Name: Anti-SOX2 antibody [EPR3131]
Brand: RabMAb
SKU: ab92494
Rating: 5 (23 reviews)

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Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3131] to SOX2
Suitable for: WB, IHC - Wholemount, Sandwich ELISA, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human, Leucoraja erinacea

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

Product name

Anti-SOX2 antibody [EPR3131]
See all SOX2 primary antibodies
Description

Rabbit monoclonal [EPR3131] to SOX2
Host species

Rabbit
Specificity

The Rat recommendation is based on the ICC results. WB signal in rat samples are very weak. We do not guarantee WB for Rat.
Tested applications

Suitable for: WB, IHC - Wholemount, Sandwich ELISA, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivity

Reacts with: Mouse, Rat, Human, Leucoraja erinacea
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: NCCIT, F9, MCF-7 and C6 cell lysates; Human glioma lysate. IHC-P: Human gliocytoma, breast carcinoma, fetal stomach, fetal lung and embryonal carcinoma tissues; Sagittal maxillary incisor sections from E12, E13, E14, and E15 mouse embryos. ICC/IF: F9 and NCCIT cells; Mouse neuromesodermal progenitors. IHC-Wm: Leucoraja erinacea embryo; mouse blastocyst.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3131
Isotype

IgG
Research areas
- Neuroscience
- Development

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- MCF7 nuclear extract lysate (ab14860)
Related Products
- Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (ab116028)
- Recombinant Human SOX2 protein (ab79950)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(8)	1/1000 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
IHC - Wholemount	(3)	Use at an assay dependent concentration.
Sandwich ELISA		Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Rabbit monoclonal [EPR3131] to SOX2 (ab92494) as Capture.
IHC-P	(3)	1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/60.
ICC/IF	(3)	1/100.

Notes
WB 1/1000 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
IHC - Wholemount Use at an assay dependent concentration.
Sandwich ELISA Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Rabbit monoclonal [EPR3131] to SOX2 (ab92494) as Capture.
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/60.
ICC/IF 1/100.

Application notes

Is unsuitable for Flow Cyt or IP.

Function

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
Involvement in disease

Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
Sequence similarities

Contains 1 HMG box DNA-binding domain.
Post-translational
modifications

Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
Cellular localization

Nucleus.
Target information above from: UniProt accession P48431 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6657 Human
- Entrez Gene: 20674 Mouse
- Entrez Gene: 499593 Rat
- Omim: 184429 Human
- SwissProt: P48431 Human
- SwissProt: P48432 Mouse
- Unigene: 518438 Human
- Unigene: 65396 Mouse
Alternative names
- ANOP3 antibody
- cb236 antibody
- Delta EF2a antibody
- lcc antibody
- MCOPS3 antibody
- MGC148683 antibody
- MGC2413 antibody
- RGD1565646 antibody
- Sex determining region Y box 2 antibody
- SOX 2 antibody
- Sox2 antibody
- SOX2_HUMAN antibody
- SRY (sex determining region Y) box 2 antibody
- SRY box containing gene 2 antibody
- SRY related HMG box 2 antibody
- SRY related HMG box gene 2 antibody
- SRY-box 2 antibody
- Transcription factor SOX 2 antibody
- Transcription factor SOX-2 antibody
- ysb antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)

Confocal image showing nuclear staining on F9 cells

Ab92494 staining SOX2 in the F9 (mouse embryonal carcinoma) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor^® 488-conjugated Goat anti-Rabbit IgG, Ab150077 (1/1000) was used as the secondary antibody. Counterstained with Ab7291 anti-Tubulin (1/1000), Ab150120 AlexaFluor^®594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

Negative control 1 Ab92494 was used as the primary antibody at 1/200 and Ab150120 was used as the secondary at 1/1000.

Negative control 2 Ab7291was used as the primary antibody at 1/1000 and Ab150077 was used as the secondary at 1/1000.
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)

ab92494 staining SOX2 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab92494 at 1/100 dilution and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)Image from Gouti M et al., PLoS Biol. 2014;12(8):e1001937. Fig 2.; doi: 10.1371/journal.pbio.1001937. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Transient Wnt and FGF signalling induce dual fated mouse neuromesodermal progenitors.

Immunostaining of cells treated with FGF/Wnt revealed the coexpression of Brachyury with Sox2 (NMPs). In the absence of Wnt, NPCs express Sox2 but the expression of Brachyury is only evident in a very small proportion of cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)Image from Togo Y et al., PLoS One. 2016;11(8):e0161067. Fig 6.; doi: 10.1371/journal.pone.0161067. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

SOX2 immunostaining in sagittal maxillary incisor sections from E12 (A-D), E13 (E-H), E14 (I-L), and E15 (M-P) embryos.

