Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
![Immunocytochemistry/ Immunofluorescence - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)](https://www.abcam.com/ps/products/243/ab243043/Images/ab243043-331801-anti-sortilin-nt3-antibody-cl6526-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)")
Key features and details
- Mouse monoclonal [CL6526] to Sortilin/NT3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Sortilin/NT3 antibody [CL6526]
See all Sortilin/NT3 primary antibodies -
Description
Mouse monoclonal [CL6526] to Sortilin/NT3 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Sortilin/NT3 aa 600-750.
Database link: Q99523 -
Positive control
- IHC-P: Human cerebral cortex, testis, fallopian tube tissues; Mouse cerebral cortex and hippocampus tissue. WB: RH-30 and SK-MEL-30 cell lysate. ICC/IF: RH-30 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
CL6526 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/5000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 1 µg/ml.
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| ICC/IF |
Use a concentration of 2 - 10 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
| Notes |
|---|
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IHC-P
1/5000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 1 µg/ml. |
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ICC/IF
Use a concentration of 2 - 10 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Function
Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi. -
Tissue specificity
Expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis. Expressed at lower levels in lymphoid organs, kidney, colon and liver. -
Involvement in disease
Note=A common polymorphism located in a non-coding region between CELSR2 and PSRC1 alters a CEBP transcription factor binding site and is responsible for changes in hepatic expression of SORT1. Altered SORT1 expression in liver affects low density lipoprotein cholesterol levels in plasma and is associated with susceptibility to myocardial infarction. -
Sequence similarities
Belongs to the VPS10-related sortilin family. SORT1 subfamily.
Contains 9 BNR repeats. -
Domain
The N-terminal propeptide may facilitate precursor transport within the Golgi stack. Intrachain binding of the N-terminal propeptide and the extracellular domain may also inhibit premature ligand binding.
The extracellular domain may be shed following protease cleavage in some cell types. -
Post-translational
modificationsThe N-terminal propeptide is cleaved by furin and possibly other homologous proteases. -
Cellular localization
Membrane. Endoplasmic reticulum membrane. Endosome membrane. Golgi apparatus > Golgi stack membrane. Lysosome membrane. Nucleus membrane. Cell membrane. Lysosome membrane. Localized to membranes of the endoplasmic reticulum, endosomes, Golgi stack, lysosomes and nucleus. A small fraction of the protein is also localized to the plasma membrane. May also be found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. Localization to the plasma membrane in adipocytes may be enhanced by insulin. - Information by UniProt
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Database links
- Entrez Gene: 6272 Human
- Entrez Gene: 20661 Mouse
- Omim: 602458 Human
- SwissProt: Q99523 Human
- SwissProt: Q6PHU5 Mouse
- Unigene: 485195 Human
- Unigene: 703487 Human
- Unigene: 157119 Mouse
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Alternative names
- 100 kDa NT receptor antibody
- Glycoprotein 95 antibody
- Gp 95 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)
Paraformaldehyde-fixed, Triton X-100 permeabilized RH-30 cells stained for Sortilin/NT3 (Green) using ab243043 at 10 µg/ml in ICC/IF analysis. Microtubules are visulized in red, nucleus in blue.
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![Western blot - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)](https://www.abcam.com/ps/products/243/ab243043/Images/ab243043-451638-anti-sortilin-nt3-antibody-cl6526-western-blot-rh30-transfected-sirna-human.jpg)
Western blot - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)All lanes : Anti-Sortilin/NT3 antibody [CL6526] (ab243043) at 1 µg/ml
Lane 1 : RH-30 (Human bone marrow metastasis cell line) cells transfected with control siRNA
Lane 2 : RH-30 cells transfected with target specific siRNA probe #1
Lane 3 : RH-30 cells transfected with target specific siRNA probe #2Remaining relative intensity is presented.
Loading control: Anti-GAPDH
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)](https://www.abcam.com/ps/products/243/ab243043/Images/ab243043-331799-anti-sortilin-nt3-antibody-cl6526-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)Paraffin-embedded mouse hippocampus tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.
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![Western blot - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)](https://www.abcam.com/ps/products/243/ab243043/Images/ab243043-331800-anti-sortilin-nt3-antibody-cl6526-western-blot.jpg)
Western blot - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)Anti-Sortilin/NT3 antibody [CL6526] (ab243043) at 1 µg/ml + SK-MEL-30 cell lysate -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)](https://www.abcam.com/ps/products/243/ab243043/Images/ab243043-331798-anti-sortilin-nt3-antibody-cl6526-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)Paraffin-embedded mouse cerebral cortex tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)](https://www.abcam.com/ps/products/243/ab243043/Images/ab243043-331796-anti-sortilin-nt3-antibody-cl6526-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)Paraffin-embedded human fallopian tube tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)](https://www.abcam.com/ps/products/243/ab243043/Images/ab243043-331795-anti-sortilin-nt3-antibody-cl6526-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)Paraffin-embedded human testis tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)](https://www.abcam.com/ps/products/243/ab243043/Images/ab243043-331794-anti-sortilin-nt3-antibody-cl6526-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)Paraffin-embedded human cerebral cortex tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)](https://www.abcam.com/ps/products/243/ab243043/Images/ab243043-331797-anti-sortilin-nt3-antibody-cl6526-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [CL6526] (ab243043)Negative control:
Paraffin-embedded human lymph node tissue stained for Sortilin/NT3 using ab243043 at 1/5000 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab243043 has not yet been referenced specifically in any publications.