antibody
      • Somatostatin 28 antibody
      • Somatostatin antibody
      • SST antibody
      see all

    Images

    • Multiplex immunohistochemistry - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
      Multiplex immunohistochemistry - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

      Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat pancreas tissue.

      Panel A: Merged staining of anti-Pancreatic Polypeptide (ab255827, green), anti-Somatostatin 28 (ab111912, red) and DAPI (blue).

      Panel B: Anti-Pancreatic Polypeptide (green) stained on PP cells in rat pancreas islet.

      Panel C: Anti-Somatostatin 28 (red) stained on delta cells in rat pancreas islet.

      Panel D: Nuclear counter stain, DAPI (blue).

      Key protocol steps:  The section was incubated in two rounds of staining with ab255827 (1/20000 dilution) and ab111912 (1/2000 dilution) for 30 mins at room temperature. Each round was followed by tyramide signal amplification with the appropriate fluorophore. Heat mediated antigen retrieval was used (Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins after every round of antibody/fluorophore staining.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      A ready-to-use anti-Rabbit and Mouse Polymer HRP was used as a secondary.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

      ab111912, at a 1/50 dilution, staining Somatostatin 28 in paraffin embedded Human pancreas tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
      Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

      Ab111912 staining Somatostatin-28 in Rat hippocampus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).  Primary antibody was used at a 1:500 dilution. Ab150077 (1:1000) was used as the secondary antibody. Counterstained with DAPI. Cytoplasmic staining in the hilus of the dentate gyrus of rat hippocampus [PMID: 25469233] . 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

      ab111912 staining Somatostatin 28 in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat mediated antigen retrieval in a TRIS/EDTA buffer pH9 (ab93684). Primary antibody at 1:4000 dilution and   a  Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Couterstained with hematoxylin. Cytoplasmic staining on delta cells of mouse pancreas islet [PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32] . 

       

       

    • Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
      Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

      Ab111912 staining Somatostatin-28 in Rat pancreas tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).Primary antibody was used at a 1:500 dilution. Ab150077 (1:1000) was used as the secondary antibody.Counterstained with DAPI. Cytoplasmic staining on delta cells of rat pancreas islet. [PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32] .  

    • Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
      Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

      Ab111912 staining Somatostatin-28 in Mouse hippocampus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100.Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Primary antibody was used at a 1:500 dilution. Ab150077 (1:1000) was used as the secondary antibody.Counterstained with DAPI.  Cytoplasmic staining in the hilus of the dentate gyrus of mouse hippocampus [PMID: 25469233]. 

    • Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
      Immunohistochemistry (Frozen sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

      Ab111912 staining Somatostatin-28 in Mouse pancreas tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% TritonX-100. Heat mediated antigen retrieval was performed using a citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Primary antibody was used at a 1:500 dilution. Ab150077 (1:1000) was used as the secondary antibody. Counterstained with DAPI. Cytoplasmic staining on delta cells of mouse pancreas islet. [PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32] . 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

      ab111912 staining Somatostatin 28 in Rat pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediated in a TRIS/EDTA buffer pH9 (ab93684).Primary antibody at 1:4000 dilution and Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin. Cytoplasmic staining on delta cells of rat pancreas islet[PMID: 25019573], [PMID: 9066918], [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32]  .

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

      ab111912 staining Somatostatin 28 in human pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Heat mediated antigen retrieval in a TRIS/EDTA buffer pH9 (ab93684). Primary antibody  at 1/4000 dilution and a Goat Anti- rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin. Cytoplasmic staining on delta cells of human pancreas islet. [PMID: 25019573], [PMID: 9066918],  [BiologiaSerbica 2014, Vol. 36  No. 1-2 24-32] . 

    • Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)
      Anti-Somatostatin 28 antibody [EPR3359(2)] (ab111912)

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab111912? Please let us know so that we can cite the reference in this datasheet.

    ab111912 has been referenced in 2 publications.

    • Wiebe S  et al. The eIF4E homolog 4EHP (eIF4E2) regulates hippocampal long-term depression and impacts social behavior. Mol Autism 11:92 (2020). PubMed: 33225984
    • Simonet JC  et al. Conditional Loss of Arx From the Developing Dorsal Telencephalon Results in Behavioral Phenotypes Resembling Mild Human ARX Mutations. Cereb Cortex N/A:N/A (2014). Mouse . PubMed: 24794919

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Somatostatin 28 antibody [EPR3359(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted May 19 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Somatostatin 28 antibody [EPR3359(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Spinal cord
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted May 17 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Somatostatin 28 antibody [EPR3359(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.4
    Permeabilization
    No
    Specification
    Spinal cord
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Yazead Buhidma

    Verified customer

    Submitted May 08 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Somatostatin 28 antibody [EPR3359(2)]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Pancreas)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Pancreas
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    Submitted Apr 18 2021

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