Recombinant Anti-SOS1 antibody [EPR7480] (ab140621)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7480] to SOS1
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SOS1 antibody [EPR7480]
See all SOS1 primary antibodies -
Description
Rabbit monoclonal [EPR7480] to SOS1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human SOS1 aa 1050-1200.
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Positive control
- Raji, K562, HeLa and THP1 cell lysates; Human ovarian carcinoma tissue; Raji cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7480 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 152 kDa.
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IHC-P | (2) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 152 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
Target
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Function
Promotes the exchange of Ras-bound GDP by GTP. -
Tissue specificity
Expressed in gingival tissues. -
Involvement in disease
Defects in SOS1 are the cause of gingival fibromatosis 1 (GGF1) [MIM:135300]; also known as GINGF1. Gingival fibromatosis is a rare overgrowth condition characterized by a benign, slowly progressive, nonhemorrhagic, fibrous enlargement of maxillary and mandibular keratinized gingiva. GGF1 is usually transmitted as an autosomal dominant trait, although sporadic cases are common.
Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4) [MIM:610733]. NS4 is an autosomal dominant disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births. Rarely, NS4 is associated with juvenile myelomonocytic leukemia (JMML). SOS1 mutations engender a high prevalence of pulmonary valve disease; atrial septal defects are less common. -
Sequence similarities
Contains 1 DH (DBL-homology) domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 PH domain.
Contains 1 Ras-GEF domain. - Information by UniProt
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Database links
- Entrez Gene: 6654 Human
- Entrez Gene: 20662 Mouse
- Entrez Gene: 313845 Rat
- Omim: 182530 Human
- SwissProt: Q07889 Human
- SwissProt: Q62245 Mouse
- Unigene: 709893 Human
- Unigene: 360004 Mouse
see all -
Alternative names
- alternate SOS1 antibody
- GF1 antibody
- GGF1 antibody
see all
Images
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All lanes : Anti-SOS1 antibody [EPR7480] (ab140621) at 1/1000 dilution
Lane 1 : Wild-type A431 cell lysate
Lane 2 : SOS1 knockout A431 cell lysate
Lane 3 : HEK-293 cell lysate
Lane 4 : SK-OV-3 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 152 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-SOS1 antibody [EPR7480] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab140621 was shown to bind specifically to SOS1. A band was observed at 171 kDa in wild-type A431 cell lysates with no signal observed at this size in SOS1 knockout cell line ab276087 (knockout cell lysate ab283833). To generate this image, wild-type and SOS1 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Twee® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-SOS1 antibody [EPR7480] (ab140621) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 152 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labelling SOS1 with ab140621 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent staining of Raji cells labelling SOS1 with ab140621 at 1/250 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab140621 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab140621 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
Immunohistochemical analysis of paraffin embedded Human Lung adenocarcinoma tissue using ab140621 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
Immunohistochemical analysis of paraffin embedded Human Glioma tissue using ab140621 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab140621 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab140621 has been referenced in 6 publications.
- Waninger JJ et al. Biochemical characterization of the interaction between KRAS and Argonaute 2. Biochem Biophys Rep 29:101191 (2022). PubMed: 34988297
- Cowan JR et al. SOS1 Gain-of-Function Variants in Dilated Cardiomyopathy. Circ Genom Precis Med 13:e002892 (2020). PubMed: 32603605
- Mishra M et al. Adaptor Protein p66Shc: A Link Between Cytosolic and Mitochondrial Dysfunction in the Development of Diabetic Retinopathy. Antioxid Redox Signal 30:1621-1634 (2019). PubMed: 30105917
- Mitsuda Y et al. RUNX1 positively regulates the ErbB2/HER2 signaling pathway through modulating SOS1 expression in gastric cancer cells. Sci Rep 8:6423 (2018). PubMed: 29686309
- Naidu S et al. PDGFR-modulated miR-23b cluster and miR-125a-5p suppress lung tumorigenesis by targeting multiple components of KRAS and NF-kB pathways. Sci Rep 7:15441 (2017). PubMed: 29133857
- Baruzzi A et al. Sos1 Regulates Macrophage Podosome Assembly and Macrophage Invasive Capacity. J Immunol 195:4900-12 (2015). PubMed: 26447228