Recombinant Anti-Somatostatin Receptor 5 antibody [EPR7243] (ab156864)
![Western blot - Anti-Somatostatin Receptor 5 antibody [EPR7243] (ab156864)](https://www.abcam.com/ps/products/156/ab156864/Images/ab156864-164028-anti-somatostatin-receptor-5-antibody-epr7243-western-blot.jpg "Western blot - Anti-Somatostatin Receptor 5 antibody [EPR7243] (ab156864)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7243] to Somatostatin Receptor 5
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Somatostatin Receptor 5 antibody [EPR7243]
See all Somatostatin Receptor 5 primary antibodies -
Description
Rabbit monoclonal [EPR7243] to Somatostatin Receptor 5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa cells; Human fetal kidney, Human kidney, Human fetal liver, Human spleen, HepG2, HT29, 293T, HeLa, Human fetal heart and Human small intestine cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7243 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
|
| Flow Cyt |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa. |
|
Flow Cyt
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.
Target
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Function
Receptor for somatostatin 28 and to a lesser extent for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. -
Tissue specificity
Adult pituitary gland, heart, small intestine, adrenal gland, cerebellum and fetal hypothalamus. No expression in fetal or adult kidney, liver, pancreas, uterus, spleen, lung, thyroid or ovary. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 6755 Human
- Entrez Gene: 20609 Mouse
- Entrez Gene: 25354 Rat
- Omim: 182455 Human
- SwissProt: P35346 Human
- SwissProt: O08858 Mouse
- SwissProt: P30938 Rat
- Unigene: 449840 Human
see all -
Alternative names
- SSTR5 antibody
- Somatostatin receptor subtype 5 antibody
- Somatostatin receptor type 5 antibody
see all
Images
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Western blot - Anti-Somatostatin Receptor 5 antibody [EPR7243] (ab156864)All lanes : Anti-Somatostatin Receptor 5 antibody [EPR7243] (ab156864) at 1/1000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human kidney lysate
Lane 3 : Human fetal liver tissue lysate
Lane 4 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 39 kDa -
![Western blot - Anti-Somatostatin Receptor 5 antibody [EPR7243] (ab156864)](https://www.abcam.com/ps/products/156/ab156864/Images/ab156864-164027-anti-somatostatin-receptor-5-antibody-epr7243-western-blot.jpg)
Western blot - Anti-Somatostatin Receptor 5 antibody [EPR7243] (ab156864)All lanes : Anti-Somatostatin Receptor 5 antibody [EPR7243] (ab156864) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HT29 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Human fetal heart cell lysate
Lane 6 : Human small intestine lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 39 kDa -
![Flow Cytometry - Anti-Somatostatin Receptor 5 antibody [EPR7243] (ab156864)](https://www.abcam.com/ps/products/156/ab156864/Images/ab156864-164026-anti-somatostatin-receptor-5-antibody-epr7243-flow-cytometry.jpg)
Flow Cytometry - Anti-Somatostatin Receptor 5 antibody [EPR7243] (ab156864)Flow cytometric analysis of HeLa cells labeling Somatostatin receptor type 5 using ab156864 at 1/100 (red) or a rabbit IgG (negative) (green). -
![Anti-Somatostatin Receptor 5 antibody [EPR7243] (ab156864)](https://www.abcam.com/ps/products/156/ab156864/Images/ab156864-4-benefits-of-recombinant-antibodies.png)
Anti-Somatostatin Receptor 5 antibody [EPR7243] (ab156864)
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab156864 has been referenced in 2 publications.
- Ogawa S et al. Abundant expression of the membrane-anchored protease-regulator RECK in the anterior pituitary gland and its implication in the growth hormone/insulin-like growth factor 1 axis in mice. Mol Cell Endocrinol 508:110790 (2020). PubMed: 32165171
- Lee J et al. Effect of the Orally Active Growth Hormone Secretagogue MK-677 on Somatic Growth in Rats. Yonsei Med J 59:1174-1180 (2018). PubMed: 30450851