At E13, strong SOX2 staining was seen in the lingual region of the epithelial dental lamina in all mice (E, G & H) except for the Usag-1^+/+/Runx2^-/- mice, in which SOX2 was found throughout the dental lamina (F). At E15, strong SOX2 staining was seen in the additional lingual bud in the Usag-1^+/+/Runx2^-/- mice (N).
Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)

All lanes : Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution

Lane 1 : NCCIT (human pluripotent embryonic carcinoma cell line) whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lane 3 : SK-OV-3 (human ovarian cancer cell line) whole cell lysate
Lane 4 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 5 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 7 : Human breast cancer tissue lysate
Lane 8 : Human glioma lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 34 kDa
Observed band size: 34 kDa

Exposure time: 3 minutes

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)

Confocal image showing nuclear staining on NCCIT cells

Ab92494 staining SOX2 in NCCIT cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor^® 488-conjugated Goat anti-Rabbit IgG, Ab150077 (1/1000) was used as the secondary antibody. Counterstained with Ab7291 anti-Tubulin (1/1000), Ab150120 AlexaFluor^®594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

Negative control 1 Ab92494 was used as the primary antibody at 1/200 and Ab150120 was used as the secondary at 1/1000.

Negative control 2 Ab7291was used as the primary antibody at 1/1000 and Ab150077 was used as the secondary at 1/1000.
Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)

All lanes : Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution

Lane 1 : F9 (mouse embryonic testicular cancer cell line) whole cell lysate
Lane 2 : 4T1 (mouse mammary gland carcinoma cell line) whole cell lysate
Lane 3 : Mouse hippocampus lysate
Lane 4 : C6 (rat glial tumor cell line) whole cell lysate
Lane 5 : Rat hippocampus lysate
Lane 6 : Rat spinal cord lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 34 kDa
Observed band size: 34 kDa

Exposure time: 3 minutes

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [EPR3131] (ab92494)

Confocal image showing negative staining on NIH/3T3 cells.

Ab92494 staining SOX2 in the NIH/3T3 (mouse embryonic fibroblast cell line) (negative control) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton-X. Samples were incubated with primary antibody (1/200). An Alexa Fluor^® 488-conjugated Goat anti-Rabbit IgG, ab150077 (1/1000) was used as the secondary antibody. Counterstained with ab7291 anti-Tubulin (1/1000), Ab150120 Alexa Fluor^® 594 Goat anti-Mouse secondary (1/1000). DAPI was used as a nuclear counter stain.

Negative control 1: ab92494 was used as the primary antibody at 1/200 and ab150120 was used as the secondary at 1/1000.

Negative control 2: ab7291was used as the primary antibody at 1/1000 and ab150077 was used as the secondary at 1/1000.
Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)

Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1000 dilution (unpurified) + NCCIT (human pluripotent embryonic carcinoma cell line) cell lysate at 10 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 34 kDa
Observed band size: 34 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)

Anti-SOX2 antibody [EPR3131] (ab92494) at 1/1500 dilution (purified) + F9 (mouse embryonic testicular cancer cell line) cell lysate at 10 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 34 kDa
Observed band size: 34 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-SOX2 antibody [EPR3131] (ab92494)

All lanes : Anti-SOX2 antibody [EPR3131] (ab92494) at 1/5000 dilution (unpurified)

Lane 1 : NCCIT (human pluripotent embryonic carcinoma cell line) cell lysate
Lane 2 : MCF-7 (human breast adenocarcinoma cell line) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling SOX2 with unpurified ab92494 at 1/60. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling SOX2 with purified ab92494 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SOX2 with unpurified ab92494.

Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal stomach tissue labelling SOX2 with unpurified ab92494.

Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal lung tissue labelling SOX2 with unpurified ab92494.

Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human embryonal carcinoma tissue labelling SOX2 with unpurified ab92494.

Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of normal human lung tissue. Unpurified ab92494 shows negative staining.

Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131] (ab92494)

Negative control: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of negative human seminoma tissue using unpurified ab92494.

Heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 was performed before commencing with IHC staining protocol.
IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (ab92494)Image courtesy of Dr. Gillis, Dalhousie University, Canada.

IHC - Wholemount analysis of Leucoraja erinacea embryo labelling SOX2 with unpurified ab92494 at 1/200. The sample was incubated with the primary antibody for 48 hours at 4°C in 10% fetal calf serum in PBT. Detection: DAB.
See Abreview
IHC - Wholemount - Anti-SOX2 antibody [EPR3131] (ab92494)This image is courtesy of an anonymous Abreview.

