Anti-Somatostatin antibody [7G5] (ab140665)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin antibody [7G5] (ab140665)](https://www.abcam.com/ps/products/140/ab140665/Images/Somatostatin-Primary-antibodies-ab140665-4.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin antibody [7G5] (ab140665)")
Key features and details
- Mouse monoclonal [7G5] to Somatostatin
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Somatostatin antibody [7G5]
See all Somatostatin primary antibodies -
Description
Mouse monoclonal [7G5] to Somatostatin -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to Human Somatostatin aa 1 to the C-terminus. Expressed in E. coli
Database link: P61278 -
Positive control
- Recombinant Human Somatostatin protein; Human HepG2 cell lysate; Human lung cancer and pancreas tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
7G5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 13 kDa.
|
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| IHC-P | (2) |
1/200 - 1/1000.
|
| Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 13 kDa. |
|
IHC-P
1/200 - 1/1000. |
|
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Somatostatin inhibits the release of somatotropin. -
Sequence similarities
Belongs to the somatostatin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6750 Human
- Omim: 182450 Human
- SwissProt: P61278 Human
- Unigene: 12409 Human
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Alternative names
- Growth hormone release inhibiting factor antibody
- Growth hormone release-inhibiting factor antibody
- Prepro somatostatin antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin antibody [7G5] (ab140665)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Somatostatin with ab140665 at a 1/200 dilution followed by DAB staining.
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![Flow Cytometry - Anti-Somatostatin antibody [7G5] (ab140665)](https://www.abcam.com/ps/products/140/ab140665/Images/Somatostatin-Primary-antibodies-ab140665-2.jpg)
Flow Cytometry - Anti-Somatostatin antibody [7G5] (ab140665)Flow cytometric analysis of HepG2 cells labelling Somatostatin with ab140665 at 1/200 dilution (green); negative control (red). -
![Western blot - Anti-Somatostatin antibody [7G5] (ab140665)](https://www.abcam.com/ps/products/140/ab140665/Images/Somatostatin-Primary-antibodies-ab140665-3.jpg)
Western blot - Anti-Somatostatin antibody [7G5] (ab140665)Anti-Somatostatin antibody [7G5] (ab140665) at 1/500 dilution + Somatostatin recombinant protein
Predicted band size: 13 kDa
Expected MW is 38.2 kDa. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin antibody [7G5] (ab140665)](https://www.abcam.com/ps/products/140/ab140665/Images/Somatostatin-Primary-antibodies-ab140665-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin antibody [7G5] (ab140665)Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Somatostatin with ab140665 at a 1/200 dilution followed by DAB staining.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab140665 has been referenced in 2 publications.
- Al-Amily IM et al. The functional impact of G protein-coupled receptor 142 (Gpr142) on pancreatic ß-cell in rodent. Pflugers Arch 471:633-645 (2019). PubMed: 30767071
- Wang Y et al. An optical method to evaluate both mass and functional competence of pancreatic a- and ß-cells. J Cell Sci 129:2462-71 (2016). PubMed: 27173492
