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    products/primary-antibodies/somatostatin-receptor-5-antibody-umb4-ab109495.pdf

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RecombinantRabMAb

Recombinant Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (34)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)
  • Western blot - Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)
  • Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [UMB4] to Somatostatin Receptor 5
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Anti-Somatostatin Receptor 3/SSTR3 antibody [UMB5] (ab137026)
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BSA Removal Kit - Nanoparticles (ab204912)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Somatostatin Receptor 5 antibody [UMB4]
    See all Somatostatin Receptor 5 primary antibodies
  • Description

    Rabbit monoclonal [UMB4] to Somatostatin Receptor 5
  • Host species

    Rabbit
  • Specificity

    Internal WB test in PANC-1, human pancreas and human brain lysates indicates this antibody might not detect endogenous Somatostatin Receptor 5.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab178801)

  • Positive control

    • WB: HT29 whole cell lysate (ab3952), 293T (Human embryonic kidney epithelial cell) transfected with human SSTR5 expression vector, IHC-P: Human pancreas
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    UMB4
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Neuropeptides
    • Hormones
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • More GPCR
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Neurology process
    • Neuroendocrinology
    • GH Regulation

Associated products

  • Alternative Versions

    • Anti-Somatostatin Receptor 5 antibody [UMB4] - BSA and Azide free (ab236074)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Immunizing Peptide (Blocking)

    • Somatostatin Receptor 5 peptide (ab178801)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HT-29 whole cell lysate (ab3952)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.Can be blocked with Somatostatin Receptor 5 peptide (ab178801).

Internal WB test in PANC-1, human pancreas and human brain lysates indicates this antibody might not detect endogenous Somatostatin Receptor 5.

IHC-P
1/50. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

For unpurified use at 1/100-1/250

See IHC antigen retrieval protocols.

Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.Can be blocked with Somatostatin Receptor 5 peptide (ab178801).

Internal WB test in PANC-1, human pancreas and human brain lysates indicates this antibody might not detect endogenous Somatostatin Receptor 5.

IHC-P
1/50. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

For unpurified use at 1/100-1/250

See IHC antigen retrieval protocols.

Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    Receptor for somatostatin 28 and to a lesser extent for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
  • Tissue specificity

    Adult pituitary gland, heart, small intestine, adrenal gland, cerebellum and fetal hypothalamus. No expression in fetal or adult kidney, liver, pancreas, uterus, spleen, lung, thyroid or ovary.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P35346 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6755 Human
    • Omim: 182455 Human
    • SwissProt: P35346 Human
    • Unigene: 449840 Human
    • Unigene: 728117 Human
    • Alternative names

      • SSTR5 antibody
      • Somatostatin receptor subtype 5 antibody
      • Somatostatin receptor type 5 antibody
      • SS 5R antibody
      • SS-5-R antibody
      • SS5 R antibody
      • SS5-R antibody
      • SS5R antibody
      • SSR5_HUMAN antibody
      • SST R5 antibody
      • SSTR 5 antibody
      • Sstr5 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas tissue sections labeling Somatostatin Receptor 5 with Purified ab109495 at 1:50 dilution (16.3 µg/ml). Heat mediated antigen retrieval was performed with Tris/EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins.. Performed on a Leica Biosystems BOND ® RX instrument.was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)Kaemmerer, D. et al BMC Cancer. 2017 Dec 28;17(1):896. doi: 10.1186/s12885-017-3911-3 Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

      Somatostatin receptor 2 (SSTR2), somatostatin receptor 5 (SSTR5) and CXCR4 expression in the hepatoblastoma cell line HepG2 and in the hepatoma cell lines Hep3B and HuH-7 in comparison to the small cell lung cancer cell line NCI-H69 and the neuroendocrine tumor cell line BON-1. Immunohistochemistry (red-brown color), counterstaining with hematoxylin; scale bar: 20 μm. Representative photomicrographs of three independent batches are shown.

      HepG2, Hep3B, HuH-7, NCI-H-69, and BON-1 cells (DSMZ, Braunschweig, Germany) were grown in 75 cm2 culture flasks to a confluency of 80%. Cells were washed once with phosphate-buffered saline and transferred into 10% buffered formalin (J.T.Baker, Deventer, The Netherlands) for 2 h. After centrifugation for 10 min at 3500 x g, the supernatant was removed, and 1 ml human pool plasma was added to the cell samples. After brief vortexing, 100 μl human fibrinogen (50–70% protein; ≥80% clottable) was added to each sample, and the samples were vortexed again. The resulting clots were placed for another 24 h in 10% buffered formalin and embedded in paraffin blocks.

      (After Kaemmerer, D. et al BMC Cancer. 2017 Dec 28;17(1):896. doi: 10.1186/s12885-017-3911-3).

      Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)

       

    • Western blot - Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)
      Western blot - Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)
      Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495) at 0.08 µg/ml (purified) + 293T (Human embryonic kidney epithelial cell) transfected with human SSTR5 expression vector, whole cell lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 39 kDa



      Blocking and diluting buffer: 5% NFDM/TBST
    • Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)
      Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (34)

    Publishing research using ab109495? Please let us know so that we can cite the reference in this datasheet.

    ab109495 has been referenced in 34 publications.

    • Ilie MD  et al. Gonadotroph Tumors Show Subtype Differences That Might Have Implications for Therapy. Cancers (Basel) 12:N/A (2020). PubMed: 32326042
    • Flores-Martinez Á  et al. Quantitative Analysis of Somatostatin and Dopamine Receptors Gene Expression Levels in Non-functioning Pituitary Tumors and Association with Clinical and Molecular Aggressiveness Features. J Clin Med 9:N/A (2020). PubMed: 32971845
    • Ehrstedt C  et al. Somatostatin receptor expression and mTOR pathway activation in glioneuronal tumours of childhood. Seizure 76:123-130 (2020). PubMed: 32062323
    • Lundsten S  et al. The HSP90 inhibitor onalespib potentiates 177Lu-DOTATATE therapy in neuroendocrine tumor cells. Int J Oncol 55:1287-1295 (2019). PubMed: 31638190
    • Muhammad A  et al. Pasireotide Responsiveness in Acromegaly Is Mainly Driven by Somatostatin Receptor Subtype 2 Expression. J Clin Endocrinol Metab 104:915-924 (2019). PubMed: 30346538
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    peptide pour ab109495?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 27 2013

    Answer


    J’ai contacté le laboratoire qui a accepté d’ajouter le peptide bloquant d’ab109495 au catalogue sous la référence ab178801.

    Read More

    Elisa Thomas

    Abcam Scientific Support

    Answered on Sep 27 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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