IHC - Wholemount analysis of mouse blastocyst labelling SOX2 (pink) with unpurified ab92494 at 1/200. The sample was incubated with the primary antibody for 48 hours at 4°C. Nuclei stained with DAPI (grey).
See Abreview
Sandwich ELISA - Anti-SOX2 antibody [EPR3131] (ab92494)

Standard Curve for SOX2 (Analyte: SOX2 protein (Human) (ab79950)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [57CT23.3.4] to SOX2 (ab75485) at 0.2µg/ml and Detector Antibody Rabbit monoclonal [EPR3131] to SOX2 (ab92494) at 0.5µg/ml.
Anti-SOX2 antibody [EPR3131] (ab92494)

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols
Protocol Booklet

Click here to view the general protocols

SDS download

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Language
Datasheet download

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Publishing research using ab92494? Please let us know so that we can cite the reference in this datasheet.

ab92494 has been referenced in 196 publications.

Yan S et al. Extracellular magnetic labeling of biomimetic hydrogel-induced human mesenchymal stem cell spheroids with ferumoxytol for MRI tracking. Bioact Mater 19:418-428 (2023). PubMed: 35574059
Schembs L et al. The ciliary gene INPP5E confers dorsal telencephalic identity to human cortical organoids by negatively regulating Sonic hedgehog signaling. Cell Rep 39:110811 (2022). PubMed: 35584663
Fei Z et al. Krüppel-like factor 4 promotes the proliferation and osteogenic differentiation of BMSCs through SOX2/IGF2 pathway. Acta Biochim Pol 69:349-355 (2022). PubMed: 35617351
Chen X et al. VEGF-Loaded Heparinised Gelatine-Hydroxyapatite-Tricalcium Phosphate Scaffold Accelerates Bone Regeneration via Enhancing Osteogenesis-Angiogenesis Coupling. Front Bioeng Biotechnol 10:915181 (2022). PubMed: 35757798
Li H et al. Chelerythrine Chloride Inhibits Stemness of Melanoma Cancer Stem-Like Cells (CSCs) Potentially via Inducing Reactive Oxygen Species and Causing Mitochondria Dysfunction. Comput Math Methods Med 2022:4000733 (2022). PubMed: 35761835

View all Publications for this product

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1-10 of 28 Abreviews or Q&A

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Brain)

Permeabilization

Yes - Triton 0.1%

Specification

Brain

Blocking step

Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 20°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 20 2023

Application

Western blot

Sample

Human Cell lysate - whole cell (Oesophagus)

Gel Running Conditions

Reduced Denaturing (4-12%)

Loading amount

20 µg

Specification

Oesophagus

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Helena Santos-Rosa

Verified customer

Submitted Oct 11 2022

Application

Immunocytochemistry

Sample

Human (Embryonic stem cells)

Permeabilization

Yes - methanol

Specification

Embryonic stem cells

Blocking step

Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 21 2021

Application

Immunocytochemistry/ Immunofluorescence

Sample

Pig Cell (embryo)

Permeabilization

Yes - tritonx-100

Specification

embryo

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 23 2021

Application

Immunohistochemistry free floating

Sample

Mouse Tissue sections (Brain)

Specification

Brain

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 26 2021

Application

Immunohistochemistry free floating

Sample

Rat Tissue sections (Cerebellum)

Specification

Cerebellum

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 26 2021

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Rat Tissue sections (Cerebellum)

Antigen retrieval step

None

Permeabilization

Yes - PBS 1x, Triton x-100 1%

Specification

Cerebellum

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 20 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK293)

Gel Running Conditions

Reduced Denaturing (4-15% gradient)

Loading amount

20 µg

Specification

HEK293

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 27 2020

Application

Western blot

Sample

Mouse Tissue lysate - whole (Brain)

Gel Running Conditions

Reduced Denaturing (4-15% gradient)

Loading amount

40 µg

Specification

Brain

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 26 2020

Application

Western blot

Sample

Rat Cell lysate - whole cell (Hippocampal)

Gel Running Conditions

Reduced Denaturing (4-15% gradient)

Loading amount

40 µg

Specification

Hippocampal

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

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Submitted Feb 26 2020

1-10 of 28 Abreviews or Q&A

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Related Products

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (196)

Customer reviews and Q&As

Immunohistochemistry (Frozen sections) abreview for Anti-SOX2 antibody [EPR3131]

Western blot abreview for Anti-SOX2 antibody [EPR3131]

Immunocytochemistry abreview for Anti-SOX2 antibody [EPR3131]

Immunocytochemistry/ Immunofluorescence abreview for Anti-SOX2 antibody [EPR3131]

Immunohistochemistry free floating abreview for Anti-SOX2 antibody [EPR3131]

Immunohistochemistry free floating abreview for Anti-SOX2 antibody [EPR3131]

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX2 antibody [EPR3131]

Western blot abreview for Anti-SOX2 antibody [EPR3131]

Western blot abreview for Anti-SOX2 antibody [EPR3131]

Western blot abreview for Anti-SOX2 antibody [EPR3131]

Post-translational
modifications