Name: Anti-SOX2 antibody [SP76]
Brand: RabMAb
SKU: ab93689
Rating: 4 (1 reviews)

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Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [SP76] (ab93689)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP76] to SOX2
Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

Product name

Anti-SOX2 antibody [SP76]
See all SOX2 primary antibodies
Description

Rabbit monoclonal [SP76] to SOX2
Host species

Rabbit
Tested applications

Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human SOX2 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P48431
Positive control
- IHC-P: Human prostate, newborn brain, Human lung carcinoma, Mouse stomach, and Rat stomach tissue; WB: MCF7 cell lysate; Flow Cyt: F9, and NCCIT cells; ICC: F9, and NCCIT cells.
General notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP76
Isotype

IgG
Research areas
- Neuroscience
- Development

Alternative Versions
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab93689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/100. Antigen Retrieval is recommended, boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes.
Flow Cyt		1/200.
ICC/IF		1/50.
WB		1/100. Predicted molecular weight: 34 kDa.

Notes
IHC-P 1/100. Antigen Retrieval is recommended, boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes.
Flow Cyt 1/200.
ICC/IF 1/50.
WB 1/100. Predicted molecular weight: 34 kDa.

Function

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
Involvement in disease

Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
Sequence similarities

Contains 1 HMG box DNA-binding domain.
Post-translational
modifications

Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
Cellular localization

Nucleus.
Target information above from: UniProt accession P48431 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6657 Human
- Entrez Gene: 20674 Mouse
- Entrez Gene: 499593 Rat
- Omim: 184429 Human
- SwissProt: P48431 Human
- SwissProt: P48432 Mouse
- Unigene: 518438 Human
- Unigene: 65396 Mouse
Alternative names
- ANOP3 antibody
- cb236 antibody
- Delta EF2a antibody
- lcc antibody
- MCOPS3 antibody
- MGC148683 antibody
- MGC2413 antibody
- RGD1565646 antibody
- Sex determining region Y box 2 antibody
- SOX 2 antibody
- Sox2 antibody
- SOX2_HUMAN antibody
- SRY (sex determining region Y) box 2 antibody
- SRY box containing gene 2 antibody
- SRY related HMG box 2 antibody
- SRY related HMG box gene 2 antibody
- SRY-box 2 antibody
- Transcription factor SOX 2 antibody
- Transcription factor SOX-2 antibody
- ysb antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [SP76] (ab93689)

Immunocytochemistry/ Immunofluorescence analysis of NCCIT( human pluripotent embryonic carcinoma epithelial cell) cells labeling SOX2 with purified ab93689 at 1:50 (2.8 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [SP76] (ab93689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling SOX2 with ab93689 at 1/100 dilution (1.37 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow Cytometry - Anti-SOX2 antibody [SP76] (ab93689)

Flow Cytometry analysis of F9(Mouse embryonal carcinoma epithelial cell) cells labeling SOX2 with purified ab93689 at 1:200 dilution (0.685 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [SP76] (ab93689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse stomach tissue sections labeling SOX2 with ab93689 at 1/100 dilution (1.37 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [SP76] (ab93689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling SOX2 with ab93689 at 1/100 dilution (1.37 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [SP76] (ab93689)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate tissue sections labeling SOX2 with ab93689 at 1/100 dilution (1.37 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody [SP76] (ab93689)

Immunocytochemistry/ Immunofluorescence analysis of F9 (mouse embryonal carcinoma epithelial cell) cells labeling SOX2 with purified ab93689 at 1/50 (2.8 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-SOX2 antibody [SP76] (ab93689)

Flow Cytometry analysis of NCCIT(Human pluripotent embryonic carcinoma epithelial cell) cells labeling SOX2 with purified ab93689 at 1/200 dilution (0.685 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabeled control - Unlabelled cells (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [SP76] (ab93689)

Immunohistochemical analysis of human prostate tissue labeling SOX2 with ab93689.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [SP76] (ab93689)

Formalin-fixed paraffin-embedded human newborn brain tissue stained for SOX2 using ab93689 at 1/100 dilution in immunohistochemical analysis.
Western blot - Anti-SOX2 antibody [SP76] (ab93689)

Anti-SOX2 antibody [SP76] (ab93689) at 1/100 dilution + MCF7 (Human breast adenocarcinoma cell line) cell lysate

Predicted band size: 34 kDa
Observed band size: 34 kDa
Anti-SOX2 antibody [SP76] (ab93689)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab93689? Please let us know so that we can cite the reference in this datasheet.

ab93689 has been referenced in 28 publications.

Gu J et al. Plasmalogens Eliminate Aging-Associated Synaptic Defects and Microglia-Mediated Neuroinflammation in Mice. Front Mol Biosci 9:815320 (2022). PubMed: 35281262
Sun J & Zhang W Supplementation with dietary omega-3 PUFA mitigates fetal brain inflammation and mitochondrial damage caused by high doses of sodium nitrite in maternal rats. PLoS One 17:e0266084 (2022). PubMed: 35324981
Koide T et al. CDX2-induced intestinal metaplasia in human gastric organoids derived from induced pluripotent stem cells. iScience 25:104314 (2022). PubMed: 35602937
Kawasaki Y et al. GPNMB-Positive Cells in Head and Neck Squamous Cell Carcinoma-Their Roles in Cancer Stemness, Therapy Resistance, and Metastasis. Pathol Oncol Res 28:1610450 (2022). PubMed: 36061142
Mauro-Lizcano M et al. SOX2-high cancer cells exhibit an aggressive phenotype, with increases in stemness, proliferation and invasion, as well as higher metabolic activity and ATP production. Aging (Albany NY) 14:9877-9889 (2022). PubMed: 36566021

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Customer reviews and Q&As

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Application

IHC - Wholemount

Sample

Human Tissue (Breast cancer tissue)

Specification

Breast cancer tissue

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 28 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Purification notes

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (28)

Customer reviews and Q&As

IHC - Wholemount abreview for Anti-SOX2 antibody [SP76]

Post-translational
modifications