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    products/primary-antibodies/sos1-antibody-epr7480-ab140621.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Regulators
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-SOS1 antibody [EPR7480] (ab140621)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (4)

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Western blot - Anti-SOS1 antibody [EPR7480] (ab140621)
  • Western blot - Anti-SOS1 antibody [EPR7480] (ab140621)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOS1 antibody [EPR7480] (ab140621)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
  • Anti-SOS1 antibody [EPR7480] (ab140621)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7480] to SOS1
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

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Overview

  • Product name

    Anti-SOS1 antibody [EPR7480]
    See all SOS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7480] to SOS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human SOS1 aa 1050-1200.

  • Positive control

    • Raji, K562, HeLa and THP1 cell lysates; Human ovarian carcinoma tissue; Raji cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7480
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Regulators
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Ras family

Associated products

  • Alternative Versions

    • Anti-SOS1 antibody [EPR7480] - BSA and Azide free (ab248908)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • K-562 whole cell lysate (ab7911)
    • THP-1 whole cell lysate (ab7913)
  • Recombinant Protein

    • Recombinant Human SOS1 protein (His tag) (ab233594)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 152 kDa.
IHC-P (2)
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 152 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Promotes the exchange of Ras-bound GDP by GTP.
  • Tissue specificity

    Expressed in gingival tissues.
  • Involvement in disease

    Defects in SOS1 are the cause of gingival fibromatosis 1 (GGF1) [MIM:135300]; also known as GINGF1. Gingival fibromatosis is a rare overgrowth condition characterized by a benign, slowly progressive, nonhemorrhagic, fibrous enlargement of maxillary and mandibular keratinized gingiva. GGF1 is usually transmitted as an autosomal dominant trait, although sporadic cases are common.
    Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4) [MIM:610733]. NS4 is an autosomal dominant disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births. Rarely, NS4 is associated with juvenile myelomonocytic leukemia (JMML). SOS1 mutations engender a high prevalence of pulmonary valve disease; atrial septal defects are less common.
  • Sequence similarities

    Contains 1 DH (DBL-homology) domain.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 PH domain.
    Contains 1 Ras-GEF domain.
  • Target information above from: UniProt accession Q07889 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6654 Human
    • Entrez Gene: 20662 Mouse
    • Entrez Gene: 313845 Rat
    • Omim: 182530 Human
    • SwissProt: Q07889 Human
    • SwissProt: Q62245 Mouse
    • Unigene: 709893 Human
    • Unigene: 360004 Mouse
    • Unigene: 60975 Mouse
    see all
  • Alternative names

    • alternate SOS1 antibody
    • GF1 antibody
    • GGF1 antibody
    • GINGF antibody
    • gingival fibromatosis antibody
    • gingival fibromatosis hereditary 1 antibody
    • Guanine nucleotide exchange factor antibody
    • HGF antibody
    • NS4 antibody
    • Son of sevenless homolog 1 (Drosophila) antibody
    • Son of sevenless homolog 1 antibody
    • SOS Ras/Rac guanine nucleotide exchange factor 1 antibody
    • SOS-1 antibody
    • Sos1 antibody
    • SOS1_HUMAN antibody
    see all

Images

  • Western blot - Anti-SOS1 antibody [EPR7480] (ab140621)
    Western blot - Anti-SOS1 antibody [EPR7480] (ab140621)
    All lanes : Anti-SOS1 antibody [EPR7480] (ab140621) at 1/1000 dilution

    Lane 1 : Wild-type A431 cell lysate
    Lane 2 : SOS1 knockout A431 cell lysate
    Lane 3 : HEK-293 cell lysate
    Lane 4 : SK-OV-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 152 kDa
    Observed band size: 171 kDa why is the actual band size different from the predicted?



    False colour image of Western blot: Anti-SOS1 antibody [EPR7480] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab140621 was shown to bind specifically to SOS1. A band was observed at 171 kDa in wild-type A431 cell lysates with no signal observed at this size in SOS1 knockout cell line ab276087 (knockout cell lysate ab283833). To generate this image, wild-type and SOS1 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Twee® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

  • Western blot - Anti-SOS1 antibody [EPR7480] (ab140621)
    Western blot - Anti-SOS1 antibody [EPR7480] (ab140621)
    All lanes : Anti-SOS1 antibody [EPR7480] (ab140621) at 1/1000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : THP1 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 152 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labelling SOS1 with ab140621 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SOS1 antibody [EPR7480] (ab140621)
    Immunocytochemistry/ Immunofluorescence - Anti-SOS1 antibody [EPR7480] (ab140621)
    Immunofluorescent staining of Raji cells labelling SOS1 with ab140621 at 1/250 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

    Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab140621 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

    Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab140621 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

    Immunohistochemical analysis of paraffin embedded Human Lung adenocarcinoma tissue using ab140621 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

    Immunohistochemical analysis of paraffin embedded Human Glioma tissue using ab140621 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

    Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab140621 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-SOS1 antibody [EPR7480] (ab140621)
    Anti-SOS1 antibody [EPR7480] (ab140621)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (4)

Publishing research using ab140621? Please let us know so that we can cite the reference in this datasheet.

ab140621 has been referenced in 4 publications.

  • Cowan JR  et al. SOS1 Gain-of-Function Variants in Dilated Cardiomyopathy. Circ Genom Precis Med 13:e002892 (2020). PubMed: 32603605
  • Mishra M  et al. Adaptor Protein p66Shc: A Link Between Cytosolic and Mitochondrial Dysfunction in the Development of Diabetic Retinopathy. Antioxid Redox Signal 30:1621-1634 (2019). PubMed: 30105917
  • Mitsuda Y  et al. RUNX1 positively regulates the ErbB2/HER2 signaling pathway through modulating SOS1 expression in gastric cancer cells. Sci Rep 8:6423 (2018). PubMed: 29686309
  • Naidu S  et al. PDGFR-modulated miR-23b cluster and miR-125a-5p suppress lung tumorigenesis by targeting multiple components of KRAS and NF-kB pathways. Sci Rep 7:15441 (2017). PubMed: 29133857

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOS1 antibody [EPR7480]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (A549 tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
A549 tumor
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 03 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOS1 antibody [EPR7480]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9 target retrieval Dako
Permeabilization
No
Specification
Prostate cancer
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 01 2020

Western blot abreview for Anti-SOS1 antibody [EPR7480]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (HeLa and HEK293E)
Gel Running Conditions
Reduced Denaturing (3-8% Tris acetate)
Loading amount
3e+007 cells
Treatment
EGF and an inhibitor
Specification
HeLa and HEK293E
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Stacy Wilson

Verified customer

Submitted Dec 19 2019

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