Recombinant Anti-SOX1 antibody [EPR4766] (ab109290)
![Western blot - Anti-SOX1 antibody [EPR4766] (ab109290)](https://www.abcam.com/ps/products/109/ab109290/Images/ab109290-478752-anti-sox1-antibody-epr4766-westernblot-human-mouse-rat.jpg "Western blot - Anti-SOX1 antibody [EPR4766] (ab109290)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4766] to SOX1
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-SOX1 antibody [EPR4766]
See all SOX1 primary antibodies -
Description
Rabbit monoclonal [EPR4766] to SOX1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4766 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
|
|
| ICC/IF | (1) |
1/100.
|
| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). |
|
ICC/IF
1/100. |
|
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Transcriptional activator. -
Tissue specificity
Mainly expressed in the developing central nervous system. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 6656 Human
- Entrez Gene: 20664 Mouse
- Omim: 602148 Human
- SwissProt: O00570 Human
- SwissProt: P53783 Mouse
- Unigene: 202526 Human
- Unigene: 39088 Mouse
-
Alternative names
- Sex determining region Y box 1 antibody
- sox1 antibody
- SOX1_HUMAN antibody
see all
Images
-
Western blot - Anti-SOX1 antibody [EPR4766] (ab109290)All lanes : Anti-SOX1 antibody [EPR4766] (ab109290) at 1/1000 dilution (Purified)
Lane 1 : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate
Lane 4 : Rat cerebral cortex lysate
Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 39 kDa -
![Flow Cytometry (Intracellular) - Anti-SOX1 antibody [EPR4766] (ab109290)](https://www.abcam.com/ps/products/109/ab109290/Images/ab109290-8-anti-sox1-antibody-epr4766-flowcytometry-nccit-human.jpg)
Flow Cytometry (Intracellular) - Anti-SOX1 antibody [EPR4766] (ab109290)Flow Cytometry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labelling SOX1 with Purified ab109290 at 1:100 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
-
![Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] (ab109290)](https://www.abcam.com/ps/products/109/ab109290/Images/ab109290-7-anti-sox1-antibody-epr4766-immunocytochemistry-nccit-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] (ab109290)Immunocytochemistry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling SOX1 with Purified ab109290 at 1:500 dilution (2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
-
![Anti-SOX1 antibody [EPR4766] (ab109290)](https://www.abcam.com/ps/products/109/ab109290/Images/ab109290-5-benefits-of-recombinant-antibodies.png)
Anti-SOX1 antibody [EPR4766] (ab109290)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (16)
ab109290 has been referenced in 16 publications.
- Luo C et al. CircFBXW7 inhibits the tumorigenesis of T-cell acute lymphoblastic leukemia through modulating miR-494-3p/SOX1 axis. Cell Death Discov 8:256 (2022). PubMed: 35538053
- Shen X et al. Hypoxia is fine-tuned by Hif-1α and regulates mesendoderm differentiation through the Wnt/β-Catenin pathway. BMC Biol 20:219 (2022). PubMed: 36199093
- Xu L et al. Neural stemness contributes to cell tumorigenicity. Cell Biosci 11:21 (2021). PubMed: 33468253
- Karahuseyinoglu S et al. Three-dimensional neuron-astrocyte construction on matrigel enhances establishment of functional voltage-gated sodium channels. J Neurochem 156:848-866 (2021). PubMed: 32939791
- Zhang J et al. Effect of NTN and Lmx1α on the Notch Signaling Pathway during the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Dopaminergic Neuron-Like Cells. Parkinsons Dis 2021:6676709 (2021). PubMed: 34373779