Recombinant Anti-SPARC antibody [SP205] (ab225716)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP205] to SPARC
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human

Carrier Free

Product name

Anti-SPARC antibody [SP205]
See all SPARC primary antibodies
Description

Rabbit monoclonal [SP205] to SPARC
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivity

Reacts with: Human
Predicted to work with: Rabbit, Cow, Pig
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human kidney, stomach adenocarcinoma, colon adenocarcinoma, renal cell carcinoma, breast ductal carcinoma, lung squamos cell carcinoma, pancreatic adenocarcinoma, tonsil, placenta, testis and cerebellum tissues. WB: HUVEC and Jurkat whole cell lysate (ab7899). Flow Cyt: A375 cells. ICC/IF: A375 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP205
Isotype

IgG
Research areas

Alternative Versions
- Anti-SPARC antibody [SP205] - BSA and Azide free (ab245733)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Jurkat whole cell lysate (ab30128)
- Jurkat whole cell lysate (ab7899)
Recombinant Protein
- Recombinant human SPARC protein (ab107952)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab225716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/400. Predicted molecular weight: 35 kDa. Primary incubation for 1 hour at room temperature.
IHC-P		1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary incubation for 10 minutes at room temperature.
Flow Cyt		1/200.
ICC/IF		1/15.

Notes
WB 1/400. Predicted molecular weight: 35 kDa. Primary incubation for 1 hour at room temperature.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary incubation for 10 minutes at room temperature.
Flow Cyt 1/200.
ICC/IF 1/15.

Function

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Sequence similarities

Belongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
Developmental stage

Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
Cellular localization

Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
Target information above from: UniProt accession P09486 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 282077 Cow
- Entrez Gene: 6678 Human
- Entrez Gene: 595124 Pig
- Entrez Gene: 100009355 Rabbit
- Omim: 182120 Human
- SwissProt: P13213 Cow
- SwissProt: P09486 Human
- SwissProt: P20112 Pig
- SwissProt: P36233 Rabbit
- Unigene: 111779 Human
- Unigene: 708558 Human
see all
Alternative names
- AA517111 antibody
- Basement membrane protein 40 antibody
- Basement-membrane protein 40 antibody
- BM 40 antibody
- BM-40 antibody
- BM40 antibody
- Cysteine rich protein antibody
- hm:zeh0062 antibody
- MGC128090 antibody
- OI17 antibody
- ON antibody
- Osteonectin antibody
- Secreted acidic cystein rich glycoprotein antibody
- Secreted protein acidic and cysteine rich antibody
- Secreted protein acidic and rich in cysteine antibody
- Secreted protein acidic cysteine rich (osteonectin) antibody
- Secreted protein acidic cysteine rich antibody
- SPARC antibody
- SPRC antibody
- SPRC_HUMAN antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

Formalin-fixed, paraffin-embedded human kidney tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Flow Cytometry - Anti-SPARC antibody [SP205] (ab225716)

Flow Cytometry analysis of A375(Human malignant melanoma epithelial cell) cells labeling SPARC with purified ab225716 at 1:200 dilution (0.595 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
Immunocytochemistry/ Immunofluorescence - Anti-SPARC antibody [SP205] (ab225716)

Immunocytochemistry/ Immunofluorescence analysis of A375 (human malignant melanoma epithelial cell) cells labeling SPARC with purified ab225716 at 1:15 (7.9 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-SPARC antibody [SP205] (ab225716)

All lanes : Anti-SPARC antibody [SP205] (ab225716) at 1/400 dilution

Lanes 1 & 3 : HUVEC (human umbilical vein endothelial cell line) cell lysate
Lanes 2 & 4 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

Predicted band size: 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

Formalin-fixed, paraffin-embedded human lung squamos cell carcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

Formalin-fixed, paraffin-embedded human placenta tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

Formalin-fixed, paraffin-embedded human testis tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody [SP205] (ab225716)

Formalin-fixed, paraffin-embedded human cerebellum tissue stained for SPARC using ab225716 at 1/100 dilution in immunohistochemical analysis.
Anti-SPARC antibody [SP205] (ab225716)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab225716? Please let us know so that we can cite the reference in this datasheet.

ab225716 has been referenced in 3 publications.

Wang L et al. Low Levels of SPARC are Associated with Tumor Progression and Poor Prognosis in Human Endometrial Carcinoma. Onco Targets Ther 13:11549-11569 (2020). PubMed: 33204109
Fu Y et al. Glucose affects cell viability, migration, angiogenesis and cellular adhesion of human retinal capillary endothelial cells via SPARC. Exp Ther Med 17:273-283 (2019). PubMed: 30651792
Xu J et al. SPARC correlates with unfavorable outcome and promotes tumor growth in lung squamous cell carcinoma. Exp Mol Pathol 110:104276 (2019). PubMed: 31233732

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Purification notes

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Developmental stage

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (3)

Customer reviews and Q&As