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RecombinantRabMAb

Recombinant Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)

Submit a review Q&A (3)References (89)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
  • Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
  • Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
  • Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
  • Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [UMB1] to Somatostatin Receptor 2 - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal
    See all Somatostatin Receptor 2 primary antibodies

  • Description

    Rabbit monoclonal [UMB1] to Somatostatin Receptor 2 - C-terminal

  • Host species

    Rabbit

  • Specificity

    The specificity of ab134152 was demonstrated in a Somatostatin Receptor 2-deficient mouse model. Refer to Fischer et al.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab171899)

  • Positive control

    • WB: Human and mouse brain tissue lysate. U937, INS-1 and Somatostatin Receptor 2 transfected HEK-293 cell lysates. IHC-P: Human pancreas and brain tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 75-80 kDa (predicted molecular weight: 41 kDa).Can be blocked with Somatostatin Receptor 2 peptide (ab171899).

This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 75-80 kDa (predicted molecular weight: 41 kDa).Can be blocked with Somatostatin Receptor 2 peptide (ab171899).

This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-Fr.

Target

  • Function

    Receptor for somatostatins-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. In RIN-5F cells, this receptor inhibits calcium entry by suppressing voltage dependent calcium-channels.

  • Tissue specificity

    Cerebrum and kidney. In lesser amounts in jejunum, colon and liver.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Somatostatin receptor type 2 antibody
    • SRIF-1 antibody
    • SRIF1 antibody
    • SS-2-R antibody
    • SS2-R antibody
    • SS2R antibody
    • SSR2_HUMAN antibody
    • SST2 antibody
    • SSTR2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)

    Immunohistochemical analysis of paraffin embedded human brain tissue labeled with ab134152 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    All lanes : Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution

    Lane 1 : U937 (Human histiocytic lymphoma monocyte) whole cell lysates prepared in RIPA lysis method
    Lane 2 : U937 (Human histiocytic lymphoma monocyte) whole cell lysates prepared in 1% SDS hot lysis method
    Lane 3 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
    Lane 4 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in 1% SDS hot lysis method
    Lane 5 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
    Lane 6 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in 1% SDS hot lysis method
    Lane 7 : A549 (Human lung carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 41 kDa
    Observed band size: 73 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST.

    This antibody doesn’t work in MCF-7 and MDA-MB-231 cells which should be positive (PMID: 16519802). A549 cell is Somatostatin Receptor 2 negative cell line (PMID: 19148511).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)Yuan et al Sci Rep. 2017; 7: 45617. Published online 2017 Apr 5. doi: 10.1038/srep45617

    Immunohistochemical analysis of paraffin-embedded human cervical cancer xenograft tissue (cells derived from liver metastases) using ab134152 at 1/100 dilution.

    Somatostatin Receptor 2 is detected using ab134152.

    From Figure 1 of Yuan et al.

    Yuan et al Sci Rep. 2017; 7: 45617. Published online 2017 Apr 5. doi: 10.1038/srep45617

    Reproduced under the Creative Commons Licence http://creativecommons.org/licenses/by/4.0/

  • Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    All lanes : Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution

    Lane 1 : Human brain lysates prepared in RIPA lysis method
    Lane 2 : Human brain lysates prepared in 1% SDS hot lysis method
    Lane 3 : Mouse brain lysates prepared in RIPA lysis method
    Lane 4 : Mouse brain lysates prepared in 1% SDS hot lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 41 kDa
    Observed band size: 73 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time:
    Lane 1 and 2: 1 second.
    Lane 3 and 4: 5 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)

    Immunohistochemical analysis of paraffin embedded human pancreas tissue labeled with ab134152 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    All lanes : Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution

    Lane 1 : U937 (Human histiocytic lymphoma cell line) cell lysates
    Lane 2 : INS-1 cell lysates
    Lane 3 : Somatostatin Receptor 2 transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 41 kDa

  • Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
    Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)

References (89)

Publishing research using ab134152? Please let us know so that we can cite the reference in this datasheet.

ab134152 has been referenced in 89 publications.

  • Csaba Z  et al. A simple novel approach for detecting blood-brain barrier permeability using GPCR internalization. Neuropathol Appl Neurobiol 47:297-315 (2021). PubMed: 32898926
  • Chen Y  et al. CDKN2A (p16INK4A) affects the anti-tumor effect of CDK inhibitor in somatotroph adenomas. Int J Mol Med 47:500-510 (2021). PubMed: 33416096
  • Bergmann R  et al. Development of a ghrelin receptor inverse agonist for positron emission tomography. Oncotarget 12:450-474 (2021). PubMed: 33747360
  • Meester EJ  et al. Autoradiographical assessment of inflammation-targeting radioligands for atherosclerosis imaging: potential for plaque phenotype identification. EJNMMI Res 11:27 (2021). PubMed: 33730311
  • Mayr CH  et al. Integrative analysis of cell state changes in lung fibrosis with peripheral protein biomarkers. EMBO Mol Med 13:e12871 (2021). PubMed: 33650774
View all Publications for this product

Customer reviews and Q&As

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Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Question

he data sheet indicates that the predicted band size for this antibody is 41 kDa, yet on the western-blot image for this antibody (on Abcam website) a band of 75 Kda is shown. Is it a mistake? Thank you for clarifying.

Read More
Answer

The unprocessed Somatostatin Receptor 2 is about 41kDa, and then its molecular weight increases to ˜80 kDa at maturity.

The specificity of the ab134152 was also examined in a sstr2-deficient mouse model. Please refer to the two publications attached (contact Scientific Support). They should help further clarify.

Read More

Question


Does this antibody detect a special variant of SSTR2 (SSTR2a or SSTR2b), or does it detect all of the variants.

Read More
Answer



This antibody, 3582-1/ab134152, reacts with an immunogen located at the C terminal residues of the canonical form of SSTR2. There is an alternate form of SSTR2 that expresses a different C-terminal sequence. Based on information in Uniprot, http://www.uniprot.org/uniprot/P30874#section_seq, it seems this antibody will not detect 'Isoform B' as defined on the webpage.

Read More

Question

Inquiry: My question concerns the Anti-Somatostatin Receptor 2 antibody UMB1. Does it bind to both sst2 splice variants sst2A and sst2B? Or just to sst2A? Looking forward to hearing from you.

Read More
Answer

I can confirm that there is no immunogenic homology with the SSTR2B protein so this antibody should bind to SSTR2A form only.

Read More

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