Recombinant Anti-Somatostatin Receptor 3/SSTR3 antibody [UMB5] (ab137026)
![Flow Cytometry (Intracellular) - Anti-Somatostatin Receptor 3/SSTR3 antibody [UMB5] (ab137026)](https://www.abcam.com/ps/products/137/ab137026/Images/ab137026-215885-anti-somatostatin-receptor-3-antibody-umb5-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Anti-Somatostatin Receptor 3/SSTR3 antibody [UMB5] (ab137026)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [UMB5] to Somatostatin Receptor 3/SSTR3
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Somatostatin Receptor 3/SSTR3 antibody [UMB5]
See all Somatostatin Receptor 3/SSTR3 primary antibodies -
Description
Rabbit monoclonal [UMB5] to Somatostatin Receptor 3/SSTR3 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Somatostatin Receptor 3/SSTR3 aa 350-450 (C terminal). The exact sequence is proprietary.
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Positive control
- Somatostatin Receptor 3/SSTR3 transfected 293T cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
UMB5 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 46 kDa.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Predicted molecular weight: 46 kDa. |
Target
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Function
Receptor for somatostatins-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. -
Tissue specificity
Brain, pituitary and pancreas. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsPhosphorylated. Phosphorylation increases upon somatostatin binding. -
Cellular localization
Cell membrane. Internalized into endoplasmic vesicles upon somatostatin-stimulation. - Information by UniProt
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Database links
- Entrez Gene: 6753 Human
- Omim: 182453 Human
- SwissProt: P32745 Human
- Unigene: 225995 Human
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Alternative names
- OTTHUMP00000028737 antibody
- Smstr 3 antibody
- Smstr3 antibody
see all
Images
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Flow Cytometry (Intracellular) - Anti-Somatostatin Receptor 3/SSTR3 antibody [UMB5] (ab137026)
Overlay histogram showing SHSY-5Y cells stained with ab137026 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab137026, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Western blot - Anti-Somatostatin Receptor 3/SSTR3 antibody [UMB5] (ab137026)](https://www.abcam.com/ps/products/137/ab137026/Images/ab137026-151493-anti-somatostatin-receptor-3-antibody-umb5-western-blot.jpg)
Western blot - Anti-Somatostatin Receptor 3/SSTR3 antibody [UMB5] (ab137026)All lanes : Anti-Somatostatin Receptor 3/SSTR3 antibody [UMB5] (ab137026) at 1/10000 dilution
Lane 1 : Non-transfected 293T cell lysate
Lane 2 : Somatostatin Receptor 3/SSTR3 transfected 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa -
![Anti-Somatostatin Receptor 3/SSTR3 antibody [UMB5] (ab137026)](https://www.abcam.com/ps/products/137/ab137026/Images/ab137026-3-benefits-of-recombinant-antibodies.png)
Anti-Somatostatin Receptor 3/SSTR3 antibody [UMB5] (ab137026)
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab137026 has been referenced in 18 publications.
- Wang S et al. Activation of Transcription Factor EB Alleviates Tubular Epithelial Cell Injury via Restoring Lysosomal Homeostasis in Diabetic Nephropathy. Oxid Med Cell Longev 2022:2812493 (2022). PubMed: 35082964
- Saishouji F et al. Ectopic ACTH-producing neuroendocrine tumor occurring with large recurrent metastatic pheochromocytoma: a case report. BMC Endocr Disord 22:184 (2022). PubMed: 35854271
- Majala S et al. Correlation of Somatostatin Receptor 1-5 Expression, [68Ga]Ga-DOTANOC, [18F]F-FDG PET/CT and Clinical Outcome in a Prospective Cohort of Pancreatic Neuroendocrine Neoplasms. Cancers (Basel) 14:N/A (2021). PubMed: 35008325
- Flores-Martinez Á et al. Quantitative Analysis of Somatostatin and Dopamine Receptors Gene Expression Levels in Non-functioning Pituitary Tumors and Association with Clinical and Molecular Aggressiveness Features. J Clin Med 9:N/A (2020). PubMed: 32971845
- Vázquez-Borrego MC et al. A Somatostatin Receptor Subtype-3 (SST3) Peptide Agonist Shows Antitumor Effects in Experimental Models of Nonfunctioning Pituitary Tumors. Clin Cancer Res 26:957-969 (2020). PubMed: 31624102